Seasonal changes in the nematode fauna in pine trees killed by the pinewood nematode, Bursaphelenchus xylophilus

Sriwati, Rina; Takemoto, Shuhei; Futai, Kazuyoshi

doi:10.3725/jjn.36.87

Cited by 14 publications

(14 citation statements)

References 13 publications

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“…In Sriwati et al . 's () experiment, PWN multiplied on Sphaeropsis sapinea as well as on Botritis cinerea . In contrast to this, little or no propagation was observed on the colonies of species of Trichoderma , Fusarium , Penicillium , Mucor , Mortierella , Gliocladium , Aspergillus , Rhizoctonia and Aureobasidium .…”

Section: Discussionmentioning

confidence: 94%

“…This might be attributed to the fact that the microbial environments have become unsuitable for Ophiostoma even when transmitted by Monochamus beetles (Sriwati et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…; Sriwati et al . ). Briefly, the sample preparations were placed on a metal net tray fitted inside of the funnel with paper tissue and then water was added to it until the wood was completely soaked.…”

Section: Methodsmentioning

confidence: 97%

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Occurrence of Bursaphelenchus mucronatus (Nematoda: Aphelenchoididae) and the microhabitat distribution of fungi in declining pine trees in a locale in Korea

Kim

et al. 2014

Entomological Research

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A total of 33 pine trees with symptoms of decline were collected in Jeonnam Province, South Korea, and were examined for the presence of nematodes. About 20% of the trees sampled were positive with Bursaphelenchus species. All Bursaphelenchus species were found in recently dead or dying trees. Based on morphological observations, the nematode extracted from the declining pine trees was identified as B. mucronatus. The highly pathogenic pine wood nematode B. xylophilus was not found in any pine trees sampled. B. mucronatus was easily reared on fungus Botrytis cinerea. Twenty one fungal isolates were isolated from dead trees, fallen twigs, and healthy pine trees. The fungal isolates belonged to Trichoderma genus and were dominant in the wood of partially declining pines. The blue-stain fungi transmitted by the Monochamus beetle were not detected. The B. mucronatus population decreased markedly on Auxarthron reticulatum DY-2 isolated from soils. The number of nematodes also reduced on Verticillium saksenae A-1, a nematophagous fungus, and Beauveria bassiana, an entomopathogenic fungus. This observation suggested the fungal production of nematicidal activity against B. mucronatus. When the fungal culture filtrates were also used for nematicidal activity on B. mucronatus, the culture filtrates of A-1, DY-2 and B. bassiana showed over 50% mortality within 48 h exposure. The fungi BC4, BC5 and BC6 isolated from declining pine trees inhibited the reproduction of B. mucronatus, and their culture filtrates also expressed nematicidal activity, indicating a possible interaction between the fungi in pine trees and nematodes at microhabitat level.

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Section: Discussionmentioning

confidence: 94%

“…This might be attributed to the fact that the microbial environments have become unsuitable for Ophiostoma even when transmitted by Monochamus beetles (Sriwati et al . ).…”

Section: Discussionmentioning

confidence: 97%

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Occurrence of Bursaphelenchus mucronatus (Nematoda: Aphelenchoididae) and the microhabitat distribution of fungi in declining pine trees in a locale in Korea

Kim

et al. 2014

Entomological Research

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“…Although many field surveys of nematodes have been conducted on dead pine trees in the Kyushu area (Akiba, unpubl. data), most of the dead trees examined suffered from pine wood nematodes, i.e., the nematode fauna of those trees was usually dominated by B. xylophilus, and the presence of other nematode species may be masked by this species (e.g., Sriwati et al, 2006). Therefore, further surveys of naturally dead pine trees, including other pine species, e.g., P. densiflora Sieb.…”

Section: Discussionmentioning

confidence: 97%

Bursaphelenchus osumiana n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead Pinus armandii var. amamiana in Osumi Islands in Japan

