Screening of the antibacterial effects of a variety of essential oils on respiratory tract pathogens, using a modified dilution assay method

Inouye, Shigeharu; Yamaguchi, Hideyo; Takizawa, Toshio

doi:10.1007/s101560170022

Cited by 181 publications

(106 citation statements)

References 16 publications

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“…The antimicrobial effect of cinnamaldehyde is related to its ability to bind to proteins preventing the action of amino acid decarboxylases 17 . It has been demonstrated that Cinnamomum zeylanicum oil has an inhibitory effect against a large variety of pathogenic microorganisms, including Haemophilus influenzae, Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus and Escherichia coli 18 . Strong antimicrobial activity (MIC 90 = MBC 90 = 600 μg/mL) was also determined for carvacrol, a phenolic compound isolated from leaves and flowers of Thymus vulgaris L. and Origanum vulgare.…”

Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against Mycoplasma hominis clinical isolates

Sleha¹,

Mosio²,

Vydržalová³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. The aim of this study was to evaluate the antimicrobial effects of five natural substances against 50 clinical isolates of Mycoplasma hominis. Methods and Results. The in vitro activity of selected natural compounds, cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene, was investigated against 50 M. hominis isolates cultivated from cervical swabs by the broth dilution method. All showed valuable antimicrobial activity against the tested isolates. Oil from the bark of Cinnamomum zeylanicum (MBC 90 = 500 μg/mL) however was found to be the most effective. Carvacrol (MBC 90 = 600 μg/mL) and eugenol (MBC 90 = 1000 μg/mL) also possessed strong antimycoplasmal activity. Conclusions. The results indicate that cinnamon bark oil, carvacrol and eugenol have strong antimycoplasmal activity and the potential for use as antimicrobial agents in the treatment of mycoplasmal infections.

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Section: Discussionmentioning

confidence: 99%

In vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against Mycoplasma hominis clinical isolates

Sleha¹,

Mosio²,

Vydržalová³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…Inhibitory effects of some essential oils against respiratory track bacteria have been investigated (Inouye et al 2001;Fabio et al 2007). In South Korea, conifer species in the plant families Pinaceae (e.g., Pinus koraiensis, Pius densiflora, Larix kaempferi, Abies holophylla) and Cupressaceae (e.g., Chamaecyparis obtusa, Platycladus orientalis, Chamaecyparis pisifera) are widely distributed (Shannon, 1956).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Essential Oil from Abies holophylla against Respiratory Tract Bacteria

Lee¹,

Kim²,

Park³

et al. 2014

Journal of the Korean Wood Science and Technology

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We extracted essential oils from four species (Pinus densiflora, Larix kaempferi, Pinus koraiensis, and Abies holophylla) in the family Pinaceae to investigate their antibacterial activities against respiratory tract bacteria (Klebsiella pneumoniae, Haemophillus influenzae, Streptococcus pyogenes, Streptococcus pneumonia, and Neisseria meningitides). Among the tested oils, that from A. holophylla was showed strong activity based on disc diffusion and broth medium dilution (minimum inhibitory concentration, MIC) assays. Qualitative analysis of A. holophylla oil was carried out by GC-MS; α -pinene, camphene, β -pinene, 3-carene, limonene, bornyl acetate, borneol, β -caryophyllene, α -caryophyllene, caryophyllene oxide, and α -bisabolol were identified as its major constituents. Fractionation by silica gel chromatography was performed to analyze the active constituents of the crude oil. In particular, one fraction containing caryophyllene oxide as the major constituent showed stronger antibacterial activity than the crude oil of A. holophylla. Growth rates of bacterial strains exposed to fraction D were explored by optical density (OD600) measurements while morphology was examined by optical microscopy observations (× 1000). OD600 of K. pneumoniae decreased from 0.2582 to 0.005 in response to treatment with fraction D at a MIC value of 0.31 μ ℓ /mℓ .

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“…However, when extracts are applied in a concentrated form as in the present study, the delivery of these hydrophobic compounds to bacterial cells may be reduced. An uneven distribution and accessibility of terpenes to bacterial cells may be one of the reasons for the relatively low bactericidal activity (Inouye et al 2001), where the insolubility of the constituents in the culture medium may change the effectiveness of the extract besides chemical composition of plant material. Romeo et al (2008) showed the antimicrobial efficacy of essential oil of A. triphylla in an experiment using a liquid medium.…”

Section: Resultsmentioning

confidence: 99%

Chemical composition and antibacterial activity of Aloysia triphylla (L'Hérit) Britton extracts obtained by pressurized CO2 extraction

Parodi

Vargas

Krewer

et al. 2013

Braz. arch. biol. technol.

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show abstract

Screening of the antibacterial effects of a variety of essential oils on respiratory tract pathogens, using a modified dilution assay method

Cited by 181 publications

References 16 publications

In vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against Mycoplasma hominis clinical isolates

In vitro antimicrobial activities of cinnamon bark oil, anethole, carvacrol, eugenol and guaiazulene against Mycoplasma hominis clinical isolates

Antibacterial Activity of Essential Oil from Abies holophylla against Respiratory Tract Bacteria

Chemical composition and antibacterial activity of Aloysia triphylla (L'Hérit) Britton extracts obtained by pressurized CO2 extraction

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