Scutellarein ameliorates tongue cancer cells via mitochondria

Jing, Guangping; Zheng, Junhua; Wang, Xueyong; Li, Haixia; Jiao, Xiaohui

doi:10.2478/s11536-013-0269-z

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Recent studies have showed that baicalein and scutellarein can induce apoptosis of cancer cells. 23,24) The mechanism of apoptosis induced by baicalein and scutellarein has been widely reported. Baicalein and scutellarein effectively up-regulated the expression of mitochondrial Bax and caspase-3, down-regulated the expression of Bcl-2, and decreased the mitochondrial transmembrane potential (ΔΨm).…”

Section: Discussionmentioning

confidence: 99%

“…Baicalein and scutellarein effectively up-regulated the expression of mitochondrial Bax and caspase-3, down-regulated the expression of Bcl-2, and decreased the mitochondrial transmembrane potential (ΔΨm). [23][24][25] These data suggest that the mechanism by which baicalein and scutellarein induce apoptosis involves the mitochondrial signaling pathway. It has been reported that luteolin, apigenin and chrysin in addition to inducing apoptosis through the ubiquitin-proteasome pathway, also can induce apoptosis through the mitochondrial signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Hydroxy Groups in the A-Ring on the Anti-proteasome Activity of Flavone

Nakamura

Yang

Sato

et al. 2015

Biological & Pharmaceutical Bulletin

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The ubiquitin-proteasome pathway plays an important role in regulating apoptosis and the cell cycle. Recently, proteasome inhibitors have been shown to have antitumor effects and have been used in anticancer therapy for several cancers such as multiple myeloma. Although some flavones, such as apigenin, chrysin and luteolin, have a specific role in the inhibition of proteasome activity and induced apoptosis in some reports, these findings did not address all flavone types. To further investigate the proteasome-inhibitory mechanism of flavonoids, we examined the inhibitory activity of 5,6,7-trihydroxyflavone, baicalein and 5,6,7,4′-tetrahydroxyflavone, scutellarein on extracted proteasomes from mice and cancer cells. Unlike the other flavones, baicalein and scutellarein did not inhibit proteasome activity or accumulate levels of ubiquitinated proteins. These results indicate that flavones with hydroxy groups at positions 5, 6 and 7 of the A-ring lack the antiproteasome function. Key words ubiquitin; flavonoid; proteasome; inhibitorThe ubiquitin-proteasome pathway has an important role in regulating the cell cycle and apoptosis.1) This pathway involves two steps: (i) conjugation of multiple ubiquitin molecules to the target protein, and (ii) degradation of the ubiquitin-tagged protein by the 26S proteasome. The latter is a multi-subunit protease complex comprising a 20S core associated with two 19S regulatory caps. The 20S core particle is cylindrical and consists of two outer heptameric rings of α-subunits and two inner heptameric rings of β-subunits. The three main catalytic activities of proteasomes are associated with the 20S core. They are peptidylglutamyl peptide hydrolyzing (PGPH) activity (mediated by the β1 subunit), trypsin-like (T-L) activity (mediated by the β2 subunit), and chymotrypsin-like (CT-L) activity (mediated by the β5 subunit). 2)Flavonoids are a group of polyphenolic substances that are widely distributed in plants, fruits vegetables, tea and wine. Flavonoids reportedly have pharmacological effects that include antioxidant, antitumor, antiviral and anti-inflammatory activities. [3][4][5][6][7][8] Recent studies have shown that certain flavonoids can inhibit proteasome activity and induce apoptosis in tumor cells, which suggests that flavonoids have potential as a new type of anticancer drugs.9,10) However, not all flavonoids are strong inhibitors of the proteasome. Their proteasomal inhibitiory potency is dependent upon their chemical structure. Some flavones (e.g., luteolin, apigenin) are markedly more potent than other flavonoids such as flavonols (e.g., kaempferol), or flavanones (e.g., naringenin and eriodictyol).11) Flavonoid glycoside is one type of flavonoids, and some flavonoid glycosides, such as baicalin and scutellarin, reportedly affect the inhibition of proteasomes. 12) Flavones have been shown to have structure-activity relationships; the apoptosis-inducing potencies in tumor cells and inhibitory potencies in 20S purified proteasome were luteolin> apigenin>chrysin.11) Furthermor...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Hydroxy Groups in the A-Ring on the Anti-proteasome Activity of Flavone

