Seasonal changes of sperm storage and correlative structures in male and female soft-shelled turtles, Trionyx sinensis

Han, Xiuxin; Li, Zhang; Li, Meiying; HuiJun, Bao; Hei, Nainan; Chen, Qiusheng

doi:10.1016/j.anireprosci.2007.09.011

Cited by 22 publications

(17 citation statements)

References 28 publications

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“…While in reptiles, Esponda (1991) suggested that capacitation is not a pre-requisite for fertilization and some testicular spermatids are able to fertilize the egg. Previous work of our group (Xiangkun et al, 2008) reported that immature-like spermatozoa could be observed in the oviduct in female soft-shelled turtle. The spermatozoa discharged into the epididymis, in general show a narrow and elongated shape, only a small amount of cytoplasmic droplets attached to the midpiece or other position.…”

Section: Discussionmentioning

confidence: 93%

The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season

Xin-min

Gandahi

Liu

et al. 2013

Micron

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Section: Discussionmentioning

confidence: 93%

The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season

Xin-min

Gandahi

Liu

et al. 2013

Micron

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“…These characteristics for this region of the state of Maranhao were equal to those observed by Pereira et al (2007), corroborated by Chaves (2011). Xiangkun et al (2008) studied the turtles Trionyx sinensis and found out that on summer, i.e. the breeding period, one could observe a large number of principal cells in the epididymis, besides glycogen granules with lipids in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Morphological study of epididymides in the scorpion mud turtle in natural habitat (Kinosternon scorpioides – Linnaeus, 1976)

et al. 2013

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ResumoEstudo morfológico dos epidídimos de jurará em habitat natural (Kinosternon scorpioides -Linnaeus, 1976). No estado do Maranhão, é encontrado o quelônio de água doce Kinosternon scorpioides, conhecido como jurará, que possui valor social, econômico e ambiental. Vinte jurarás adultos foram coletados nos meses de março, junho, setembro e dezembro, correspondendo aos dois períodos do ano: as estações chuvosa e seca. De cada animal colheram-se os epidídimos para averiguar a existência de sazonalidade reprodutiva. Os órgãos foram avaliados por microscopia de luz e eletrônica de varredura e de transmissão e analisados à altura do epitélio epididimário e os diâmetros tubular e luminal. Os epidídimos estavam divididos em rede testis, ducto eferente e ducto epididimário. Na estação chuvosa, eles apresentaram, epitélio pseudoestratificado estereociliado; na estação seca, eles se caracterizaram por células simples, cúbicas e não ciliadas. A morfometria dos diâmetros tubular e luminal e a altura epitelial do epidídimo apresentaram momentos de alternância epitelial ao longo dos períodos estudados, com as maiores médias na estação chuvosa, no período reprodutivo. Os espermatozoides foram observados durante o ano todo, apesar de sua viabilidade não poder ser avaliada. Tendo em vista os dados obtidos, pode-se afirmar que o jurará apresenta sazonalidade reprodutiva.Palavras-chave: Fauna silvestre; Morfometria epididimária; Reprodução; Sazonalidade reprodutiva AbstractIn the state of Maranhao, Brazil, one can find the freshwater chelonian Kinosternon scorpioides, known as scorpion mud turtle, which has social, economic, and environmental value. Twenty adult scorpion mud turtles were collected in the months of March, June, September, and December, corresponding to the two periods of the year: the rainy and dry seasons. The epididymides were collected from each animal to check the existence of reproductive seasonality. The organs were evaluated through light, scanning electron, and transmission microscopy and analyzed at the epididymal epithelium height and the tubular and luminal diameters. The epididymides were divided into rete testis, ductuli efferentes, and ductus epididymis. In the rainy season, they presented stereo ciliated pseudostratified epithelium; in the dry season, they were characterized by simple, cubic, and non-ciliated cells. The morphometry of the tubular and luminal diameters and the epididymal epithelium height showed moments of epithelial alternation over the studied periods, with the highest averages in the rainy season, within the reproductive period. Spermatozoids were observed throughout the year, although their viability couldn't be evaluated. Taking into account the data obtained, one may claim that the scorpion mud turtle presents reproductive seasonality.

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“…There is a separation of gametogenesis, copulation, and fertilization in this animal. Consequently, sperm storage ensures successful fertilization, and occurs in both the male epididymis (Xiangkun et al, ; Bian et al, ) and the female reproductive tract, including the oviduct (Han et al, ; Xiangkun et al, ). The oviduct of P. sinensis can be divided into five distinct regions: the vagina, uterus, isthmus, uterine tube, and infundibulum (Ullah et al, ).…”

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confidence: 99%

B‐Cell Lymphoma‐2 Localization in the Female Reproductive Tract of the Chinese Soft‐Shelled Turtle, Pelodiscus Sinensis and Its Relationship With Sperm Storage

Yuan

Chen

et al. 2015

The Anatomical Record

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The aim of the present study was to investigate the expression and localization of B-cell lymphoma-2 (Bcl-2) in the oviduct of the Chinese soft-shelled turtle, Pelodiscus sinensis, during the reproductive cycle to analyze the relationship between Bcl-2 and sperm storage. Bcl-2 expression was confirmed in the P. sinensis oviduct by western blot analysis. Hematoxylin-eosin staining showed that female P. sinensis stored sperm from November to April of the following year. The oviduct showed positive immunostaining for Bcl-2 of epithelial ciliated cells, gland ducts, and gland cells. Bcl-2 expression in the oviduct was associated with sperm storage occurrence. This indicates that the survival factor Bcl-2 may play a role in P. sinensis sperm storage.

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Seasonal changes of sperm storage and correlative structures in male and female soft-shelled turtles, Trionyx sinensis

Cited by 22 publications

References 28 publications

The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season

The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season

Morphological study of epididymides in the scorpion mud turtle in natural habitat (Kinosternon scorpioides – Linnaeus, 1976)

B‐Cell Lymphoma‐2 Localization in the Female Reproductive Tract of the Chinese Soft‐Shelled Turtle, Pelodiscus Sinensis and Its Relationship With Sperm Storage

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