Searching for antimicrobial photosensitizers among a panel of BODIPYs

Orlandi, Viviana Teresa; Martegani, Eleonora; Bolognese, Fabrizio; Caruso, Enrico

doi:10.1007/s43630-022-00212-4

Cited by 12 publications

(10 citation statements)

References 58 publications

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“…Our results displayed higher biofilm inhibition activity than their findings. Orlandi et al 66 examined the antibiofilm activity of 3 cationic and 12 non‐ionic BODIPY compounds against S. aureus , C. albicans and P. aeruginosa . It was found that some of BODIPY compounds showed antibiofilm activity without light irridiation and also when light irridation exposed they indicated excelsior antibiofilm activity than dark application.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and characterization of novel BODIPYs and their antioxidant, antimicrobial, photodynamic antimicrobial, antibiofilm and DNA interaction activities

Karanlık

Özdemir

et al. 2023

Photochem & Photobiology

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In the current study, we synthesized and characterized new BODIPY derivatives (1–4) having pyridine or thienyl‐pyridine substituents at meso‐ position and 4‐dibenzothienyl or benzo[b]thien‐2‐yl moieties at 2‐,6‐ positions. We investigated fluorescence properties and the ability to form singlet oxygen. In addition, various biological activities of BODIPYs such as DPPH scavenging, DNA binding/cleavage ability, cell viability inhibition, antimicrobial activity, antimicrobial photodynamic therapy (aPDT) and biofilm inhibition properties were performed. BODIPY derivatives BDPY‐3 (3) and BDPY‐4 (4) have high fluorescence quantum yields as 0.50 and 0.61 and 1O2 quantum yields were calculated as 0.83 for BDPY‐1 (1), 0.12 for BDPY‐2 (2), 0.11 for BDPY‐3 and 0.23 for BDPY‐4. BODIPY derivatives BDPY‐2, BDPY‐3 and BDPY‐4 displayed 92.54 ± 5.41%, 94.20 ± 5.50%, and 95.03 ± 5.54% antioxidant ability, respectively. BODIPY compounds showed excellent DNA chemical nuclease activity. BDPY‐2, BDPY‐3 and BDPY‐4 also exhibited 100% APDT activity against E. coli at all tested concentrations. In addition to these, they demonstrated a highly effective biofilm inhibition activity against Staphyloccous aureus and Pseudomans aeruginosa. BDPY‐4 showed the most effective antioxidant and DNA cleavage activity, while BDPY‐3 exhibited the most effective antimicrobial and antibiofilm activity.

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Section: Resultsmentioning

confidence: 99%

Synthesis and characterization of novel BODIPYs and their antioxidant, antimicrobial, photodynamic antimicrobial, antibiofilm and DNA interaction activities

Karanlık

Özdemir

et al. 2023

Photochem & Photobiology

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“…In some cases, however, the importance of PS's binding to bacterial cells has not been confirmed. Among a series of neutral and cationic BODIPYs studied, even though the cationic ones showed a higher binding, unexpectedly, a neutral BODIPY molecule (B9) showed the strongest PDI effect against P. aeruginosa PAO1 24-h-old biofilm [126]. With 40 µM of B9, and after green light activation (520 nm; fluence rate 2.4 mW/cm 2 ; light dose 30 J/cm 2 ), a 5-log reduction of adherent and planktonic cells was reported [126].…”

Section: Pseudomonas Aeruginosamentioning

confidence: 98%

Photodynamic Inactivation of Opportunistic Premise Plumbing Pathogens and Their Biofilms

Mušković,

Gobin,

Malatesti

2023

Processes

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Opportunistic premise plumbing pathogens (OPPPs) form a group of microorganisms that normally live in water supply systems and have adapted especially well to the conditions in premise plumbing systems, and as such pose a threat to human health. Since the beginning of the 21st century, this threat has been escalating, and it is becoming increasingly evident that current water disinfection methods fall short in effectively controlling these pathogens. In researching new approaches to this emergency, phototherapy looks promising, especially one that combines photosensitizers, light, and oxygen, which is known as photodynamic inactivation (PDI). This review describes the main characteristics of the recognized (Pseudomonas aeruginosa, Legionella pneumophila, and Mycobacterium avium) and most important emerging OPPPs, and it offers a brief overview of current disinfection methods and their limitations in the fight against OPPPs. The principle and outcomes of PDI with endogenous and, in particular, exogenous photosensitizers are then explained and described through representative examples of PDI on recognized and emerging OPPPs and their biofilms. Finally, the prospects and future directions of PDI research in water disinfection and control of OPPPs are discussed.

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“…BODIPY (4,4-difluoro-boradiazaindacene) dyes, first reported in 1968, are widely used as fluorescent probes to label biomolecules [20,21]. Recently, increasing attention has been focused on the photodynamic properties of BODIPY due to its large molar absorption coefficients, high fluorescence quantum yields, and tunable modifications [22][23][24]. Our previous studies reported a derivative of BODIPYs, named BDP-4L, as a promising Type II photosensitizer [25,26].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

Wang

Gu²,

Wan

et al. 2023

Photochem Photobiol Sci

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Photodynamic inactivation (PDI) has received increasing attention as a promising approach to combat Candida albicans infections. This study aimed to evaluate the synergistic effect of a new BODIPY (4,4-difluoro-boradiazaindacene) derivative and hydrogen peroxide on C. albicans. BDP-4L in combination with H 2 O 2 demonstrated enhanced photokilling efficacy. In suspended cultures of C. albicans, the maximum decrease was 6.20 log and 2.56 log for PDI using BDP-4L (2.5 μM) with or without H 2 O 2 , respectively. For mature C. albicans biofilms, 20 μM BDP-4L plus H 2 O 2 eradicated C. albicans, causing an over 6.7 log count reduction in biofilm-associated cells, while only a reduction of ~ 1 log count was observed when H 2 O 2 was omitted. Scanning electron microscopy analysis and LIVE/DEAD assays suggested that PDI using BDP-4L plus H 2 O 2 induced more damage to the cell membrane. Correspondingly, amplification of nucleic acids release was observed in biofilms treated with the combined PDI. Additionally, we also discovered that the addition of hydrogen peroxide potentiated the generation of 1 O 2 in PDI using the singlet oxygen sensor green probe. Collectively, BDP-4L combined with H 2 O 2 presents a promising approach in the treatment of C. albicans infections.

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Searching for antimicrobial photosensitizers among a panel of BODIPYs

Cited by 12 publications

References 58 publications

Synthesis and characterization of novel BODIPYs and their antioxidant, antimicrobial, photodynamic antimicrobial, antibiofilm and DNA interaction activities

Synthesis and characterization of novel BODIPYs and their antioxidant, antimicrobial, photodynamic antimicrobial, antibiofilm and DNA interaction activities

Photodynamic Inactivation of Opportunistic Premise Plumbing Pathogens and Their Biofilms

Hydrogen peroxide enhanced photoinactivation of Candida albicans by a novel boron-dipyrromethene (BODIPY) derivative

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