Screening, selection and development of Bacillus subtilis apr-IBL04 for hyper production of macromolecule alkaline protease

Abstract

Bacillus subtilis microbe is commonly found in soil and produces proteases on nitrogen and carbon-containing sources and increases the fertility rate by degrading nitrogenous organic materials. The present study was aimed to develop hyper producing mutant strain of B. subtilis for the production of proteases, to improve the process variables by the response surface methodology (RSM) under central composite design (CCD) and the production of protease by the particular mutant strain in a liquid state fermentation media. The mutation of the strain was carried out using ethidium bromide. Pure B. subtilis strain was collected and screened for hyper-production of protease. The production of protease by mutant B. subtilis strain was optimized by varying temperature, inoculum size, pH and incubation time under liquid state fermentation. The CCD model were found to be reliable with r 2 of 0.999. The maximum enzyme activity of B. subtilis IBL-04 mutant with 3 mL/100 mL inoculum size, 72 h fermentation time, pH 8, and 45 °C temperature was developed with enzyme activity 631.09 U/mL, indicates 1–7-fold increase in enzyme activity than the parent strain having 82.32 U/mL activity. These characteristics render its potential use in industries for pharmaceutical and dairy formulation.

Effect of fermentation on structural properties and antioxidant activity of wheat gluten by Bacillus subtilis

“…The color of the bands in the range of 17-25 kDa gradually became darker, and the color of the bands in the range of 100, 70, 50, 35 kDa gradually became lighter and disappeared. Bacillus subtilis can synthesize extracellular protease itself, under the action of enzymes, gluten gradually degrades into small-molecule polypeptides, these small-molecule polypeptides have a variety of biological activities (44). Padhi et al (11) fermented soybeans with Bacillus subtilis and identified 16 peptides by LC-MS/MS analysis, including the common peptide PFPIPFPIPIPLP and the characteristic peptide IPFPPIPFLIP, which had a significant effect on the inhibitory activity of angiotensinconverting enzyme (ACE).…”

Proteases E Suas Aplicações Biotecnológicas Nas Indústrias Alimentícias

“…A produção de compostos bioativos, como as proteases, por microrganismos se dá através de processos fermentativos em que geralmente há apenas a necessidade de um substrato e condições favoráveis ao crescimento do organismo e a produção de biomoléculas, o que pode facilmente ser controlado durante a fermentação. Ao realizar uma breve pesquisa em bancos de dados científicos, foi observado que cientistas já produziram proteases a partir de fungos, bactérias e cianobactérias (ELLEUCH et al, 2021;OSMOLOVSKIY et al, 2021;SHAFIQUE et al, 2021). Alguns organismos produtores de proteases pertencentes as três fontes supracitadas, estão descritas na Tabela 02.…”

"Isolation and Characterization of Detergent Compatible Alkaline Protease Producing Bacillus Subtilis APO-1"

“…amount of alkaline proteases having significant proteolytic activity, stability at high temperature and wide range of pH. Number of studies has been reported on alkaline protease production by Bacillus like Bacillus cereus [6], Bacillus infantis [7], Bacillus subtilis [8], Bacillus pumilus [9] etc. Microbial proteases, especially from Bacillus subtilis are the most widely exploited industrial enzymes [10].…”