Screening wheat genotypes for resistance to wheat blast disease in the vegetative and reproductive stages

Rocha, João Romero do Amaral Santos de Carvalho; Paula, Igor Gonçalves de; Gloria, Hilda Barbosa; Pimentel, Adérico Júnior Badaró; Ribeiro, Guilherme; Souza, Moacil Alves de

doi:10.1007/s10681-019-2382-9

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…Group 1 will combine the TBIO Aton, TBIO Sintonia, TBIO Duque, TBIO Sossego, TBIO Ponteiro, and CD 1303 parents owing to their high GY and DN estimates. Parents that are resistant to wheat blast disease are essential, as this is a major disease of wheat production systems in central Brazil (Rocha et al 2019). However, these genotypes had the disadvantage of longer cycles (66.16 -69.30 days) and PH values slightly higher than those usually sought by breeders, ranging from 85.30 (TBIO Aton) to 94.35 cm (TBIO Sintonia).…”

Section: Resultsmentioning

confidence: 99%

Choosing parent tropical wheat genotypes through genetic dissimilarity based on REML/BLUP

Casagrande

Mezzomo

Cruz

et al. 2020

Crop Breed. Appl. Biotechnol.

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Section: Resultsmentioning

confidence: 99%

Choosing parent tropical wheat genotypes through genetic dissimilarity based on REML/BLUP

Casagrande

Mezzomo

Cruz

et al. 2020

Crop Breed. Appl. Biotechnol.

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“…This suggests that selecting for the BR 18 haplotype within the QTL regions on 2A, 4B and 5A may provide a basal level of resistance to wheat blast at the heading stage, which could be further enhanced by introducing race-specific resistances. Some studies have observed that under certain conditions BR 18 may display moderate susceptibility [ 24 , 41 ]. In this study, BR 18 conferred the resistant allele for six of the nine QTL identified, demonstrating that BR 18 has both positive and negative alleles associated with blast resistance.…”

Section: Discussionmentioning

confidence: 99%

Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena

et al. 2020

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Background Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat ( Triticum aestivum L.) production. Few blast resistance ( R ) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. Results BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F 6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18-Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terena-like haplotype for more than one of the identified heading stage QTL. Conclusions This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates marker-assisted selection to develop new varieties for cultivation in regions at risk from this emerging disease.

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“…(Rajiv 2017;Sadat and Choi 2017;Cruz and Valent 2017;Mottaleb et al 2018Mottaleb et al , 2019Ceresini et al 2018Ceresini et al , 2019Islam et al , 2020Kamoun et al 2019;Tembo 2019;Yesmin et al 2020;Mbinda and Masaki 2021, Singh et al 2021, Tosa 2021. While some aspects have been given more attention, such as the taxonomy and population genetics (Couch and Kohn 2002;Tanaka et al 2009;Luo and Zhang 2013;Maciel et al 2014;Murata et al 2014;Klaubauf et al 2014;Gladieux et al 2015Gladieux et al , 2018Castroagudín et al 2016;Valent et al 2019), chemical control (Kim et al 2003;Cruz et al 2011Cruz et al , 2019Pagani et al 2014;Rocha et al 2014;Castroagudín et al 2015;Oliveira et al 2015;Dorigan et al 2019;D'Ávila et al 2021;Ascari et al 2021) and host genetic resistance (Cruz et al 2016b;Anh et al 2018;Rocha et al 2019;Cruppe et al 2020;Inoue et al 2021;Dianese et al 2021;…”

Section: Economical and Scientific Importancementioning

confidence: 99%

“…Host genetic resistance is an important tool to integrate into disease management, but the progress in breeding for blast resistance has been challenged by a disease-conducive environment, especially in the tropics, as well as high variability in the fungal pathogen population (Coelho et al 2016;Rocha et al 2019;Gupta et al 2021;Duan et al 2021). Few wheat cultivars have been classified as resistant, or moderately resistant, to wheat blast, and in general most of them behave as moderately or fully susceptible to blast especially under disease-inductive weather conditions (Prestes et al 2007;Cruz et al 2012;Gomes et al 2019; Comissão Brasileira de Pesquisa de Trigo e Triticale 2020; Cruppe et al 2020;Goddard et al 2020;Dianese et al 2021;Roy et al 2021, Singh et al 2021.…”

