Search of effective IRAP markers for sakura genotyping

Stepanov, I. V.; Balapanov, Ilnur Malikovich; Drygina, Anna Igorevna

doi:10.1051/bioconf/20202503006

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…In total, 2 ISSR markers and 2 IRAP markers were used. The markers were previously tested on sakura [13,14]. PCR was carried out according to the following program: 3 minutes of preliminary denaturation at the temperature of 95 °C; subsequent 35 cycles: denaturation 35 seconds at 95 °C, primer annealing 1 minute at 50 °C for ISSR and 55 °C for IRAP, elongation 1.5 minutes at 72 °C, and a final synthesis cycle at 72 °C for 5 minutes.…”

Section: Methodsmentioning

confidence: 99%

Identification of Sakura interspecific hybrids in Prunus collections

2021

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The use of IRAP and ISSR markers for the genetic analysis of Cerasus and Padus samples from the NCFRCHVW collection made it possible to establish the collection genetic structure and identify interspecific hybrids of cherry trees. Clustering of genotyped samples revealed 4 main clusters: 1) Bird cherry; 2) Cherries; 3) Interspecific hybrids of sakura; 4) Sakura. Most of the hybrid forms of sakura and cherries have formed a separate group, which is different from both sour and sweet cherry varieties, and from the classic sakura varieties. Also, some samples were identified that were assigned to groups that were not typical for them. These samples include the genotype of the Sibirskaya krasavitsa bird cherry, AI72 rootstock, Podbelskaya cherry, Polskaya sakura and ornamental cherry Rexii. In general, ISSR and IRAP markers have demonstrated their effectiveness as tools for genetic analysis of Prunus collections and identification of genotypes arising in the course of interspecific hybridization.

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Section: Methodsmentioning

confidence: 99%

Identification of Sakura interspecific hybrids in Prunus collections

2021

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Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

Bidyananda,

Jamir,

Nowakowska

et al. 2024

IJPB

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The plant adaptation response to a changing environment depends on the genetic diversity level it possesses. Genetic diversity and a thorough understanding of population indices are pivotal for decoding plant adaptation to dynamic environmental stressors. The development of polymerase chain reaction (PCR)-based molecular markers enables comprehensive population analyses and the precise detection of individuals and groups with unique genetic variations. Various molecular markers have been employed to assess genetic diversity, examine population structure, and delineate cluster patterns within and among populations. DNA markers revolutionize plant diversity studies by allowing detailed analyses of genetic variations, including economically significant trait-influencing genes. Despite their simplicity, they offer high reproducibility, ensuring accurate estimations of plant variation. Integrating multiple marker systems with advanced high-throughput sequencing techniques is poised to enhance the understanding and management of depleting plant genetic resources by providing a comprehensive picture of diversity at the genome-wide level. This review explores diverse molecular markers, elucidating their advantages and limitations, and highlights their impact on evaluating the genetic diversity and population structure of plants.

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Search of effective IRAP markers for sakura genotyping

Cited by 2 publications

References 13 publications

Identification of Sakura interspecific hybrids in Prunus collections

Identification of Sakura interspecific hybrids in Prunus collections

Plant Genetic Diversity Studies: Insights from DNA Marker Analyses

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