Screening of some medicinal plants from cameroon for <i>β</i>‐Lactamase inhibitory activity

Gangoué-Piéboji, Joseph; Baurin, Stéphane; Frère, Jean‐Marie; Ngassam, Pierre; Ngameni, Bathélémy; Azebaze, Anatole Guy Blaise; Pegnyemb, Dieudonné Emmanuel; Watchueng, Jean; Goffin, Colette; Galleni, Moreno

doi:10.1002/ptr.2001

Cited by 26 publications

(13 citation statements)

References 14 publications

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“…It is probable that some of these compounds, alone or in combination, are responsible for the observed antimicrobial properties as previously shown. In addition, previous studies showed that most of these plant extracts are beta-lactamase inhibitors [11]; therefore, active plant extracts had not only antimicrobial properties, but also anti-beta-lactamase activities. Moreover, some of the plant extracts showed good antifungal activities on yeast (D. picta), filamentous fungi (G. kola, G. lucida, B. micrantha) and all fungi (P. africana) (results not shown).…”

Section: Discussionmentioning

confidence: 99%

“…In an ongoing programme of research and development of traditional medicine in Cameroon focused on the screening of traditionally used Cameroonian plants for antimicrobial properties, we have reported antibacterial activities on Gram positive bacteria [10], and beta-lactamase inhibitory properties of some plant extracts [11]. The present study was initiated to evaluate the antimicrobial activity of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria.…”

Section: Introductionmentioning

confidence: 98%

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The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

Gangoué-Piéboji

Eze

Djintchui

et al. 2009

J Infect Dev Ctries

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Background: In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria. Methodology: The antimicrobial activities of plant extracts were evaluated against clinically proved beta-lactam-resistant bacteria (Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Serratia marcescens, Acinetobacter baumannii, Staphylococcus aureus and Enterococcus sp.) and reference strains of bacteria (Escherichia coli ATCC 35218, Enterobacter aerogenes ATCC 29751, E. aerogenes ATCC 13048, Pseudomonas aeruginosa ATCC 27853 and Enterococcus hirae ATCC 9790) by using disc-diffusion and agar-dilution assays. Results: The crude plant extracts demonstrated broad spectrum activity against all bacteria tested with inhibition zones in the range of 8-30 mm. The minimal inhibitory concentration (MIC) values of different plant extracts against the tested bacteria were found to range from ≤ 0.3 to ≥ 10 mg ml -1 . The most active plant extracts were from Dortenia picta and Bridelia micrantha (MIC: 1.25-10 mg ml -1 ) on beta-lactamresistant Gram-negative bacilli and the extracts from B. micrantha, Mallotus oppositifolius, Garcinia lucida, Garcinia. kola, Campylospermum densiflorum (leaves) and C. zenkeri (root) on beta-lactam-resistant Gram-positive cocci (MIC: ≤ 0.3-5 mg ml -1 ). Conclusion: Of the 17 plant extracts studied, seven showed good antimicrobial activity against the tested bacteria. The stem bark of B. micrantha and the leaves of D. picta were most active towards beta-lactamase producing Gram-negative bacilli. This study shows that medicinal plants could be sources of compounds which can be used to fight against beta-lactam resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

Gangoué-Piéboji

Eze

Djintchui

et al. 2009

J Infect Dev Ctries

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“…These probably also explain its reported aphrodisiac properties. In their efforts to find new bioactive β-lactamase inhibitors, Gangoué-Piéboji et al (2007; investigated 16 Cameroonian plants and found that, extracts from G. lucida, together with other species, presented interesting in vitro β-lactamase inhibition. The isolation and the structural elucidation of the active constituents of G. lucida could provide useful leads in the development of β-lactamase inhibitors.…”

Section: Resultsmentioning

confidence: 99%

<i>Garcinia lucida</i> Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development

