Screening of pure synthetic coating substrates for induced pluripotent stem cells and iPSC-derived neuroepithelial progenitors with short peptide based integrin array

Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

“…Several studies in which stem cells are cultured on peptide-coated surfaces are summarized in Table S9 (ESI †). 89,92,98,108,129,150,173,174,[177][178][179][180][181][182][183][184][185][186][187] These investigations are discussed in more detail as follows.…”

“…8). 150 Acrylated poly-( l -lactide) (PLL) or poly(lactic- co -glycolic acid) (PLGA), can be prepared by partial hydrolysis of these biodegradable polyesters and subsequent reaction with acryloyl chloride. 130 The polymerization can be performed by photoirradiation, radical polymerization 126 or multithiol molecules such as PEG dithiol 255 and four-arm thiolated PEG.…”

“…Currently, at least 24 integrins are known, and human cells express 16 different integrins by a combination of different α chains and β chains. 150 Integrins, such as α2β1, α5β1, α6β1, and αvβ5, bind to specific ECM proteins. For example, integrin α6β1 mainly binds to laminin, whereas αvβ5 binds to vitronectin.…”

Design of chemically defined synthetic substrate surfaces for the in vitro maintenance of human pluripotent stem cells: A review

“… 80 In 2019, Jiang et al immobilized 16 kinds of integrin binding peptides onto the culture plates with the help of vinylsulfone functionalized PVA (PVA‐VS). The results showed that peptides binding to integrin α5β1 (no specified) could support cell adhesion and expansion of ZZUi011‐A hiPSCs in TeSR2 medium 81 . In 2021, Rramasubramanian et al developed bacterial to identify peptide ligands specific for the prominent stem cell surface receptor of α6β1‐integrin.…”

“…The results showed that peptides binding to integrin α5β1 (no specified) could support cell adhesion and expansion of ZZUi011-A hiPSCs in TeSR2 medium. 81 In 2021, Rramasubramanian et al developed bacterial to identify peptide ligands specific for the prominent stem cell surface receptor of α6β1-integrin. Triple-peptide composed of 40% Ag73 (syndecan binding motif, no specified), 40% bspRGD (15) (no specified), and 20% cyclic (7C-1) (α6β1-integrin binding motif, no specified) 11-MUA peptides could support the culture of H1 hESCs for a single passage (Figure 4C).…”