Se metallomics during lactic fermentation of Se-enriched yogurt

Palomo, María; Gutiérrez, Ángel; Pérez-Conde, M.C.; Cámara, Carmen; Madrid, Yolanda

doi:10.1016/j.foodchem.2014.05.007

Cited by 52 publications

(27 citation statements)

References 25 publications

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“…Studies have also shown the benefits of the uptake of adequate amounts of Se due to its anticarcinogenic, antimutagenic, antioxidative, antipathogenic, and antiinflammatory properties (Shen et al, 2010;Fairweather-Tait et al, 2011;Ren et al, 2011;Rayman, 2012;Kieliszek and Błazejak, 2013;Yazdi et al, 2013;Alfthan et al, 2015). Discoveries regarding the importance of Se for human health have aroused interest in research involving the bioaccumulation of this trace element in different LAB species with probiotic potential and their use in creating enriched and functional foods (Fisinin et al, 2009;Alzate et al, 2010;Pieniz et al, 2011;Palomo et al, 2014;Vodnar and Socaciu, 2014;Deng et al, 2015;Pusztahelyi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer

Dullius

et al. 2018

Journal of Dairy Science

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Selenium is an essential micronutrient for living beings, as it helps to maintain the normal physiological functions of the organism. The numerous discoveries involving the importance of this element to the health of human beings have fostered interest in research to develop enriched and functional foods. The present study evaluated the potential for bacterial strains of Enterococcus faecalis (CH121 and CH124), Lactobacillus parabuchneri (ML4), Lactobacillus paracasei (ML13, ML33, CH135, and CH139), and Lactobacillus plantarum (CH131) to bioaccumulate Se in their biomass by adding different concentrations of sodium selenite (30 to 200 mg/L) to the culture medium. Quantification of Se with UV and visible molecular absorption spectroscopy showed that the investigated bacteria were able to bioaccumulate this micromineral into their biomass. Two of the L. paracasei strains (ML13 and CH135) bioaccumulated the highest Se concentrations (38.1 ± 1.7 mg/g and 40.7 ± 1.1 mg/g, respectively) after culture in the presence of 150 mg/L of Se. This bioaccumulation potential has applications in the development of dairy products and may be an alternative Se source in the diets of humans and other animals.

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Section: Introductionmentioning

confidence: 99%

Assessment of selenium bioaccumulation in lactic acid bacteria

Mörschbächer

Dullius

et al. 2018

Journal of Dairy Science

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“…Moreover, selenocysteine residue decomposition during sample preparation may occur. To overcome these problems, SeCys residues were alkylated with iodoacetamide prior to enzymatic hydrolysis [ 18 ] Extracts were analyzed by HPLC-ICP-MS. The anion-exchange chromatogram in Figure 6 shows a Se-containing peak at 3 min that was identified as the carbomethylated derivative of SeCys.…”

Section: Resultsmentioning

confidence: 99%

“…Very little data on the effect of SeNPs on lactic acid bacteria has been published. The accumulation and biotransformation of selenium by lactic bacteria has appeared in several papers [ 16 , 17 , 18 , 19 , 20 ]. These studies evidence that selenium, once accumulated by Lactobacillus species is biotransformed into selenoamino acids such as selenomethionine (SeMet) and selenocysteine (SeCys) However, in all above-mentioned studies, selenium was supplemented as inorganic selenium.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Behavior and Metabolic Transformations of SeNPs in Exposed Lactic Acid Bacteria. Effect of Nanoparticles Coating Agent

Palomo-Siguero

Madrid

2017

IJMS

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The behavior and transformation of selenium nanoparticles (SeNPs) in living systems such as microorganisms is largely unknown. To address this knowledge gap, we examined the effect of three types of SeNP suspensions toward Lactobacillus delbrueckii subsp. bulgaricus LB-12 using a variety of techniques. SeNPs were synthesized using three types of coating agents (chitosan (CS-SeNPs), hydroxyethyl cellulose (HEC-SeNPs) and a non-ionic surfactant, surfynol (ethoxylated-SeNPs)). Morphologies of SeNPs were all spherical. Transmission electron microscopy (TEM) was used to locate SeNPs in the bacteria. High performance liquid chromatography (HPLC) on line coupled to inductively coupled plasma mass spectrometry (ICP-MS) was applied to evaluate SeNP transformation by bacteria. Finally, flow cytometry employing the live/dead test and optical density measurements at 600 nm (OD600) were used for evaluating the percentages of bacteria viability when supplementing with SeNPs. Negligible damage was detected by flow cytometry when bacteria were exposed to HEC-SeNPs or CS-SeNPs at a level of 10 μg Se mL−1. In contrast, ethoxylated-SeNPs were found to be the most harmful nanoparticles toward bacteria. CS-SeNPs passed through the membrane without causing damage. Once inside, SeNPs were metabolically transformed to organic selenium compounds. Results evidenced the importance of capping agents when establishing the true behavior of NPs.

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“…Also, it may be due to other bioactive compounds like se-exopolysaccharide and an organic acid that possesses potent antioxidant activity and can be produced by some bacterial strains, such as Enterobacter cloacae Z0206 when grown in a Se-enriched medium [34]. Another study of the production of Se-enriched yogurt by lactic acid bacteria revealed that two proteins involved in antioxidant functions (thioredoxin and glutaredoxin) were detected in the selenoprotein extract [35]. Therefore, in this study, we conclude that isolated organic Se-enriched strains showed in-vitro antioxidant properties, however, the particular mechanism needs to be elucidated in further research.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Identification of Organic Selenium-enriched Bacteria Isolated from Rumen Fluid and Hot Spring Water

Dalia¹,

Loh²,

Sazili³

et al. 2017

Microbiology and Biotechnology Letters

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In the present study, the isolation of selenium (Se)-enriched bacteria from rumen fluid and hot spring water was carried out. Rumen fluid samples were taken from cannulated goats fed a basal diet and the water samples were collected from Selayang hot spring, Selangor-Malaysia. A total number of 140 Se-tolerant isolates were obtained aerobically using an Se-enriched medium and spread plate technique. All the isolates were initially screened for the ability to transform the Se-containing medium to a red-orange culture using a spectrophotometer. Twenty isolates of dark red-orange medium were selected for a screening of the highest Se-containing protein accumulating strains using the dialysis technique and icp.ms to measure the Se content. Four isolates, identified as Enterobacter cloacae (ADS1, ADS7, and ADS11), and Klebsiella pneumoniae (ADS2) from rumen fluid origin, as well as, one isolate from hot spring water (Stenotrophomonas maltophilia (ADS18)), were associated with the highest biomass organic Se-containing protein when grown in a medium enriched with 10 µg/ml sodium selenite. In addition, around 50 µg/100 µg of the absorbed inorganic Se was accumulated as an organic form. Organic Se-containing protein in all the selected strains showed antioxidant properties in the range of 0.306 to 0.353 Trolox equivalent antioxidant capacity (TEAC) mg/ml. Therefore, these strains may offer a potential source of organic Se due to their Se-tolerant nature and higher biomass organic to inorganic Se ratio.

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Se metallomics during lactic fermentation of Se-enriched yogurt

Cited by 52 publications

References 25 publications

Assessment of selenium bioaccumulation in lactic acid bacteria

Assessment of selenium bioaccumulation in lactic acid bacteria

Exploring the Behavior and Metabolic Transformations of SeNPs in Exposed Lactic Acid Bacteria. Effect of Nanoparticles Coating Agent

Characterization and Identification of Organic Selenium-enriched Bacteria Isolated from Rumen Fluid and Hot Spring Water

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