Guedje¹,

Tadjouteu²,

Onana³

et al. 2017

J. App. Bioscience.

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Guedje et al., J. Appl. Biosci. 2017 Garcinia lucida Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development 10594Journal of Applied Biosciences 109: 10594-10608 ISSN 1997-5902 Garcinia lucida Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development ABSTRACT Objectives: This paper seeks to assess information on the identity, traditional uses, safety and efficacy of Garcinia lucida Vesque (Essok in Boulou and Ewondo local language), in order to highlight it potential as species to be registered in medicinal plant list and formularies needed for the development of monographs leading to local production of phytomedicines. Methodology and Results: Ethnobotanical survey was carried in the Bipindi-Akom II region (South Cameroon) with the help of interviews based on standardised questionnaires addressed to key-informants. Bark and seeds were the major parts used, highly appreciated due to its properties in preventing consumers from poison, diarrhoea and headaches. The most important therapeutic indications were additive to palm wine, antidote to poisoning, gastritis and snake bite. Other uses in treating included gynaecological pains and infections, sexual diseases and cancers. The bark was also believed by local people to act on the stability of chemical antibiotic drugs in pharmacy, while leaves were used as insect repellent against mosquitoes and cockroaches. Conclusions and application of findings: As an additive in palm wine processing, poisoning antidote, aphrodisiac and medicines, bark and seeds are widely used in the Bipindi-Lolodorf-Akom II area, in the Centre and South regions of Cameroon, in Gabon and Equatorial Guinea, in Central Africa and West Africa. This similarity of use across many regions is considered as important biological activity marker tools guiding plant parts or species selection in drug discovery and development using ethnopharmacological approach. Through the ethnobotanical and biological tests, assessed, accurate information is provided to substantiate safety and efficacy of G. lucida and to satisfy the requirements of official compendia such as pharmacopoeia. Further studies are needed to understand the effects of bark and seeds as additive to palm wine, as well as the interaction between bark and chemical antibiotic drugs.

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“…There are four main mechanisms used by bacteria to resist beta-1actamines molecules: i) enzymatic inactivation, ii) the modification of the cellular target of the antibacterial agent, iii) the cellular efflux and, iv) the decrease or absence of penetration of the antibiotic in the bacteria. The combination of these mechanisms in a bacterium makes its multi-resistant to many antibiotics (Gangoue-Piéboji et al, 2004). Similarly, several cases of fungi resistant to conventional antifungal agents have been reported.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical screening and biological activities of Garcinia kola (bark, leaves and seeds) collected in Benin

Durand¹,

Farid²,

Adjanohoun³

et al. 2015

Afr. J. Microbiol. Res.

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Garcinia kola is a tropical plant which grows in moist forest. It seeds are edible and are consumed for their multiple property. This study aimed at making phytochemical screening and evaluating some biological activities of G. kola organs (bark, leaves and seeds) extracts. The selected organs powdered were used for phytochemical screening and extractions. The antibacterial activity was tested on 10 references strains and 28 meat isolated Staphylococcus strains by agar perforation method. The antifungal activity of three fungal strains was determined on the potato-dextrose agar medium mixed with the tested extract. The antioxidant activity was determined by both DPPH and ABTS methods. The results reveal that 40% of studied secondary metabolites were present in the seeds against 28.57% in the leaves as the bark. The reference and meat isolated strains inhibitory diameter ranged from 28 ± 2.3 mm (S. haemolyticcus) to 7.5 ± 0.70 mm (E. coli). All extracts had a minimum inhibitory concentration (MIC) lower than 20 mg/ml, while the minimum bactericidal concentration (MBC) was ≥ 20 mg/ml for some tested extracts. The lowest MIC and MBC was 0.039 mg/ml with C. albicans. The fungal strains susceptibility varied (p  0.001) depending on extracts. The seeds ethyl acetate extract displays the better antioxidant activity with DPPH (25.03 ± 3.64 μg/μl) and ABTS (22.99 ± 1.34 µmol EqAA/g) methods. The LD 50 of the extracts was greater than 0.1 mg/ml and not exhibiting toxicity. The leaves extract had the best bactericidal effects while the seeds extract presented better antifungal and antioxidant activity.

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Screening of some medicinal plants from cameroon for β‐Lactamase inhibitory activity

Cited by 26 publications

References 14 publications

The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

<i>Garcinia lucida</i> Vesque (Clusiaceae): from traditional uses to pharmacopeic monograph for an emerging local plant-based drug development

Phytochemical screening and biological activities of Garcinia kola (bark, leaves and seeds) collected in Benin

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