Screening of Probiotic Bacteria Candidates from Gastrointestinal Tract of Pacific White Shrimp Litopenaeus vannamei and their Effects on the Growth Performances

Abstract

This study aimed to get the probiotics bacteria from the gastrointestinal tract which had protease, amylase and lipase activities to improve growth performances of Pacific white shrimp (Litopenaeus vannamei). The probiotics were isolated from the gastrointestinal tract of Pacific white shrimp. The in vitro assays were carried out to isolate probiotics bacteria and selected the candidates based on digestive enzymes production (protease, amylase and lipase), resistance to gastric acid and bile salts, rapid bacterial growth phase, antagonistic activity against pathogenic bacteria, the attachment ability and pathogenicity to Pacific white shrimp. The selected isolates were then tested in vivo to observe their ability to improve growth performances of white shrimp. Five treatments with three replications consisted of control (feed without addition of any probiotic) and addition of four selected probiotics. Probiotics in a dose of 1% (v/w) feed were added every day for 60 days. Based on the results of in vitro selection, there were four selected isolates then applied to white shrimp including K9, M2, Z3 and S3. These four isolates have the highest ability in protein hydrolysis (K9), starch hydrolysis (M2), lipid hydrolysis (Z3) and protein, starch and lipid hydrolysis (S3). In addition, these isolates were also able to survive in acidic and alkaline condition, had antagonistic activity against pathogenic bacteria (V. harveyi), had the ability to adhere and not pathogenic for Pacific white shrimp. The feeding trial showed that isolate K9 showed the best results in improving protein digestibility (79.93±1.37%), protein retention (46.97±3.27%) and growth of Pacific white shrimp (1.09±0.01%).

Utilization of Ambon Banana (Musa acuminata) Peel Flour as a Prebiotic in the Nile tilapia (Oreochromis niloticus)

“…Fish digest nutrients in feed with the help of digestive enzymes, and then increase feed efficiency (Widanarni et al, 2015). Digestive enzyme activity and changes related to prebiotic inclusion in a feed show different results depending on fish species.…”