SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells

Li, Linli; Liu, Xifeng; Gaihre, Bipin; Park, Sungjo; Li, Yong; Terzic, André; Lu, Lichun

doi:10.1155/2021/1938819

Cited by 7 publications

(5 citation statements)

References 31 publications

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“…The chemotactic activity of CXCL12 for MSCs has been widely exploited to recruit MSCs to a specific lesion [49]. The migration of cultured MSCs or endogenous MSCs into SDF-1α-loaded hydrogels or scaffolds was demonstrated in vitro and in vivo [22,23]. A RARRES2-loaded 10% HA-silk scaffold that was implanted into a rat tibial defect failed to recruit endogenous cells into the scaffold.…”

Section: Discussionmentioning

confidence: 99%

“…The CXCL12 (SDF-1)-CXCR4 axis has been reported to stimulate MSC migration to injury sites including bone fractures [21]. MSCs or endogenous cells can also be recruited into CXCL12-loaded 3-D scaffolds in vitro and in vivo [22,23]. In addition, the chemerin (RARRES2 gene product)-CMKLR1 (chemokine-like receptor 1) axis has been reported to activate MSC migration to cancer tissue and damaged liver lesions [24,25].…”

Section: Introductionmentioning

confidence: 99%

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Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts

Cho

Lee

Park

et al. 2022

CIMB

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Mesenchymal stem cells (MSCs) have been widely applied to the regeneration of damaged tissue and the modulation of immune response. The purity of MSC preparation and the delivery of MSCs to a target region are critical factors for success in therapeutic application. In order to define the molecular identity of an MSC, the gene expression pattern of a human bone marrow-derived mesenchymal stem cell (hBMSC) was compared with that of a human embryonic fibroblast (hEF) by competitive hybridization of a microarray. A total of 270 and 173 genes were two-fold up- and down-regulated with FDR < 0.05 in the hBMSC compared to the hEF, respectively. The overexpressed genes in the hBMSC over the hEF, including transcription factors, were enriched for biological processes such as axial pattern formation, face morphogenesis and skeletal system development, which could be expected from the differentiation potential of MSCs. CD70 and CD339 were identified as additional CD markers that were up-regulated in the hBMSC over the hEF. The differential expression of CD70 and CD339 might be exploited to distinguish hEF and hBMSC. CMKLR1, a chemokine receptor, was up-regulated in the hBMSC compared to the hEF. RARRES2, a CMKLR1 ligand, stimulated specific migration of the hBMSC, but not of the hEF. RARRES2 manifested as ~two-fold less effective than SDF-1α in the directional migration of the hBMSC. The expression of CMKLR1 was decreased upon the osteoblastic differentiation of the hBMSC. However, the RARRES2-loaded 10% HA-silk scaffold did not recruit endogenous cells to the scaffold in vivo. The RARRES2–CMKLR1 axis could be employed in recruiting systemically delivered or endogenous MSCs to a specific target lesion.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts

Cho

Lee

Park

et al. 2022

CIMB

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“…Transdermal illumination and irradiation through blood vessel walls have both been used in situ to produce minimally invasive photopolymerization [5]. The modification of oligo [poly(ethylene glycol) fumarate] (OPF) polymer hydrogel scaffolds with polyethylene glycol (PEG) creates a hydrogel with a macroporous three-dimensional structure that can produce a stable polymer network through chemical crosslinking with controllable mechanical strength and low immunogenicity [6,7]. However, most of the hydrogels based on PEG exhibit poor biocompatibility and are not efficient with regards to promoting cell growth and proliferation [8].…”

Section: Introductionmentioning

confidence: 99%

An Injectable Hydrogel Scaffold Loaded with Dual-Drug/Sustained-Release PLGA Microspheres for the Regulation of Macrophage Polarization in the Treatment of Intervertebral Disc Degeneration

