Screening of Potential Xanthine Oxidase Inhibitors in Gnaphalium hypoleucum DC. by Immobilized Metal Affinity Chromatography and Ultrafiltration-Ultra Performance Liquid Chromatography-Mass Spectrometry

Zhang, Hongjian; Hu, Yi‐Juan; Xu, Ping; Liang, Wei-qing; Zhou, Jie; Liu, Pei-Gang; Lin, Cheng Huang; Pu, Jinbao

doi:10.3390/molecules21091242

Cited by 20 publications

(15 citation statements)

References 14 publications

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“…Interestingly, the complex had a more remarkable hypouricemic effect than luteolin and both compounds proved to be noncytotoxic to a human liver cancer cell line [70]. A glycosylated derivative, luteolin-4′-glucoside (Figure 18), also inhibited XO, with an in vitro IC 50 value of 0.26 μg/mL [71]. In addition, this molecule had potent uricosuric effects in hyperuricemic mice with renal mURAT1 and decreasing XO activity, as well as anti-inflammatory effects.…”

Section: Phenolic Compounds and Analoguesmentioning

confidence: 99%

From Xanthine Oxidase Inhibition to In Vivo Hypouricemic Effect: An Integrated Overview of In Vitro and In Vivo Studies with Focus on Natural Molecules and Analogues

Serrano

Figueiredo

Almeida

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Hyperuricemia is characterized by elevated uric acid (UA) levels on blood, which can lead to gout, a common pathology. These high UA levels are associated with increased purine ingestion and metabolization and/or its decreased excretion. In this field, xanthine oxidase (XO), by converting hypoxanthine and xanthine to UA, plays an important role in hyperuricemia control. Based on limitations and adverse effects associated with the use of allopurinol and febuxostat, the most known approved drugs with XO inhibitory effect, the search for new molecules with XO activity is growing. However, despite the high number of studies, it was found that the majority of tested products with relevant XO inhibition were left out, and no further pharmacological evaluation was performed. Thus, in the present review, available information published in the past six years concerning isolated molecules with in vitro XO inhibition complemented with cytotoxicity evaluation as well as other relevant studies, including in vivo hypouricemic effect, and pharmacokinetic/pharmacodynamic profile was compiled. Interestingly, the analysis of data collected demonstrated that molecules from natural sources or their mimetics and semisynthetic derivatives constitute the majority of compounds being explored at the moment by means of in vitro and in vivo animal studies. Therefore, several of these molecules can be useful as lead compounds and some of them can even have the potential to be considered in the future clinical candidates for the treatment of hyperuricemia.

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Section: Phenolic Compounds and Analoguesmentioning

confidence: 99%

From Xanthine Oxidase Inhibition to In Vivo Hypouricemic Effect: An Integrated Overview of In Vitro and In Vivo Studies with Focus on Natural Molecules and Analogues

Serrano

Figueiredo

Almeida

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…The ligands were then prepared for docking by computing the charges, and the structures were saved in PDBQT file format via AutoDock 4.2. Molecular docking experiment of ligands was performed following the protocol described by Zhang et al [ 25 ]. The size and center of the coordinates of the grid box was first validated by re-docking the co-crystallized ligand into the active sites of the receptor.…”

Section: Methodsmentioning

confidence: 99%

Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach

et al. 2018

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The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p < 0.05), as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p < 0.05). Afzelechin 3-O-alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.

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“…hypoleucum DC. is a folk medicine used for the treatment of gout in china and also used as anti-inflammatory, antitussive, gout and expectorant activities 12,18 . No comprehensive scientific study has been carried out on G. hypoleucum for their wound healing activity so far, hence the present study was undertaken to estimate the wound healing potential and compare their efficacy.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Wound Healing Potential of Gnaphalium Hypoleucum Dc.

Kumar¹,

Singh²,

Sharma³

et al. 2018

Int. Res. J. Pharm.

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The wound healing potential of Gnaphalium hypoleucum was estimated by excision and incision wound models. This study was designed to prove the traditional believe of this plant as the wound healer. This screening showed that Gnaphalium hypoleucum possesses highly significant wound healing potential, almost 98 % in 13 days study. Not only the faster healing was reported but also the quality of healing was improved when compared with standard as well as control group. On 13th day, excision wound was completely healed (99.33%) while incision wound showed significant tensile strength (588.33 gm) as control group (383.33gm). The epithelization time was significantly reduced to 7.33 days as in control (11.33 days) while wound index study showed that quality of healing of wound was highly improved (0.33) as compared to control (3.33) group.

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Screening of Potential Xanthine Oxidase Inhibitors in Gnaphalium hypoleucum DC. by Immobilized Metal Affinity Chromatography and Ultrafiltration-Ultra Performance Liquid Chromatography-Mass Spectrometry

Cited by 20 publications

References 14 publications

From Xanthine Oxidase Inhibition to In Vivo Hypouricemic Effect: An Integrated Overview of In Vitro and In Vivo Studies with Focus on Natural Molecules and Analogues

From Xanthine Oxidase Inhibition to In Vivo Hypouricemic Effect: An Integrated Overview of In Vitro and In Vivo Studies with Focus on Natural Molecules and Analogues

Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach

Estimation of Wound Healing Potential of Gnaphalium Hypoleucum Dc.

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