SDS-PAGE as a Tool for Hydrodynamic Diameter-Dependent Separation of Quantum Dots

Křížková, Soňa; Dostálová, Simona; Michálek, Petr; Nejdl, Lukáš; Komínková, Markéta; Milosavljević, Vedran; Moulick, Amitava; Vaculovičová, Markéta; Kopel, Pavel; Adam, Vojtěch; Kizek, René

doi:10.1007/s10337-015-2893-z

Cited by 11 publications

(11 citation statements)

References 45 publications

(47 reference statements)

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“…CGE is a sensitive, high‐throughput and high‐resolution system and has been used as a routine separation technique for size‐based analysis of nucleic acid , monoclonal antibodies and other types of proteins .…”

Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

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Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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“…Absolute values were calculated using the standard samples (quinine sulfate), with known fluorescence QY value, according to the following Eq. [37]:…”

Section: Determination Of Quantum Yields (Qy)mentioning

confidence: 99%

Real-time monitoring of the UV-induced formation of quantum dots on a milliliter, microliter, and nanoliter scale

et al. 2017

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The authors report on a systematic study on the low-cost, low-temperature, and fast synthesis of water soluble quantum dots (QDs) stabilized by mercaptosuccinic acid by UV irradiation. The effects of UV irradiation (at 254 nm and 250 nm) and temperature on the precursors (Cd:Se, Cd:Te, Cd, Zn:S, Zn:Se and Zn) are described. Best results are achieved with a mixture of precursors containing cadmium, selenium and MSA where a 10-min irradiation with 254-nm light gives CdSe QDs with a quantum yield of 13.5%. The authors also describe the preparation and monitoring of the formation of QDs in sub-mg, sub-μg and sub-ng quantities, the smallest concentration being 258 pg in volume of 4 nL. The growth of the QDs can be monitored in real time by absorption, fluorescence and dynamic light scattering. The solutions of the particles also are characterized by fluorescence correlation spectroscopy and detected by LEDinduced fluorescence. The preparation of such QDs by UV radiation is simple, easily controllable, and inexpensive. Conceivably, it can be integrated with lab-on-chip, micro total analysis systems or other instrumentation.

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“…Krizkova et al reported that they qualitatively measured NP sizes using agarose gel electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). 28 They used SDS-PAGE to separate different sized NPs using similar processes such as traditional NP purification with gel electrophoresis tools. 28 In this study, we have developed a new protocol using SDS-PAGE combined with fluorescence imaging for the detection and quantification of Zinkicide dissolution in aqueous solutions.…”

Section: Introductionmentioning

confidence: 99%

“…28 They used SDS-PAGE to separate different sized NPs using similar processes such as traditional NP purification with gel electrophoresis tools. 28 In this study, we have developed a new protocol using SDS-PAGE combined with fluorescence imaging for the detection and quantification of Zinkicide dissolution in aqueous solutions. The protocol was developed on the premise that Zinkicide particle sizes are comparable to protein dimensions and that they exhibit fluorescent properties when exposed to UV light, which is compatible with conventional gel imaging stations.…”

Section: Introductionmentioning

confidence: 99%

SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions

Untracht¹,

Özcan²,

Santra³

et al. 2020

ACS Omega

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Zinkicide is a systemic bactericidal formulation containing proteinsize fluorescent zinc oxide-based nanoparticles (nano-ZnO). Previous studies have shown that Zinkicide is effective in controlling citrus diseases. Its field performance as an antimicrobial agent has been linked to the bioavailability of zinc ions (Zn 2+ ) at the target site. It is therefore important to monitor Zn 2+ release from Zinkicide so that application rates and frequency can be estimated. In this study, we present a simplistic approach designed to monitor Zinkicide nanoparticle dissolution rates in water and acidic buffer solutions using traditional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The evolution of nano-ZnO in the polyacrylamide gel scaffolds was studied by exciting the sample with UV light and detecting the fluorescence of nano-ZnO. Fluorescence intensities measured with this assay allowed for quantitative analysis of molecular weight changes of nano-ZnO in citrate buffer, a surrogate of citrus juice. Our results demonstrated that citrate buffer induced the greatest degradation of Zinkicide. Fluorescence intensity fluctuations were observed over time, indicating interactions of citrate with the surface of nano-ZnO. These findings provide a new approach to quantify the dissolution of nanoparticles in simulated environments, even when other analytical methods lack sensitivity because of the small size of the system (≈4 nm).

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SDS-PAGE as a Tool for Hydrodynamic Diameter-Dependent Separation of Quantum Dots

Abstract: range 0-4 mM was used to improve visualization of QDs with diameters in the range from 7 ± 2 to 4 ± 2 nm.

Cited by 11 publications

References 45 publications

Recent advances in microscale separation

Recent advances in microscale separation

Real-time monitoring of the UV-induced formation of quantum dots on a milliliter, microliter, and nanoliter scale

SDS-PAGE for Monitoring the Dissolution of Zinc Oxide Bactericidal Nanoparticles (Zinkicide) in Aqueous Solutions

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