Seasonal dynamics in leaf viromes of grapevines depicting leafroll syndrome under tropical condition

Sidharthan, V. Kavi; Sevanthi, Amitha Mithra; Venkadesan, Sureshkumar; Diksha, Damini; Baranwal, V. K.

doi:10.1007/s40858-022-00524-x

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…Metatranscriptomic analysis can reveal the diversity and abundance of total viromes and is commonly used to explore viromes among different host species, geographical distributions, seasonal environments, and sampling years ( 34 , 49 – 52 ). Interestingly, in this study, ticks from different sampling locations carried the same viruses, despite the great distance between the samples ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Metatranscriptomics Reveals the Diversity of the Tick Virome in Northwest China

et al. 2022

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Ticks are widely distributed in pastoral areas in northwestern China and act as vectors that carry and transmit a variety of pathogens, especially viruses. Our study revealed the diversity of tick viruses in Xinjiang and Inner Mongolia and uncovered the phylogenetic relationships of some RNA viruses, especially the important zoonotic tick-borne severe fever with thrombocytopenia syndrome virus in Inner Mongolia.

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Section: Discussionmentioning

confidence: 99%

Metatranscriptomics Reveals the Diversity of the Tick Virome in Northwest China

et al. 2022

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“…In addition to fungal and bacterial diseases, Indian vineyards have documented the presence of viral pathogens responsible for causing GLD and Rupestris Stem Pitting disease (RSP). These viral diseases have been studied extensively, as indicated by various research references (Kumar et al, 2012a, b, c;Kumar et al, 2013;Kumar et al, 2017;Rai et al, 2018;Rai et al, 2021;Sidharthan et al, 2020Sidharthan et al, , 2022.…”

Section: Introductionmentioning

confidence: 99%

“…Detection is the foremost step in devising disease management strategies for plant pathogens. In India, immuno-based diagnostics have been developed for three species of grapevine viruses viz., GLRaV-1 (Kumar et al, 2012b;Kumar et al, 2018), GLRaV-3 (Kumar et al, 2015;2018;Sidharthan et al, 2020Sidharthan et al, , 2022 and GLRaV-4 (Rai et al, 2018). Although 86 viruses are known to infect grapevine worldwide (Martelli, 2018;Fuchs, 2020), the preparedness for diagnosis of grapevine viruses exists hardly for a few viruses in India.…”

Section: Introductionmentioning

confidence: 99%

“…In the recent past, virome analysis through High Throughput Sequencing (HTS) was carried out to determine the presence of viruses in the samples collected from Indian grapevine vineyards. This work revealed the presence of five grapevine viruses viz., GLRaV-3, grapevine virus L (GVL), GRSPaV, grapevine geminivirus A (GVA), Grapevine Polerovirus 1 (GPoV-1), in the Indian vineyards (Sidharthan et al, 2020(Sidharthan et al, , 2022.…”

Section: Introductionmentioning

confidence: 99%

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Present Status of Viral Diseases of Grapevine (<i>Vitis Vinifera</i> L.) and their Management Strategies in India

Holkar,

Markad,

Sharma

et al. 2024

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Grapevine is an important fruit crop cultivated in temperate, subtropical and tropical conditions in India. The maximum share in area, production, and productivity of grapes is governed by four states viz. Maharashtra, Karnataka, Tamil Nadu and Andhra Pradesh. The sudden and drastic changes in climatic conditions and the emergence of diseases have made grapevine cultivation more challenging. Fungal and bacterial diseases are the major constraints in the grapevine production. Besides, some viral diseases like Grapevine Leafroll Disease (GLD) and Rupestris Stem Pitting Associated Diseases (RSPD) are reported in Indian vineyards. Although the presence of Grapevine Leafroll-Associated Viruses (GLRaVs) viz., GLRaV-1, GLRaV-3, and GLRaV-4 are known the impact on yield and quality of the grapes is not yet studied. Similarly, the Rupestris Stem Pitting Associated Virus (RSPaV) is known to infect the Indian vineyards of different locations but a complete understanding of their overall impact on vineyards is lacking. Therefore, it is presumed that fewer or no losses occur due to GLD and RSPD diseases. There is a strong need for hours to study the impact of known viruses on physiological and yield contributing parameters. In addition to this, robust, rapid and reliable diagnostic techniques are required for the detection of known and unknown viruses. The presence of new and emerging viruses in the Indian vineyards cannot be overlooked. Further, for the management of the viruses and avoiding their spread, the availability of Disease-Free Quality Planting Material (DFQPM) is a prerequisite. To produce DFQPM, various tissue culture techniques need to be standardized for the elimination of the virus/es from the diseased vines. The Tissue Culture-Raised Quality Planting Material (TCQPM) needs to be further tested for genetic purity (true-to-type) and virus indexing to confirm DFQPM. By considering the importance of DFQPM, recently, the National Horticulture Board (NHB), Govt. of India in association with the Asian Development Bank (ADB) has initiated a National Mission on Clean Plant Programme. The implementation of this initiative is more challenging and expected to open different avenues of research and development in the production of DFQPM for the growers. This is an important and timely beginning of the production of DFQPM.

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“…Detection of grapevine viruses becomes crucial to control their spread and reduce the subsequent impact on quality and yield. Several molecular (RT-PCR, q-PCR) and next-generation sequencing (NGS)-based techniques have been employed to efficiently detect these viruses ( Sidharthan et al., 2022 ). However, isothermal amplification-based detection assays such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) are becoming popular for targeted detection due to their sensitivity, specificity, and attribute of not requiring stringent thermal cycling ( Sharma et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian grapevine cultivars

et al. 2023

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Geminiviruses are known to infect several fields and horticultural crops around the globe. Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then, it has been reported in several countries. The complete genome recovered through high-throughput sequencing (HTS)-based virome analysis in Indian grapevine cultivars had all of the six open reading frames (ORFs) and a conserved nonanucleotide sequence 5′-TAATATTAC-3′ similar to all other geminiviruses. Recombinase polymerase amplification (RPA), an isothermal amplification technique, was developed for the detection of GGVA in grapevine samples employing crude sap lysed in 0.5 M NaOH solution and compared with purified DNA/cDNA as a template. One of the key advantages of this assay is that it does not require any purification or isolation of the viral DNA and can be performed in a wide range of temperatures (18°C–46°C) and periods (10–40 min), which makes it a rapid and cost-effective method for the detection of GGVA in grapevine. The developed assay has a sensitivity up to 0.1 fg μl-1 using crude plant sap as a template and detected GGVA in several grapevine cultivars of a major grapevine-growing area. Because of its simplicity and rapidity, it can be replicated for other DNA viruses infecting grapevine and will be a very useful technique for certification and surveillance in different grapevine-growing regions of the country.

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Seasonal dynamics in leaf viromes of grapevines depicting leafroll syndrome under tropical condition

Cited by 7 publications

References 21 publications

Metatranscriptomics Reveals the Diversity of the Tick Virome in Northwest China

Metatranscriptomics Reveals the Diversity of the Tick Virome in Northwest China

Present Status of Viral Diseases of Grapevine (<i>Vitis Vinifera</i> L.) and their Management Strategies in India

Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian grapevine cultivars

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