Seasonal and substrate preferences of fungi colonizing leaves in streams: traditional versus molecular evidence

Nikolcheva, Liliya G.; Bärlocher, Feli×

doi:10.1111/j.1462-2920.2004.00709.x

Cited by 136 publications

(103 citation statements)

References 42 publications

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“…The effects of environmental variables on microbial communities are routinely evaluated to explain changes in biomass and community structure (44,70). Among physical variables, the role of temperature in controlling bacterial biomass on decaying litter is not consistent across studies, with some reporting significant temperature effects (70) and others reporting no effect of temperature (14).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Fungi, Bacteria, and Actinomycetes on Leaves Decomposing in a Stream

Das

Royer

Leff

2007

Appl Environ Microbiol

191

137

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Although fungi, bacteria, and specific bacterial taxa, such as the actinomycetes, have been studied extensively in various habitats, few studies have examined them simultaneously, especially on decomposing leaves in streams. In this study, sugar maple and white oak leaves were incubated in a stream in northeastern Ohio for 181 days during which samples were collected at regular intervals. Following DNA extraction, PCRdenaturing gradient gel electrophoresis (DGGE) was performed using fungus-, bacterium-, and actinomycetespecific primers. In addition, fungal and bacterial biomass was estimated. Fungal biomass differed on different days but not between leaves of the two species and was always greater than bacterial biomass. There were significant differences in bacterial biomass through time and between leaf types on some days. Generally, on the basis of DGGE, few differences in community structure were found for different leaf types. However, the ribotype richness of fungi was significantly greater than those of the bacteria and actinomycetes, which were similar to each other. Ribotype richness decreased toward the end of the study for each group except bacteria. Lack of differences between the two leaf types suggests that the microorganisms colonizing the leaf biofilm were primarily generalists that could exploit the resources of the leaves of either species equally well. Thus, we conclude that factors, such as the ecological role of the taxa (generalists versus specialists), stage of decay, and time of exposure, appeared to be more important determinants of microbial community structure than leaf quality.

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Section: Discussionmentioning

confidence: 99%

Diversity of Fungi, Bacteria, and Actinomycetes on Leaves Decomposing in a Stream

Das

Royer

Leff

2007

Appl Environ Microbiol

191

137

View full text Add to dashboard Cite

show abstract

“…This parameter could be used as a bioindicator of water quality in combination with species identification to discriminate between streams of low and high trophic statuses. However, seasonal fluctuations of aquatic hyphomycetes on decomposing leaves occur (30), and further studies are needed to assess the potential use of this group of fungi in water quality assessment.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, the study of bacterial diversity on decomposing leaves has been strongly restricted to a few cultivable bacteria (Ͻ1%). Molecular typing, such as denaturing gradient gel electrophoresis (DGGE) of a specific rRNA gene region, has proved useful for assessing diversity in both leaf-associated fungi and bacteria (7,8,9,11,30).…”

mentioning

confidence: 99%

Microbial Decomposer Communities Are Mainly Structured by Trophic Status in Circumneutral and Alkaline Streams

Duarte

Pascoal

Garabetian

et al. 2009

Appl Environ Microbiol

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In streams, the release of nitrogen and phosphorus is reported to affect microbial communities and the ecological processes they govern. Moreover, the type of inorganic nitrogen (NO 3 , NO 2 , or NH 4 ) may differently impact microbial communities. We aimed to identify the environmental factors that structure aquatic microbial communities and drive leaf litter decomposition along a gradient of eutrophication. We selected five circumneutral (Portuguese) and five alkaline (French) streams differing in nutrient concentrations to monitor mass loss of alder leaves, bacterial and fungal diversity by PCR-denaturing gradient gel electrophoresis, fungal biomass and reproduction, and bacterial biomass during 11 weeks of leaf immersion. The concentrations of inorganic nutrients in the stream water ranged from 5 to 300 g liter ؊1 soluble reactive phosphorus, 0.30 to 5.50 mg liter ؊1 NO 3 -N, 2 to 103 g liter ؊1 NO 2 -N, and <4 to 7,100 g liter ؊1 NH 4 -N. Species richness was maximum in moderately anthropized (eutrophic) streams but decreased in the most anthropized (hypertrophic) streams. Different species assemblages were found in subsets of streams with different trophic statuses. In both geographic areas, the limiting nutrient, either nitrate or phosphate, stimulated the microbial activity in streams of intermediate trophic status. In the hypertrophic streams, fungal biomass and reproduction were significantly lower, and bacterial biomass dramatically decreased at the site with the highest ammonium concentration. The limiting nutrients that defined the trophic status were the main factor structuring fungal and bacterial communities, whatever the geographic area. A very high ammonium concentration in stream water most probably has negative impacts on microbial decomposer communities.

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“…CAP, widely used for analysis of T-RFLP data (Nikolcheva and Barlocher, 2005;Gleeson et al, 2008), is a constrained ordination method, which also crossvalidates the model it derives.…”

Section: T-rflp Analysesmentioning

confidence: 99%

Possible interactions between bacterial diversity, microbial activity and supraglacial hydrology of cryoconite holes in Svalbard

et al. 2010

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The diversity of highly active bacterial communities in cryoconite holes on three Arctic glaciers in Svalbard was investigated using terminal restriction fragment length polymorphism (T-RFLP) of the 16S rRNA locus. Construction and sequencing of clone libraries allowed several members of these communities to be identified, with Proteobacteria being the dominant one, followed by Cyanobacteria and Bacteroidetes. T-RFLP data revealed significantly different communities in holes on the (cold) valley glacier Austre Brøggerbreen relative to two adjacent (polythermal) valley glaciers, Midtre Lové nbreen and Vestre Brøggerbreen. These population compositions correlate with differences in organic matter content, temperature and the metabolic activity of microbial communities concerned. No within-glacier spatial patterns were observed in the communities identified over the 2-year period and with the 1 km-spaced sampling. We infer that surface hydrology is an important factor in the development of cryoconite bacterial communities.

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Seasonal and substrate preferences of fungi colonizing leaves in streams: traditional versus molecular evidence

Cited by 136 publications

References 42 publications

Diversity of Fungi, Bacteria, and Actinomycetes on Leaves Decomposing in a Stream

Diversity of Fungi, Bacteria, and Actinomycetes on Leaves Decomposing in a Stream

Microbial Decomposer Communities Are Mainly Structured by Trophic Status in Circumneutral and Alkaline Streams

Possible interactions between bacterial diversity, microbial activity and supraglacial hydrology of cryoconite holes in Svalbard

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