Screening of the genotypes of bread wheat (Triticum aestivum L.) by the allelic variants of Waxy genes and HMW glutenin subunits

Abstract

Screening genotypes of wheat by allelic variants of <em>Waxy</em> and <em>HMW-GS</em> genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of <em>Triticum aestivum</em> L. with valuable alleles of <em>Waxy</em> and <em>HMW-GS</em> genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of <em>Waxy</em> and <em>HMW-GS</em> genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (<em>Wx-A1b</em> and <em>Wx-B1b</em>) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of <em>HMW-GS</em>, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify <em>Waxy</em> and <em>HMW-GS</em> alleles are effective methods of evaluating their allelic polymorphism.

Development of the new waxy winter wheat cultivars Eldija and Sarta

“…The development of waxy wheat in Europe was recently reported in Germany (Bundessortenamt 2017), Italy (Caramanico et al 2018) and France (Debiton et al 2010;SECOBRA Recherches 2021). The breeding of waxy wheat has been taken up in Ukraine (Iorgachova et al 2018) and Russia (Vafin et al 2018) as well.…”

Creation and study of partial waxy wheat lines adapted for the Middle Volga region of Russia

“…Key words: wheat; waxy; non-functional alleles; disease resistance; productivity. 4F-c: 5'-CCCCCAAGAGCAACTACCAGT-3' Wx-B2R: 5'-CGTTGACGATGCCGGTGTTG-3' (Vafin et al, 2015(Vafin et al, , 2018 Wx-B1…”

Nanopore Amplicon Sequencing Allows Rapid Identification of Glutenin Allelic Variants in a Wheat Collection

“…However, these techniques are timeconsuming, require high-precision equipment, and cannot be scaled to evaluate breeding material. Allelic variants of HMW-GSs are also detected by DNA markers, including KASP markers, enabling the differentiation of high-molecular-weight glutenin subunits, even when they have similar molecular weights [7,22]. For example, KASP markers have been used to accurately detect alleles of the Glu-1 locus by identifying single nucleotide polymorphisms (SNPs) in gene sequences.…”