Seasonal changes in ROS concentrations and sperm quality in unfrozen and frozen-thawed stallion semen

Mislei, Beatrice; Bucci, Diego; Malama, Eleni; Bollwein, H.; Mari, Gaetano

doi:10.1016/j.theriogenology.2019.12.016

Cited by 28 publications

(21 citation statements)

References 29 publications

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“…Sperm can be divided into high-and low-quality antifreeze sperm based on the quality of freeze-thawing, but the assessment methods of conventional sperm quality cannot be used to predict the antifreeze activity of fresh sperm before freezing [16,28]. Therefore, researchers have been trying to identify anti-freeze markers.…”

Section: Discussionmentioning

confidence: 99%

“…Data are presented as the means ± SE (n = 3). * P < 0.05, ** P < 0.01 VS the extension period of control group; # P < 0.05, ## P < 0.01 VS the thawed 30 min or thawed 240 min period of NAC group leading to oxidative stress [12,16]. Under high concentrations of ROS interference, the mitochondrial membrane potential is changed, the number of mitochondria is reduced, mitochondrial DNA is damaged, and the ATP levels are significantly reduced to cause cell apoptosis in sperm [32][33][34].…”

Section: Discussionmentioning

confidence: 99%

“…Uncontrolled (i.e. excessive) ROS generation can attack unsaturated fatty acids (UFAs) on the plasma membrane, alter mitochondrial membrane potential and induce protein sulfhydryl peroxidation, thereby altering sperm function and structure [13][14][15], which is regarded as an important cause of sperm damage during cryopreservation [16,17]. In addition, ROS could cause that the sperm be unable to fertilize the oocyte.…”

Section: Introductionmentioning

confidence: 99%

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Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Zhang

Wang

et al. 2021

BMC Vet Res

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Background Cryopreservation is an efficient way to store spermatozoa and is closely associated with the quality of sperm after the freeze-thaw process. During freeze-thaw cycling, excessive reactive oxygen species (ROS) are produced, and the effects of ROS on boar sperm during cryopreservation have not been identified. Results In this study, we evaluated the quality of boar spermatozoa in different steps of cryopreservation (extension, cooling, and thawing for 30 min and 240 min) with or without boar-sperm antioxidant (N-acetylcysteine (NAC)). The ROS levels, sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, ATP content, and sperm apoptosis were assayed. After thawing, the ROS level and sperm apoptosis were significantly increased, and the sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, and ATP content were significantly impaired compared with those at the extension period and cooling period. Moreover, the addition of N-acetyl L-cysteine (NAC) reversed these changes. Conclusion The freeze-thawing of boar spermatozoa impaired their motility, plasma membrane, mitochondrial activity, sperm chromatin structure and apoptosis by producing excessive ROS. Thus, the downregulation of ROS level by antioxidants, especially the NAC, is important for manufacturing frozen pig sperm to increase reproductive cells and livestock propagation, as well as to improve the application of frozen semen in pigs worldwide.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Zhang

Wang

et al. 2021

BMC Vet Res

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“…The effect of season on stallion reproduction has not been clearly established. Some authors reported that the non‐breeding season impairs endocrine profiles (Dhakal et al., 2011), number of Sertoli cells (Johnson, Varner, Tatum, & Scrutchfield, 1991), sexual behaviour characteristics, such as number of mounts per ejaculation and reaction time in the presence of an oestrous mare (Clay & Clay, 1992; Johnson, 1991; Picket, Faulkner, & Voss, 1975; Picket & Voss, 1972), and semen quality including semen volume, sperm concentration, total sperm number (Clay & Clay, 1992; Johnson, 1991; Picket et al, 1975; Picket & Voss, 1972), sperm kinetics (Jasko et al, 1991), total sperm number with progressive motility (Greiser, Sieme, Martinsson, & Distl, 2020), DNA fragmentation (Ortiz et al., 2020) and production of reactive oxygen species (Mislei, Bucci, Malama, Bollwein, & Mari, 2020). Other studies revealed lower DNA fragmentation and low intracellular calcium level, and higher total motility, viability (Wach‐Gygax et al., 2017) and progressive motility in the non‐breeding season (Greiser et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

“…In light horses, the best freezability was obtained in the non‐breeding season. Total and progressive motility, viability, ADN fragmentation, morphology and seminal plasma proteins related to fertility were superior in semen of stallions cryopreserved outside the breeding season (Greiser et al., 2020; Mislei et al., 2020; Wrench et al., 2010).…”

Section: Introductionmentioning

confidence: 99%

Semen quality and freezability analysis during breeding and non‐breeding seasons in heavy draft stallions in southern Chile

et al. 2020

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The aim of this study was to evaluate seasonal changes in basic parameters of sperm quality and freezability behaviour of ejaculates from 10 fertile heavy draft stallions. A total of 140 ejaculates were collected, processed and evaluated during both the breeding (September–November) and non‐breeding seasons (April–June). Fresh semen was evaluated for volume, concentration, total spermatozoa per ejaculate, plasma membrane integrity and total sperm motility. Cryopreserved samples were evaluated for plasma membrane integrity and sperm motility by the CASA system, and for the freezability index (FI), which was defined as the decreased ratio of viability after freezing–thawing. In fresh ejaculates, only viability showed significantly higher values in the breeding than in the non‐breeding season (64.0% ± 15.0% vs. 58.6% ± 12.0%, respectively; p < .05). The sperm post‐thawing analysis of viability and total motility parameters showed no significant changes linked to the season. However, the FI analysis showed that the ejaculates collected in the non‐breeding season had higher cryoresistance characteristics than those collected in the breeding season. Results suggest that the presence of some cryoprotective factor/s in heavy draft horse ejaculates could be modulated by seasonality, with higher protective effects in the non‐breeding season.

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Analysis of stallion spermatozoa metabolism using Agilent Seahorse XFp Technology

Ortiz-Rodriguez,

Bucci,

Tovar-Pascual

et al. 2024

Animal Reproduction Science

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Seasonal changes in ROS concentrations and sperm quality in unfrozen and frozen-thawed stallion semen

Cited by 28 publications

References 29 publications

Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Freeze-thawing impairs the motility, plasma membrane integrity and mitochondria function of boar spermatozoa through generating excessive ROS

Semen quality and freezability analysis during breeding and non‐breeding seasons in heavy draft stallions in southern Chile

Analysis of stallion spermatozoa metabolism using Agilent Seahorse XFp Technology

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