Screening of quinolone residues in pig muscle by planar chromatography

Abstract

A high-performance thin-layer chromatographic method based on solid-phase extraction has been developed for the qualitative determination of seven quinolones (enrofloxacin, ciprofloxacin, danofloxacin, norfloxacin, flumequine, oxolinic acid and nalidixic acid) in pork muscle. After preparation of the samples by extraction and clean-up by solid-phase extraction on reversed-phase cartridges, extracts were spotted and eluted on silica gel plates. The plate is first inspected under UV illumination at 312 nm, then sprayed with terbium chloride solution and again monitored under 312 nm UV. The method has been validated to a level of 15 lag kg -1 for enrofloxacin, ciprofloxacin, danofloxacin, norfloxacin and 5 lag kg -I for flumequin, oxolinic acid and nalidixic acid.

Separation and Detection of Certain ß-Lactam and Fluoroquinolone Antibiotic Drugs by Thin Layer Chromatography

“…These fluorinated quinolones are active against many Gram-positive and Gram-negative bacteria through inhibition of their DNA gyrase. 2 A literature survey has revealed various TLC methods for the selective separation and simultaneous determination of β-lactam [3][4][5][6][7][8][9][10] and fluoroquinolone [11][12][13][14][15][16] antibiotic drugs from biological fluids and pharmaceutical formulations. Wagman and Weinstein 17 reviewed TLC procedures useful for the identification and differentiation of numerous antibiotics, including penicillins.…”

Quantitative determination of quinolones residues in milk by HPLC-FLD

“…I campioni ufficiali, prelevati dall'autorità competente, sono analizzati in laboratori autorizzati attraverso metodi di screening e, in caso di positività, analizzati nuovamente con metodi di conferma. In letteratura sono descritti diversi metodi per la ricerca di residui di chinoloni nel latte; le tecniche microbiologiche non hanno sensibilità adeguata a soddisfare i requisiti richiesti dalla normativa vigente; le tecniche immunochimiche (Haasnoot et al, 2007;Wang et al, 2010), la cromatografia su strato sottile (TLC) (Chroma et al, 1999;Juhel-Gaugain et al, 1998), la gas cromatografia (GC) e la cromatografia liquida ad alte prestazioni (HPLC) accoppiata con rivelatore spettrofluorimetrico (FLD), a sequenza di diodi (DAD) o a spettrometria di massa (MS) (Yorke et al, 2000;Ramos et al, 2003;Zhao et al, 2007;Ho et al, 2004;Gigosos et al, 2000;Zhang et al, 2009;Bohm et al, 2009) sono invece maggiormente sensibili e in alcuni casi ido-nee ad un'inequivocabile identificazione degli analiti. Tuttavia questi metodi di solito comprendono solo una delle sottoclassi di chinoloni (derivati dell'acido piridoncarbossilico o fluorochinoloni con sostituente piperazinile al C-7).…”

The Visualizing Agents for Selected Quinolones and Fluoroquinolones

“…[9][10][11][12] Thin layer chromatography is also very popular for quinolones investigation using different stationery and mobile phases. [8,[13][14][15][16][17][18][19][20][21][22][23][24] There is a necessity for the chromatograms visualization in many cases. The Dragendorf and Forrest and Folin-Ciocalteu agents, as well as iron(III) chloride in hydrochloric acid, iodic agent, and phosphomolybdenic acid in sulfuric(VI) acid were applied for quinolones visualization until now.…”