Cheng

Guo

Zhao

et al. 2022

IJMS

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Due to the unique physical characteristics of intervertebral disc degeneration (IVDD) and the pathological microenvironment that it creates, including inflammation and oxidative stress, effective self-repair is impossible. During the process of intervertebral disc degeneration, there is an increase in the infiltration of M1 macrophages and the secretion of proinflammatory cytokines. Here, we designed a novel injectable composite hydrogel scaffold: an oligo [poly (ethylene glycol) fumarate]/sodium methacrylate (OPF/SMA) hydrogel scaffold loaded with dual-drug/sustained-release PLGA microspheres containing IL-4 (IL-4-PLGA) and kartogenin (KGN-PLGA). This scaffold exhibited good mechanical properties and low immunogenicity while also promoting the sustained release of drugs. By virtue of the PLGA microspheres loaded with IL-4 (IL-4-PLGA), the composite hydrogel scaffold induced macrophages to transition from the M1 phenotype into the M2 phenotype during the early induced phase and simultaneously exhibited a continuous anti-inflammatory effect through the PLGA microspheres loaded with kartogenin (KGN-PLGA). Furthermore, we investigated the mechanisms underlying the immunomodulatory and anti-inflammatory effects of the composite hydrogel scaffold. We found that the scaffold promoted cell proliferation and improved cell viability in vitro. While ensuring mechanical strength, this composite hydrogel scaffold regulated the local inflammatory microenvironment and continuously repaired tissue in the nucleus pulposus via the sequential release of drugs in vivo. When degenerative intervertebral discs in a rat model were injected with the scaffold, there was an increase in the proportion of M2 macrophages in the inflammatory environment and higher expression levels of type II collagen and aggrecan; this was accompanied by reduced levels of MMP13 expression, thus exhibiting long-term anti-inflammatory effects. Our research provides a new strategy for promoting intervertebral disc tissue regeneration and a range of other inflammatory diseases.

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“…Stromal derived factor-1α (SDF-1α), also known CXCL12, is a member of the CXC chemokine family that binds specifically to CXCR4 cell membrane receptors [ 10 , 11 ]. Many studies have shown that SDF-1α/CXCR4 axis plays an important role in promoting the recruitment of BMSCs to bone defects through its chemotactic effect [ 12 , 13 , 14 ]. As a hydrogel formed by natural polymer, XG hydrogels enable to simulate the characteristics of natural extracellular matrices and release the bioactive factors as scaffolds [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Controlled and Sequential Delivery of Stromal Derived Factor-1 α (SDF-1α) and Magnesium Ions from Bifunctional Hydrogel for Bone Regeneration

Lin

Shi

et al. 2022

Polymers

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Bone healing is a complex process that requires the participation of cells and bioactive factors. Stromal derived factor-1 α (SDF-1α) and magnesium ions (Mg2+) both are significant bioactive factors for cell recruitment and osteogenesis during bone regeneration. Thus, a bifunctional hydrogel containing a sequential delivery system is fabricated to improve osteogenesis. During sequential delivery of the hydrogel, SDF-1α is predominantly released at the early stage of bone mesenchymal stem cells (BMSCs) recruitment, while Mg2+ are constantly delivered at a later stage to improve osteogenic differentiation of recruited cells. In addition, due to the early release of SDF-1α, the hydrogel showed strong BMSCs recruitment and proliferation activity. Mg2+ can not only induce up-regulation of osteogenic gene expression in vitro, but also promote bone tissue and angiogenesis in vivo. Taken together, the injection of xanthan gum-polydopamine crosslinked hydrogel co-loading SDF-1α and Mg2+ (XPMS hydrogel) provides a novel strategy to repair bone defects.

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SDF-1α/OPF/BP Composites Enhance the Migrating and Osteogenic Abilities of Mesenchymal Stem Cells

Cited by 7 publications

References 31 publications

Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts

Competitive Hybridization of a Microarray Identifies CMKLR1 as an Up-Regulated Gene in Human Bone Marrow-Derived Mesenchymal Stem Cells Compared to Human Embryonic Fibroblasts

An Injectable Hydrogel Scaffold Loaded with Dual-Drug/Sustained-Release PLGA Microspheres for the Regulation of Macrophage Polarization in the Treatment of Intervertebral Disc Degeneration

Controlled and Sequential Delivery of Stromal Derived Factor-1 α (SDF-1α) and Magnesium Ions from Bifunctional Hydrogel for Bone Regeneration

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