Kanzaki

Akiba

Kanetani³

et al. 2014

Nematol

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An undescribed Bursaphelenchus species was isolated and cultured from materials collected during a field survey of a declining endemic pine species. Two nematode isolates were obtained from dead Pinus armandii var. amamiana on two islands (Yakushima and Tanegashima) in Kagoshima, Japan. The new nematode is described and illustrated herein as B. osumiana n. sp. Typologically, B. osumiana n. sp. is similar to B. parvispicularis and B. paraparvispicularis, i.e., males of these species have a short, stout spicule. The new species is distinguished from the other two by the shape of the male bursal flap (very small and triangular) and female tail morphology (weakly ventrally arcuate with narrow rounded or weakly pointed terminus), which are unique to this species. Near full-length molecular sequences of the small subunit (18S), internal transcribed spacer region, and D2D3 expansion segments of the large subunit of ribosomal RNA and a partial sequence of the mitochondrial cytochrome oxidase subunit I (mtCOI) were obtained to molecularly profile the new species. The sequence profiles were identical for the two isolates of B. osumiana n. sp., and were close to those of B. parvispicularis and B. paraparvispicularis. The new species was clearly distinguished molecularly from both of its close relatives and its molecular phylogenetic status and typological characters were in accordance. Other biological characters, e.g., carrier insect species and pathogenicity with regard to pine tree species, have not been clarified and should be examined in future studies.

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“…Sriwati et al (2006) reported that the dominance of the pine wood nematode B. xylophilus in dead pine trees reduces population densities and species divergences of other nematode species. Hence, competition and exclusion occur between B. xylophilus and other nematodes that share the same habitat (deadwood).…”

Section: Discussionmentioning

confidence: 98%

Nematodes Associated WithDryocoetes uniseriatus(Coleoptera: Scolytidae)

Shimizu¹,

Tanaka²,

Akiba³

et al. 2013

Environ Entomol

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We examined the nematode fauna associated with a species of bark beetle, Dryocoetes uniseriatus Eggers, as part of a biodiversity survey of forest beetle-associated nematodes. Collections were made in a pine stand at an experimental forest station in Ibaraki, Japan, from April to July of 2011; we examined the nematode association in 273 insects collected during this time. In total, 68% of the insects were associated with at least one species of nematode. Six species of nematodes, including two phoretic microbe feeders (Bursaphelenchus rainulfi Braasch & Burgermeister and Micoletzkya sp.), one insect parasite and nematode predator (Devibursaphelenchus cf. eproctatus), one insect parasite (Contortylenchus sp.), one insect parasite and potential microbe feeder (unidentified rhabditid parasite), and one potential insect parasite and fungal feeder (B. sinensis) were recovered from the beetles. D. cf. eproctatus was enclosed in nematangia on the backsides of the elytra, B. rainulfi was isolated from the backsides of the elytra or enclosed in nematangia, Micoletzkya sp. was isolated from under the elytra, Contortylenchus sp. and a rhabditid parasite parasitized the body cavity, and B. sinensis was found in the digestive tract of the insect. The association patterns of the nematode species varied seasonally, although definitive interactions among species (e.g., segregation, competition) were not observed.

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Seasonal changes in the nematode fauna in pine trees killed by the pinewood nematode, Bursaphelenchus xylophilus

Cited by 14 publications

References 13 publications

Occurrence of Bursaphelenchus mucronatus (Nematoda: Aphelenchoididae) and the microhabitat distribution of fungi in declining pine trees in a locale in Korea

Occurrence of Bursaphelenchus mucronatus (Nematoda: Aphelenchoididae) and the microhabitat distribution of fungi in declining pine trees in a locale in Korea

Bursaphelenchus osumiana n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead Pinus armandii var. amamiana in Osumi Islands in Japan

Nematodes Associated WithDryocoetes uniseriatus(Coleoptera: Scolytidae)

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