SDS polyacrylamide gel electrophoresis and serological analysis of pili from Escherichia coli of different pathogenic origin

Fimbrial adhesins enable bacteria to attach to eucaryotic cells. The genetic determinants for S fimbrial adhesins (sfa) and for FlC ("pseudotype I") fimbriae (foc) were compared. Sfa and FlC represent functionally distinct adhesins in their receptor specificities. Nevertheless, a high degree of homology between both determinants was found on the basis of DNA-DNA hybridizations. Characteristic differences in the restriction maps of the corresponding gene clusters, however, were visible in regions coding for the fimbrial subunits and for the S-specific adhesin. While a plasmid carrying the genetic determinant for FlC fimbriae was able to complement transposon-induced sfa mutants, a plasmid carrying the genetic determinant for a third adhesin type, termed P fimbriae, was unable to do so. Proximal sfa-specific sequences carrying the S fimbrial structural gene were fused to sequences representing the distal part of thefoc gene cluster to form a hybrid cluster, and the foc proximal region coding for the structural protein was ligated to sfa distal sequences to form a second hybrid. Both hybrid clones produced intact fimbriae. Anti-FlC monoclonal antibodies (MAbs) only recognized clones which produced FlC fimbriae, and an anti-S adhesin MAb marked clones which expressed the S adhesin. However, one of four other anti-S fimbriae-speciflic MAbs reacted with both fimbrial structures, S and F1C, indicating a common epitope on both antigens. The results presented here support the view that sfa and foc determinants code for fimbriae that are similar in several aspects, while the P fimbriae are members of a more distantly related group.Fimbriae or pili are bacterial cell wall appendages which consist of about 1,000 identical protein subunits. A single fimbria is 7 nm in diameter and up to 1 ,um in length (9, 17). Very often fimbriae are associated with adhesins which enable the bacteria to attach to eucaryotic cells, including erythrocytes (RBC) (28, 31). Adherence of bacteria to eucaryotic cells is a prerequisite for many infectious diseases (27,30,41).Extraintestinal Escherichia coli isolates cause infections of the urinary tract and the blood (sepsis) and are also the causative agents of newborn meningitis (32). Such E. coli isolates may carry different types of fimbrial adhesins. P fimbriae recognize an ot-D-galactosyl-(1-4)-f3-D-galactose receptor. They are strongly associated with uropathogenic E. coli strains and can be further subdivided into nine serologically distinct groups (F71 through F13) (16,31,41). The P fimbrial gene clusters of different serogroups are functionally related. This was shown by the construction of hybrid clones consisting of DNA sequences derived from different P determinants (cis-complementation) and also by the fact that P-specific gene products were able to produce a wild-type phenotype of P insertion mutants (trans-complementation) (24,36,43,45). Another group of E. coli gene clusters, coding for type I fimbrial adhesins which interact with ct-D-mannose-containing receptors, are also complem...

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“…The filters Preparation of fimbriae. Fimbriae were isolated from bacteria grown on plates as described previously (15).…”

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confidence: 99%

“…The 018: K5 strain 2980 produces P fimbriae (serogroup F8) and FlC fimbriae; strain A21 (07:K1) exhibits P fimbriae. The strains were described previously (15,33,46). For transformation and as controls in Southern hybridizations, the E. coli K-12 strains HB101 and 5K were used.…”

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confidence: 99%

Gene clusters for S fimbrial adhesin (sfa) and F1C fimbriae (foc) of Escherichia coli: comparative aspects of structure and function

et al. 1988

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“…of the subunit proteins (Jann et al, 1981). Molecular cloning of the genes involved in the expression of a number of MRHA fimbriae has been reported (Hull et al, 1981;Clegg, 1982;Rhen et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence of the gene encoding the F72 fimbrial subunit of a uropathogenic Escherichia coli strain

Die

Bergmans

1984

Gene

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SUMMARYThe cloned DNA fragment encoding the F7, timbrial subunit from the uropathogenic Exherichia coli strain AD1 10 has been identified. The nucleotide sequence of the structural gene and of 196 bp of the noncoding region preceding the gene was determined. The structural gene codes for a polypeptide of 188 amino acid residues, including a 2 l-residue N-terminal signal sequence. The nucleotide sequence and the deduced amino acid sequence of the F7, gene were compared with the reported sequences of the pupA gene (Baga et al., 1984). Both genes code for subunits of fimbriae that are involved in mannose-resistant hemagglutination (MRHA) of human erythrocytes. The available data show that there is absolute homology between the noncoding regions preceding both genes over 129 bp. The two proteins are homologous at the N terminus and C terminus; there is less, but significant, homology in the region between the N and C termini.

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“…Pyelonephritogenic E. coli may express various fimbriaecontaining adhesins that have been found to recognize different renal receptors and structures (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). P fimbriae associated with acute pyelonephritis provide an attachment mechanism to ␣ D-Gal-(1,4)-␤ -D-Gal-containing receptors residing on the luminal apical surface of the renal tubules (7,10,12,13).…”

Section: Introductionmentioning

confidence: 99%

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

et al. 1997

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SDS polyacrylamide gel electrophoresis and serological analysis of pili from Escherichia coli of different pathogenic origin

Cited by 23 publications

References 8 publications

Gene clusters for S fimbrial adhesin (sfa) and F1C fimbriae (foc) of Escherichia coli: comparative aspects of structure and function

Gene clusters for S fimbrial adhesin (sfa) and F1C fimbriae (foc) of Escherichia coli: comparative aspects of structure and function

Nucleotide sequence of the gene encoding the F72 fimbrial subunit of a uropathogenic Escherichia coli strain

Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae: mutation in the dra region prevented tubulointerstitial nephritis.

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