As an important parameter in studies on vision and ophthalmology, pupil size directly controls the amount of the light entering the human eye. Pupilometers or eye tracking systems are used to measure the pupil size in some studies while a pupil size estimation model can be applied when actual measurement is not available, such as in cases of optical design, photobiological safety studies, etc. There are several existing pupil size estimation models, most of which reveal the relationship between pupil size and spatial distribution of luminance. However, the radial asymmetry of spatial luminance weighting function in horizontal and vertical directions and the effect of light chromaticity on the human eye were investigated. In this paper, experiments were conducted to reveal the asymmetry of the spatial weighting function in the horizontal and vertical directions and the effect of chromaticity on the pupil size. A unified pupil size estimation model is proposed based on spatially weighted corneal flux density and chromaticity coordinates.