Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation

Sensory systems are specialized to recognize environmental changes. Sensory organs are complex structures composed of different cell types, including neurons and sensory receptor cells, and how these organs are generated is an important question in developmental neurobiology.The posterior lateral line (pLL) is a simple sensory system in fish and amphibians that detects changes in water motion. It consists of neurons and sensory receptor hair cells, both of which are derived from the cranial ectoderm preplacodal region. However, it is not clearly understood how neurons and the sensory epithelium develop separately from the same preplacodal progenitors.We found that the numbers of posterior lateral line ganglion (pLLG) neurons, which are marked by neurod expression, increased in embryos with reduced Notch activity, but the forced activation of Notch reduced their number, suggesting that Notch-mediated lateral inhibition regulates the pLLG cell fate in zebrafish. By fate-mapping analysis, we found that cells adjacent to the pLLG neurons in the pre-pLL placodal region gave rise to the anterior part of the pLL primordium (i.e., sensory epithelial progenitor cells), and that the choice of cell fate between pLLG neuron or pLL primordium was regulated by Notch signaling. Since Notch signaling also affects hair cell fate determination at a later stage, our study suggests that Notch signaling has dual, time-dependent roles in specifying multiple cell types during pLL development.

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“…Time-lapse analysis was performed as described previously (Mizoguchi et al, 2008). Time-lapse movies were generated using ImageJ (NIH).…”

Section: Methodsmentioning

confidence: 99%

Neuron and Sensory Epithelial Cell Fate Is Sequentially Determined by Notch Signaling in Zebrafish Lateral Line Development

Mizoguchi

Togawa²,

Kawakami

et al. 2011

J. Neurosci.

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“…In the mutant germ cell cultures, four testes were used per 35-mm culture dish and were cultured for 8 days. In co-cultures of wild-type and mutant germ cells, cells from two wild-type Tg(sox17:EGFP) transgenic zebrafish (Mizoguchi et al, 2008) testes and two mutant testes were mixed. At 1 day postfertilization (1 dpf), GFP signals were observed under a fluorescent microscope.…”

Section: Male Zebrafish Germ Cell Culturesmentioning

confidence: 99%

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Saito

Siegfried

Nüsslein‐Volhard

et al. 2011

Developmental Dynamics

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We describe here the isolation and cytogenetic characterization of three meiotic prophase I mutants, denoted ietsugu (its), iesada (isa), and iemochi (imo), isolated by a novel N-ethyl-N-nitrosourea mutagenesis screen for adult zebrafish gonadogenesis. Histological examination and flow cytometry analysis of testes from these mutants showed that each contained neither spermatids nor sperm. Staining for Sycp3 and cleaved Caspase-3 and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridinetriphosphate nick end-labeling) assay further revealed that its had defects at the onset of meiosis, and that isa and imo spermatocytes failed to progress past the zygotene stage with apoptosis occurring in the testicular somatic cells. Staining for phosphorylated histone H2AX showed that foci formation in leptotene spermatocytes was disrupted in isa and imo. Furthermore, in vitro differentiation experiments revealed the possibility that the defects and sterility associated with mutations were germ line autonomous. Our results thus indicate that each responsible gene is necessary for meiotic progression during spermatogenesis and for male fertility.

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“…One CXC-type ligand, SDF1, plays an important role in gastrulation (Fukui et al 2007;Mizoguchi et al 2008;Nair & Schilling 2008), and SDF interacts with CXCR4 and CXCR7 (Valentin et al 2007;Tiveron & Cremer 2008). We investigated whether CXCR4 and CXCR7 interact with XCXCLC.…”

Section: Receptors For Xcxclcmentioning

confidence: 99%

Chemokine ligand Xenopus CXCLC (XCXCLC) regulates cell movements during early morphogenesis

Goto¹,

Asashima²

2011

Development, Growth &Amp; Differentiation

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We cloned the gene for the CXC-type chemokine ligand, Xenopus CXCLC (XCXCLC), the transcripts of which were detected at the dorsal midline during the gastrula and neurula stages. XCXCLC overexpression resulted in the attraction of nearby mesodermal cells, and the excess of chemoattractant interfered with convergent and extension movements. The direction of the deep neural plate cells around the notoplate was also controlled by XCXCLC. Fluorescence signals for XCXCLC + enhanced green fluorescent protein derivatives accumulated around the notochord region. These results indicate that XCXCLC attracts adjacent cells to the midline region, so as to ensure accurate lateral-medial directional tissue convergence during gastrulation and neurulation.

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Sdf1/Cxcr4 signaling controls the dorsal migration of endodermal cells during zebrafish gastrulation

Cited by 172 publications

References 45 publications

Neuron and Sensory Epithelial Cell Fate Is Sequentially Determined by Notch Signaling in Zebrafish Lateral Line Development

Neuron and Sensory Epithelial Cell Fate Is Sequentially Determined by Notch Signaling in Zebrafish Lateral Line Development

Isolation and cytogenetic characterization of zebrafish meiotic prophase I mutants

Chemokine ligand Xenopus CXCLC (XCXCLC) regulates cell movements during early morphogenesis

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