Screening of probiotic candidates in a simulated piglet small intestine <i>in vitro</i> model

Hansen, L.H.B.; Cieplak, Tomasz; Nielsen, Brian; Zhang, Y; Lauridsen, Charlotte; Canibe, Nuria

doi:10.1093/femsle/fnab045

Cited by 5 publications

(1 citation statement)

References 42 publications

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“…The excellent reproducibility of the CoMiniGut system to access the dynamic microbial communities as shown by high throughput sequencing allows multiple parallel runs that give freedom to perform different statistical analyses to strengthen the experimental findings (Wiese et al, 2018). The CoMiniGut system is new; still, it has been successfully used to study different dietary fibers (inulin, lactulose, and human milk oligosaccharide; Wiese et al, 2018), probiotics (Hansen et al, 2021), and new dietary substrates (Sagnelli et al, 2018; Wiese et al, 2021).…”

Section: Dynamic Modelmentioning

confidence: 99%

Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies

Singh

Son

Lee

et al. 2022

Biotech & Bioengineering

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Dietary studies play a crucial role in determining the health‐benefiting effects of most food substances, including prebiotics, probiotics, functional foods, and bioactive compounds. Such studies involve gastrointestinal digestion and colonic fermentation of dietary substances. In colonic fermentation, any digested food is further metabolized in the gut by the residing colonic microbiota, causing a shift in the gut microenvironment and production of various metabolites, such as short‐chain fatty acids. These diet‐induced shifts in the microbial community and metabolite production, which can be assessed through in vitro fermentation models using a donor's fecal microbiota, are well known to impact the health of the host. Although in vivo or animal experiments are the gold standard in dietary studies, recent advancements using different in vitro systems, like artificial colon (ARCOL), mini bioreactor array (MBRA), TNO in vitro model of the colon (TIM), Simulator of the Human Intestinal Microbial Ecosystem (SHIME), M‐SHIME, Copenhagen MiniGut, and Dynamic Gastrointestinal Simulator, make it easy to study the dietary impact in terms of the gut microbiota and metabolites. Such a continuous in vitro system can have multiple compartments corresponding to different parts of the colon, that is, proximal, transverse, and distal colon, making the findings physiologically more significant. Furthermore, postfermentation samples can be analyzed using metagenomic, metabolomic, quantitative‐polymerase chain reaction, and flow‐cytometry approaches. Moreover, studies have shown that in vitro results are in accordance with the in vivo findings, supporting their relevance in dietary studies and giving confidence that shifts in metabolites are only due to microbes. This review meticulously describes the recent advancements in various fermentation models and their relevance in dietary studies.

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Section: Dynamic Modelmentioning

confidence: 99%

Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies

Singh

Son

Lee

et al. 2022

Biotech & Bioengineering

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Editorial: microbial food and feed ingredients – functionality and health

Hansen

Nielsen

LaPointe

2021

FEMS Microbiology Letters

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Ceftiofur treatment of sows results in long-term alterations in the nasal microbiota of the offspring that can be ameliorated by inoculation of nasal colonizers

Blanco-Fuertes,

Sibila,

Franzo

et al. 2023

anim microbiome

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Background The nasal microbiota of the piglet is a reservoir for opportunistic pathogens that can cause polyserositis, such as Glaesserella parasuis, Mycoplasma hyorhinis or Streptococcus suis. Antibiotic treatment is a strategy to control these diseases, but it has a detrimental effect on the microbiota. We followed the piglets of 60 sows from birth to 8 weeks of age, to study the effect of ceftiofur on the nasal microbiota and the colonization by pathogens when the treatment was administered to sows or their litters. We also aimed to revert the effect of the antibiotic on the nasal microbiota by the inoculation at birth of nasal colonizers selected from healthy piglets. Nasal swabs were collected at birth, and at 7, 15, 21 and 49 days of age, and were used for pathogen detection by PCR and bacterial culture, 16S rRNA amplicon sequencing and whole shotgun metagenomics. Weights, clinical signs and production parameters were also recorded during the study. Results The composition of the nasal microbiota of piglets changed over time, with a clear increment of Clostridiales at the end of nursery. The administration of ceftiofur induced an unexpected temporary increase in alpha diversity at day 7 mainly due to colonization by environmental taxa. Ceftiofur had a longer impact on the nasal microbiota of piglets when administered to their sows before farrowing than directly to them. This effect was partially reverted by the inoculation of nasal colonizers to newborn piglets and was accompanied by a reduction in the number of animals showing clinical signs (mainly lameness). Both interventions altered the colonization pattern of different strains of the above pathogens. In addition, the prevalence of resistance genes increased over time in all the groups but was significantly higher at weaning when the antibiotic was administered to the sows. Also, ceftiofur treatment induced the selection of more beta-lactams resistance genes when it was administered directly to the piglets. Conclusions This study shed light on the effect of the ceftiofur treatment on the piglet nasal microbiota over time and demonstrated for the first time the possibility of modifying the piglets’ nasal microbiota by inoculating natural colonizers of the upper respiratory tract.

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Screening of probiotic candidates in a simulated piglet small intestine in vitro model

Cited by 5 publications

References 42 publications

Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies

Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies

Editorial: microbial food and feed ingredients – functionality and health

Ceftiofur treatment of sows results in long-term alterations in the nasal microbiota of the offspring that can be ameliorated by inoculation of nasal colonizers

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