Section: Wheat Blast Managementmentioning

confidence: 99%

“…Specific environmental conditions such as temperature directly affect the expression of genetic resistance, and in other cases, races of the pathogen contribute to overcoming the resistance (Maciel et al 2014;Cruz et al 2016b). Breeding efforts are challenged by the high genetic diversity of the populations, reinforcing the need to unveil both the genetic variability of the regional blast pathogen population (Rocha et al 2019;Asuke et al 2021) or a pandemic population recently reported in Asian wheat fields (Tembo et al 2020(Tembo et al , 2021Croll 2021;Roy et al 2021).…”

Section: Wheat Blast Managementmentioning

confidence: 99%

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Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

Ascari¹,

Ponte²

2021

Preprint

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The blast disease of Poaceae is caused by a large species complex, among which P. oryzae is composed of several host-specialized lineages. The Pyricularia oryzae Triticum pathotype (PoT) causes the blast disease in wheat, but is also capable of infecting other grasses, which may serve as an inoculum reservoir for epidemics in wheat. In Brazil, severe wheat blast epidemics are most common in the Cerrado region. The dominant hypothesis is that signal grass (Urochloa sp.) and other gramineous plants harbor the wheat blast pathogen, thus serving as a major reservoir of inoculum for epidemics in wheat. A two-year survey of the Pyricularia blast pathogens was conducted in both wheat and non-wheat areas as well as prior (February) and during (May) the wheat growing season in Minas Gerais. A total of 1,368 plant samples representative of 31 Poaceae species, including wheat, were collected and inspected for the presence of blast symptoms. During the isolations, 932 isolates were obtained, being one fourth obtained from gramineous plants. A subset of 572 isolates was selected for identification at the species level based on portions of the CH7-BAC9 gene sequences. Most of the isolates (n = 494) were P. oryzae, within which 68% were PoT and 32% non-PoT based on two PCR assays targeting (MoT3 and C17 PCR assays). The PoT lineage was found predominantly (97%) in wheat and rarely in the other hosts, even nearby wheat fields (2.1%), as well as at longer distances from wheat regions (0.1%). The blast pathogen population isolated from signal grass grouped in different clades from PoT, and therefore referred to Urochloa lineage (PoU). A series of cross-inoculation greenhouse experiments was conducted using wheat (cv. BRS Guamirim and BR 18-Terena) and signal grass (cv. Marandu) as host and 14 PoT and six PoU isolates as pathogen factor. In the first leaf-inoculation experiment, results showed a significant interaction between host and pathogen; PoT was strongly/weakly aggressive towards wheat/signal grass and PoU was strongly/weakly aggressive towards signal grass/wheat. In inoculated wheat heads, PoT was more aggressive (>91% infected spikelets) than PoU (52% infected spikelets). In a third experiment, four signal grass cultivars (Marandu, Basilisk, Piatã, and Xaraés) were inoculated with the same set of 20 isolates. Similarly, signal grass cultivars were generally more susceptible to PoU than PoT. Severity induced by PoU was twice (7.7% severity) as high as PoT (3.8%) and so was the number of conidia/leaf produced by PoU (47,500) and PoT (23,200). Two groups of signal grass cultivars were formed, the most susceptible composed of Marandu and Basilisk and the least susceptible composed of Piatã and Xaraés. Results of our study confirm the host-specialization and the shaping of the blast populations according to the host. We further suggest that grasses in general, especially signal grass, may not play a major role as an inoculum reservoir for PoT, as it harbors mainly the PoU population. However, due to the large extent of pasture-growing regions and cross-infection ability in wheat, signal grass may harbor amounts of PoT inoculum that are sufficient for initiating leaf and head blast epidemics in wheat blast in Minas Gerais state.

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Screening wheat genotypes for resistance to wheat blast disease in the vegetative and reproductive stages

Cited by 8 publications

References 19 publications

Choosing parent tropical wheat genotypes through genetic dissimilarity based on REML/BLUP

Choosing parent tropical wheat genotypes through genetic dissimilarity based on REML/BLUP

Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena

Taxonomic and pathogenic diversity of the blast pathogen populations infecting wheat and grasses in Minas Gerais

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