Screening wild sunflower species and F1 interspecific hybrids for resistance to broomrape

Abstract

Broomrape has become one of the most important threats to the cultivated sunflower. New sources of resistance genes are needed to maintain sunflower production. The objective of this research was to evaluate accessions of wild sunflower species and their F 1 interspecific hybrids with cultivated sunflower for resistance to race E. Plant material consisted of 6 accessions of perennial species and 14 F 1 accessions between perennial species and cultivated sunflower as well as 42 accessions of annual species. Cultivated line JM8 was used as the sensitive check. Accessions were screened in the greenhouse in the season of 2008/09 and in a field trial during 2009. Plants were grown in the infested soil and evaluated for reaction at the end of the vegetation. The cultivated check was susceptible in the greenhouse and in the field trial. The annual species showed varying resistance with H. annuus and H. argophyllus as the most sensitive with an average of 6.6 and 8.5 broomrape plants per sunflower plant. Only 2 of the 7 H. petiolaris accessions were susceptible. H. neglectus performed well in the greenhouse with only one broomrape plant infecting one plant of the four tested accessions, but accession NEG1363 was infected in the field trial leaving NEG457 and 1183 as resistant. All accessions of perennial species except for an F 1 hybrid of DEC B and F 1 RIG 707 showed complete resistance. New potential sources of broomrape resistance genes have been found among wild species and their interspecific hybrids.

Evaluation of resistance of new sunflower hybrids to broomrape in the breeding programs in Novi Sad

“…Variant of Pachenkov's (1973) method used in the quarantine chamber for testing sunflower breeding material in the greenhouse. The broomrape attack was assessed by uprooting and careful observation of root system for broomrape nodules and stalks Terzić et al (2010). Evaluation of the broomrape attack of selected hybrids in greenhouse conditions (Table 1).…”

“…Pancenko's (1973) method are used in all major breeding centers around the word. At the Institute of Field and Vegetable Crops a modification of Pachenkov's method is applied, the quarantine chamber for testing sunflower breeding material in greenhouse was done in plastic buckets (Dozet et al,1999), plastic tubes (Maširević and Medić-Pap, 2009 ) and in pots Terzić et al (2010).…”

Screening of wild helianthus species for resistance to high virulent Orobanche cumana Wallr., affecting sunflower in the Rostov region of the Russian Federation

“…However, plants of this species are completely resistant to broomrape from Yeysk area of Krasnodar region, so are the samples of H. debilis and H. praecox (Table 1). In researches of Terzić et al (2010) it is shown that in Serbia these species show resistance to race E of O. cumana. That corresponds to our data as well.…”

“…Many researches from other countries had reported earlier on the resistance of some annual and perennial wild species of sunflower to different races of O. cumana (Ruso et al, 1996;Christov, 1996;Christov et al, 2009;Fernandez-Martinez et al, 2000;Terzić et al, 2010). It has been proved that the wild species of Helianthus are the main source of the genes that control the resistance to new virulent races of broomrape.…”

10.5937/ratpov50-4648 = Resistance evaluation of different inbreds and hybrids of sunflower to broomrape (Orobanche cumana wallr.)

“…Due to the narrow genetic basis of cultivated sunflower for finding genes for the known races of the parasite research on wild sunflower species has been performed (Dozet & Marinković 1998, Fernadez-Martinez et al 2004, Jan 2004, Terzić et al 2010.…”