Nakamura

Yang

Sato

et al. 2015

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

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“…[11,12] Several studies have proposed that the anticancer mechanism of scutellarein involves a reactive oxygen species (ROS)-mediated mitochondriadependent pathway. [11,13,14] However, whether scutellarein has an effect in OC has not been thoroughly evaluated.…”

Section: Introductionmentioning

confidence: 99%

Scutellarein induces apoptosis and inhibits proliferation, migration, and invasion in ovarian cancer via inhibition of EZH2/FOXO1 signaling

Xiao

Zheng

et al. 2021

J Biochem & Molecular Tox

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Scutellarein, a flavone found in the perennial herb Scutellaria baicalensis, has antitumorigenic activity in multiple human cancers. However, whether scutellarein can attenuate ovarian cancer (OC) is unclear. This study investigated the effects of scutellarein in OC. In vitro cell viability was assessed using MTT assay whereas proliferation was assessed using 5-ethynyl-2′-deoxyuridine and colony formation assays. Cell apoptosis was detected by an Annexin V-fluorescein isothiocyanate/ propidium iodide assay. Wound-healing and Transwell assays were used to determine cell migration and invasion. The differential expression of enhancer of zeste homolog 2 (EZH2) and forkhead box protein O1 (FOXO1) was measured by Quantitative real-time PCR and western blot analysis. We found that scutellarein inhibited viability, migration, invasion of A2780 and SKOV-3 cells, and reduced the expression of EZH2 in OC cells. In addition, FOXO1 was downregulated in OC tissues and cells and negatively regulated by EZH2. Also, scutellarein inhibited tumor growth and metastasis in vivo. In conclusion, scutellarein alleviates OC by the regulation of EZH2/FOXO1 signaling.

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Scutellarein Apoptosis Mediated by Mitochondria in Oral Squamous Cell Carcinomas

Birundadevi

Sivashankar

Mathukumar

2022

Biomedical and Biotechnology Research Journal (BBRJ)

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Background: The growth of several cancers can be inhibited by naturally occurring medicinal plants. A flavone called Scutellarein found in the perennial herb Scutellaria lateriflora does have a wide range of biological functions. Scutellarein was studied to determine whether it could induce apoptosis and cause cytotoxicity. Methods: On oral squamous cell carcinoma KB cell lines, Scutellarein's cytotoxic activity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Scutellarein was added to KB cells at concentrations ranging from 25 to 125 g/mL for 24 h. The real-time polymerase chain reaction was also used to investigate apoptotic induction potential in Scutellarein-incubated KB cell lines by analyzing Bcl-2 protein and gene expression. Results: In KB-cell lines treated with scutellarein, cytotoxicity and anticancer effects were observed, as well as inhibit the growth of cancer cells. In comparison to the cells not treated with scutellarein, KB cells that had been exposed to scutellarein displayed reduced Bcl-2 expression. Conclusion: KB cells were treated with scutellarein to induce apoptosis, suggesting its potential as a chemo preventative agent. This activity appears to be mediated through the modulation of Bcl-2, a cytotoxic gene.

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Scutellarein ameliorates tongue cancer cells via mitochondria

Cited by 3 publications

References 31 publications

Effects of Hydroxy Groups in the A-Ring on the Anti-proteasome Activity of Flavone

Effects of Hydroxy Groups in the A-Ring on the Anti-proteasome Activity of Flavone

Scutellarein induces apoptosis and inhibits proliferation, migration, and invasion in ovarian cancer via inhibition of EZH2/FOXO1 signaling

Scutellarein Apoptosis Mediated by Mitochondria in Oral Squamous Cell Carcinomas

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