Screening of potential uranium protein targets in fish ovaries after chronic waterborne exposure: Differences and similarities between roach and zebrafish

Abstract

Screening of potential uranium protein targets in fish ovaries after chronic waterborne exposure: differences and similarities between roach and zebrafish.

Atomic Spectrometry Update: review of advances in elemental speciation

“…Sample preparation and analysis procedures involving SEC- ICP-SFMS , already developed and reported by Eb-Levadoux and co-workers, were applied to the screening of potential U protein targets in fish ovaries after chronic waterborne exposure. 195 Metal–protein complexes were extracted from freshly frozen gonad samples (approx. 65 mg) with 1800 μL of 25 mmol L −1 HEPES + 250 mmol L −1 sucrose at pH 7.4 with a Potter–Elvehjem homogeniser for 3 min at 0 °C.…”

“…Sample preparation and analysis procedures involving SEC-ICP-SFMS, already developed and reported by Eb-Levadoux and co-workers, were applied to the screening of potential U protein targets in sh ovaries aer chronic waterborne exposure. 195 Metal-protein complexes were extracted from freshly frozen gonad samples (approx. 65 mg) with 1800 mL of 25 mmol L À1 HEPES + 250 mmol L À1 sucrose at pH 7.4 with a Potter-Elvehjem homogeniser for 3 min at 0 C. The cytosolic metal-protein complexes, contained in the supernatant, were separated by centrifugation, and injected into the chromatographic system consisting of a Superdex 200 10/300 GL column with successive detection by UV spectrophotometry (280 nm) and ICP-MS.…”

Metal-binding biomolecules in the liver of northern pike (Esox lucius Linnaeus, 1758): The first data for the family Esocidae

“…Usually the studies are aimed at analysis of one specific biomolecule, and very often that is MT and its association with various metals within the cell (Goenaga Infante et al, 2003, 2006Van Campenhout et al, 2004, 2008Huang et al, 2007;Hauser-Davis et al, 2014), or at analysis of one metal and all the biomolecules that bind it (e.g. uranium; Bucher et al, 2014;Frelon et al, 2020). More systematic approaches, which would provide deeper insight in behaviour of higher number of metals within the organs and cells of various fish species, are not often encountered.…”