Search for diarrheagenic Escherichia coli in raw kibbe samples reveals the presence of Shiga toxin-producing strains

Peresi, Jacqueline Tanury Macruz; Almeida, Ivete Aparecida Zago Castanheira de; Vaz, Tânia; Hernandes, Rodrigo T.; Teixeira, Inara Siqueira de Carvalho; Silva, Sonia Izaura de Lima e; Graciano, Rejane Alexandre Silva; Pinheiro, Sandra Regina Schicariol; Santos, Luis Fernando dos

doi:10.1016/j.foodcont.2015.11.018

Cited by 17 publications

(8 citation statements)

References 56 publications

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“…Although data on the detection of EHEC/STEC in foods in Brazil are still scarce, the isolation and identification of O157:H7 serotype from a ground beef sample was described for the first time, 189 while O125:H19 and O149:H8 STEC serotypes were found in refrigerated raw kibbe collected from retail establishments 190 . On the other hand, EHEC/STEC has not been detected in pasteurized cow's milk samples collected in dairies in northwestern Paraná State 191 or in raw milk, pasteurized milk, Minas Frescal cheese and ground beef samples collected in Minas Gerais 192 .…”

Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

436

353

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Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host. According to the group of virulence determinants acquired, specific combinations were formed determining the currently known E. coli pathotypes, which are collectively known as diarrheagenic E. coli. In this review, we have gathered information on current definitions, serotypes, lineages, virulence mechanisms, epidemiology, and diagnosis of the major diarrheagenic E. coli pathotypes.

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Section: Enterohemorrhagic (Shiga Toxin-producing) E Coli (Ehec/stec)mentioning

confidence: 99%

Diarrheagenic Escherichia coli

Gomes

Elias

Scaletsky

et al. 2016

Brazilian Journal of Microbiology

436

353

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“…Twenty-nine of the 43 strains had been previously investigated regarding their serotypes, stx gene types and subtypes, the presence of the eae gene and the ability to produce Shiga toxins in Vero cells cultivated in vitro [29]. Presently, the remaining 15 strains had the same characteristics determined by standard procedures [30][31][32]. Moreover, since some of the strains previously serotyped by tube agglutination tests were phenotypically non-motile, all the 43 strains were now further analyzed for flagellar (H) antigens by using a multiplex PCR strategy with specific primers directed to each of the fliC gene alleles [33].…”

Section: Bacterial Strainsmentioning

confidence: 99%

Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil

et al. 2020

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Since no recent data characterizing Shiga toxin-producing E. coli (STEC) from human infections in Brazil are available, the present study aimed to investigate serotypes, stx genotypes, and accessory virulence genes, and also to perform pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) of 43 STEC strains recovered from 2007 to 2017. Twenty-one distinct serotypes were found, with serotype O111:H8 being the most common. However, serotypes less frequently reported in human diseases were also found and included a hybrid STEC/ETEC O100:H25 clone. The majority of the strains carried stx1a as the sole stx genotype and were positive for the eae gene. Regarding the occurrence of 28 additional virulence genes associated with plasmids and pathogenicity islands, a diversity of profiles was found especially among the eae-harboring strains, which had combinations of markers composed of up to 12 distinct genes. Although PFGE analysis demonstrated genetic diversity between serotypes such as O157:H7, O111:H8, O26:H11, O118:H16, and O123:H2, high genetic relatedness was found for strains of serotypes O24:H4 and O145:H34. MLST allowed the identification of 17 distinct sequence types (STs) with ST 16 and 21 being the most common ones. Thirty-five percent of the strains studied were not typeable by the currently used MLST approach, suggesting new STs. Although STEC O111:H8 remains the leading serotype in Brazil, a diversity of other serotypes, some carrying virulence genes and belonging to STs incriminated as causing severe disease, were found in this study. Further studies are needed to determine whether they have any epidemiological relevance.

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“…Among the most important genes, stx has been associated with E. coli strains producing Shiga toxin (STEC). STEC are one of the most important strains, mainly due to their ability to cause an array of diseases (Peresi et al 2016;Li et al 2016). The genes st (enterotoxin heat-stable) and lt (enterotoxin heat-labile) have been associated with enterotoxigenic E. coli (ETEC), the most common cause of childhood diarrhea (Canizalez-Roman et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance in diarrheagenic Escherichia coli from ready-to-eat foods

et al. 2017

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Certain subgroups of have congenital or acquired virulence properties that allow them to cause a wide spectrum of disease. The aim of this study was to investigate the occurrence of diarrheagenic strains in ready-to-eat (RTE) foods produced in institutional, commercial and hotel restaurants in Salvador, Brazil. The presence of virulent isolates and antimicrobial resistance were evaluated. Four hundred forty-six samples were collected and grouped into cereals and vegetables, meat-based preparations, cooked salads, raw salads, garnishes, soups and sauces, desserts and juices. were detected using the most probable number method, the presence of virulence factors in isolates was determined by polymerase chain reaction (PCR) assays, and antibiotic resistance was analyzed using the disc diffusion method. In total, 15 isolates (3.1%) of were recovered; raw salads had the highest detection rate, 1.4%, followed by cooked salads, 0.8%; meat-based preparations, 0.4%; and cereals and vegetables, 0.4%. PCR assays showed that none of the isolates had the virulence genes , or . The isolates showed resistance to nine antibiotics of the 15 tested, and the highest levels of resistance were found for sulfamethoxazole/trimethoprim, tetracycline, ampicillin, and chloramphenicol (13.3% of isolates for each antibiotic). One isolate from cooked salad had plasmid-mediated multidrug resistance to tetracycline, trimethoprim/sulfamethoxazole, ampicillin and chloramphenicol. These results suggest that RTE foods, especially raw salads, can be reservoirs of and facilitate the spread of antibiotic resistance genes to the gastrointestinal microbiota of humans.

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Search for diarrheagenic Escherichia coli in raw kibbe samples reveals the presence of Shiga toxin-producing strains

Cited by 17 publications

References 56 publications

Diarrheagenic Escherichia coli

Diarrheagenic Escherichia coli

Virulence Profiling and Molecular Typing of Shiga Toxin-Producing E. coli (STEC) from Human Sources in Brazil

Antimicrobial resistance in diarrheagenic Escherichia coli from ready-to-eat foods

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