Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model

Holzbach, T.; Neshkova, Iva; Vlaskou, Dialekti; Konerding, Moritz A.; Gänsbacher, Bernd; Biemer, E.; Giunta, Riccardo E.

doi:10.1016/j.bjps.2008.05.036

Cited by 43 publications

(34 citation statements)

References 38 publications

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“…E-mail: timo@ spanholtz.net. by tissue hypoxia [6] leads to the activated transcription of numerous genes, including VEGF [6], but appears to be only one facet of a complex system of angiogenesis and arteriogenesis. Obviously, high levels of VEGF 165 or basic fibroblast growth factor (bFGF) alone do not lead to functional arteriogenesis [7,8].…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Spanholtz

Theodorou

Holzbach

et al. 2011

Journal of Surgical Research

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Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Spanholtz

Theodorou

Holzbach

et al. 2011

Journal of Surgical Research

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“…The optimal time delay has been reported as 2 or 3 weeks, depending on species . A delay as short as 5 days in a rat model has been recently suggested, but is not yet supported by any clinical evidence …”

Section: Discussionmentioning

confidence: 99%

Complications of the angularis oris axial pattern buccal flap for reconstruction of palatine defects in two dogs

Cook¹,

Thomson²

2014

Aust Veterinary J

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The angularis oris axial pattern buccal flap is an option for reconstruction of large defects of the hard palate. Survival of the flap can be improved by removal of teeth likely to cause occlusal trauma to the flap's pedicle. In the event of distal flap necrosis, the repair may be salvaged by rotation of the remaining flap into the defect, following establishment of vascular supply from adjacent tissue.

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“…The lateral margin was 2 cm lateral from and parallel to the medial margin forming the 2 × 8 cm flap. This flap design is well established and produces a consistent necrosis of the distal half due to the flaps extended dimension [1, 16, 19, 20]. Perfusion of the abdominal region of the rat is similar to that of the human and thus this model can be modified to simulate perforator‐flaps (DIEP‐flap) and musculocutaneous flaps (TRAM).…”

Section: Methodsmentioning

confidence: 99%

Non‐viral VEGF₁₆₅ gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model

Holzbach¹,

Vlaskou²,

Neshkova³

et al. 2010

J Cellular Molecular Medi

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Adenoviral transduction of the VEGF gene in an oversized skin flap increases flap survival and perfusion. In this study, we investigated the potential of magnetofection of magnetic lipospheres containing VEGF165-cDNA on survival and perfusion of ischemic skin flaps and evaluated the method with respect to the significance of applied magnetic field and ultrasound. We prepared perfluoropropane-filled magnetic lipospheres (‘magnetobubbles’) from Tween60-coated magnetic nanoparticles, Metafectene, soybean-oil and cDNA and studied the effect in an oversized random-pattern-flap model in the rats (n= 46). VEGF-cDNA-magnetobubbles were administered under a magnetic field with simultaneously applied ultrasound, under magnetic field alone and with applied ultrasound alone. Therapy was conducted 7 days pre-operative. Flap survival and necrosis were measured 7 days post-operatively. Flap perfusion, VEGF-protein concentration in target and surrounding tissue, formation and appearance of new vessels were analysed additionally. Magnetofection with VEGF-cDNA-magnetobubbles presented an increased flap survival of 50% and increased flap perfusion (P < 0.05). Without ultrasound and without magnetic field, the effect is weakened. VEGF concentration in target tissue was elevated (P < 0.05), while underlying muscle was not affected. Our results demonstrate the successful VEGF gene therapy by means of magnetobubble magnetofection. Here, the method of magnetofection of magnetic lipospheres is equally efficient as adenoviral transduction, but has a presumable superior safety profile.

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Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model

Cited by 43 publications

References 38 publications

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Complications of the angularis oris axial pattern buccal flap for reconstruction of palatine defects in two dogs

Non‐viral VEGF₁₆₅ gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model

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Searching for the right timing of surgical delay: angiogenesis, vascular endothelial growth factor and perfusion changes in a skin-flap model

Cited by 43 publications

References 38 publications

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Vascular Endothelial Growth Factor (VEGF165) Plus Basic Fibroblast Growth Factor (bFGF) Producing Cells induce a Mature and Stable Vascular Network—a Future Therapy for Ischemically Challenged Tissue

Complications of the angularis oris axial pattern buccal flap for reconstruction of palatine defects in two dogs

Non‐viral VEGF165 gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model

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Product

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Non‐viral VEGF₁₆₅ gene therapy – magnetofection of acoustically active magnetic lipospheres (‘magnetobubbles’) increases tissue survival in an oversized skin flap model