Sea cucumber (Stichopus hermanii) based hydrogel to treat burn wounds in rats

Abstract

Malaysian sea cucumber was incorporated into hydrogel formulation by using electron beam irradiation technique and was introduced as novel cross-linked Gamat Hydrogel dressing. This study investigated whether Gamat Hydrogel enhanced repair of deep partial skin thickness burn wound in rats and its possible mechanism. Wounds were treated with either Gamat Hydrogel, control hydrogel, OpSite V R film dressing or left untreated. Skin samples were taken at 7, 14, 21, and 28 days post burn for histological and molecular evaluations. Gamat Hydrogel markedly enhanced wound contraction and improved histological reorganization of the regenerating tissue. Furthermore, the dressing modulated the inflammatory responses, stimulated the activation and proliferation of fibroblasts, and enhanced rapid production of collagen fiber network with a consequently shorter healing time. The level of proinflammatory cytokines; IL-1a, IL-1b, and IL-6, were significantly reduced in Gamat Hydrogel treated wounds compared with other groups as assessed by reverse transcription-polymerase chain reaction (RT-PCR). In summary, our results showed that Gamat Hydrogel promoted burn wound repair via a complex mechanism involving stimulation of tissue regeneration and regulation of pro-inflammatory cytokines. The resultant wound healing effects were attributed to the synergistic effect of the hydrogel matrix and incorporated sea cucumber.

Polyurethane for biomedical applications: A review of recent developments

“…Some medicinal agents, such as pharmacological active agents, antibacterial agents, topical anesthetics, bacteriostatic agents and antifungal agents, are often incorporated into the dressings, dependent on the specifi c applications. Many wound dressings made of different materials have been studied and reported, such as hydrocolloid (Jeong et al, 2011), hydrogel (Zohdi et al, 2011), medicated dressings (Yu et al, 2006) and liquid bandages (Martin-Garcia et al, 2005). Klode et al (2011) investigated all kinds of wound dressings, totaling 56 used nowadays, and presented a useful data source that can be used to develop a specifi c wound dressing.…”

FGF-2, MMP-8 and Integrin α2β1 Expression in Periodontal ligament Remodelling Tension Area with Nanopowder <i>Stichopus hermanii</i> Application to Prevent Orthodontic Relapsing

“…Stichopus hermanii can act as anticandidal [8], [9] Stichopus hermanii treated wounds and stimulation tissue regeneration. [10]. The other study show that studies have shown that the extract of Stichopus species also affects viability or proliferation of human fibroblasts and osteoclast cells in a negative manner [10].…”

Polymeric hydrogels for burn wound care: Advanced skin wound dressings and regenerative templates

“…infection control, dressing flexibility). [35–52] In situ forming gels[38,53–56] and radiation-crosslinked gels[36,44,45,57] are also appealing for the development of novel wound dressings.

Figure 3:Increasing number of research articles concerning the development of hydrogel-based wound dressings, published in the last ten years (Source: PubMed. Keywords: hydrogel wound dressing; burn.

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