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Virus enteritis of geese is one of the most important diseases of waterfowl. This disease causes high lethality in young goslings — up to 16,3–99,6% and significant economic damage to the industry. The infection is widespread in various regions of Russia. It is known that cell culture is an integral part of the laboratory diagnosis of goose parvovirus enteritis. For virus isolation, studying its biological properties, and its accumulation, cell cultures from the organs and tissues of goose embryos and their embryos are widely used. However, the cell cultures’ derivation from goose embryos depends on the seasonality of oviposition in this species of bird. Information on the cultivation of goose parvovirus in other species of cell culture is very limited. To determine the range of cell cultures suitable for virus replication, regardless of the season, is an urgent scientific problem that demands more extensive research. Cell cultures of heterologous species are successfully used for the cultivation of many viruses. In this regard, we studied the possibility of reproduction of the geese parvovirus in cell cultures of different tissues and species of origin: cattle, pigs, goats, sheep, monkeys, cats, geese, and hybrid cultures. It was found that heterologous cell cultures do not support goose parvovirus replication. Moreover, the pig-goose hybrid culture derived by the authors did not show sensitivity to the virus.
Virus enteritis of geese is one of the most important diseases of waterfowl. This disease causes high lethality in young goslings — up to 16,3–99,6% and significant economic damage to the industry. The infection is widespread in various regions of Russia. It is known that cell culture is an integral part of the laboratory diagnosis of goose parvovirus enteritis. For virus isolation, studying its biological properties, and its accumulation, cell cultures from the organs and tissues of goose embryos and their embryos are widely used. However, the cell cultures’ derivation from goose embryos depends on the seasonality of oviposition in this species of bird. Information on the cultivation of goose parvovirus in other species of cell culture is very limited. To determine the range of cell cultures suitable for virus replication, regardless of the season, is an urgent scientific problem that demands more extensive research. Cell cultures of heterologous species are successfully used for the cultivation of many viruses. In this regard, we studied the possibility of reproduction of the geese parvovirus in cell cultures of different tissues and species of origin: cattle, pigs, goats, sheep, monkeys, cats, geese, and hybrid cultures. It was found that heterologous cell cultures do not support goose parvovirus replication. Moreover, the pig-goose hybrid culture derived by the authors did not show sensitivity to the virus.
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