Season‐long characterization of high‐cannabinoid hemp (Cannabis sativa L.) reveals variation in cannabinoid accumulation, flowering time, and disease resistance

Abstract

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Limited effect of environmental stress on cannabinoid profiles in high‐cannabidiol hemp (Cannabis sativa L.)

“…4.1 | Cannabinoid accumulation and ratios CBD, THC, and CBC accumulated during maturation of the inflorescence over the course the trial, as expected (Stack et al, 2021). Most plots sampled at week 3 (except herbicidetreated plots) had a total THC concentration >0.3%.…”

“…Adequate soil moisture was applied through trickle irrigation during periods with insufficient rainfall to maintain soil volumetric water content >0.27 m 3 m −3 . Temperature and rainfall data for this site are reported in Stack et al, (2021).…”

“…One shoot tip sample was collected by harvesting the top 10 cm of an upper canopy shoot from each of the three plants in a plot, and those three shoot tips were combined in a paper bag, airdried at room temperature, and milled in a Nutri Ninja Pro food blender (SharkNinja Operating LLC). Cannabinoids were extracted and quantified by high-pressure liquid chromatography (HPLC) using a previously established method (Stack et al, 2021). Total cannabinoids were calculated by summing the neutral form with the acidic form multiplied by a factor (0.877 for THCA, CBDA, and CBCA; 0.878 for CBGA) to account for decarboxylation.…”

“…Notably, CBDA synthase has been reported to synthesize approximately 5% THCA and 5% CBCA normalized to 100% CBDA (Zirpel et al, 2018). This is likely to be the primary source of the THCA and CBCA that have been detected in chemotype III hemp plants that do not express active THCA synthase (Stack et al, 2021;Toth et al, 2020). It is conceivable that allelic variation among CBDA synthases or expression of other cannabinoid synthases could lead to altered CBD:THC ratios.…”

Influence of Technological Maturity on the Secondary Metabolites of Hemp Concentrate (Cannabis sativa L.)

“…The difference in polyphenolic compounds could be caused by different types of cannabis plants. Cannabis is a highly diverse plant, and the content and metabolite profile differ between varieties [ 29 ]. In the study, the plants of species indica were tested, which could show a greater content of phenolic compounds.…”