Screening genotypes of wheat by allelic variants of <em>Waxy</em> and <em>HMW-GS</em> genes is a constitutional unit of marker-assisted selection of varieties with high values of desirable properties for flour-baking and technological attributes of the grain. The aim of the study was to produce a molecular screening of samples of spring soft wheat of local selection lines for the detection of genotypes of <em>Triticum aestivum</em> L. with valuable alleles of <em>Waxy</em> and <em>HMW-GS</em> genes. Seventy samples of wheat were subjected to DNA testing for the identification of genotypes with alleles of <em>Waxy</em> and <em>HMW-GS</em> genes. Molecular screening of wheat samples with the selected systems of molecular marking of the allelic variants of the analyzed genes made it possible to detect three partially Waxy lines with a combination of two null-alleles (<em>Wx-A1b</em> and <em>Wx-B1b</em>) in the study collection, as well as 33 plants with an economically-valuable combination of Ax2*/5+10 subunits of <em>HMW-GS</em>, the genotypes of which are considered as starting material for further selection work on the creation of spring soft wheat cultivars with high quality indicators of grain. The approaches applied in the study to identify <em>Waxy</em> and <em>HMW-GS</em> alleles are effective methods of evaluating their allelic polymorphism.
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