Seasonal Effect on the Biological Activities of Litsea glaucescens Kunth Extracts

Abstract

This study shows the seasonal effect on the antioxidant, antiproliferative, and antimicrobial activities of L. glaucescens Kunth (LG) leaves extracts. Their antioxidant activity was evaluated through the DPPH, FRAP, and ORAC assays. Their phenolic content (PC) was determined by means of the Folin-Ciocalteu method, and the main phenolic compounds were identified through a HPLC-DAD analysis. Antiproliferative activity was determined by MTT assay against HeLa, LS 180, M12.C3.F6, and ARPE cell lines. Antimicrobial potential was evaluated against Staphylococcus aureus and Escherichia coli using a microdilution method. All the LG extracts presented high antioxidant activity and PC, with quercitrin and epicatechin being the most abundant. Antioxidant activity and PC were affected by the season; particularly autumn (ALGE) and summer (SULGE) extracts exhibited the highest values (p < 0.05). All extracts presented moderate antiproliferative activity against the cell lines evaluated, HeLa being the most susceptible of them. However, ALGE and SULGE were the most active too. About antimicrobial activity, SULGE (MIC90 < 800 μg/mL; MIC50 < 400 μg/mL), and SLGE (MIC50 < 1000 μg/mL) showed a moderate inhibitory effect against S. aureus. These findings provide new information about the seasonal effect on the PC and biological properties of LG extracts. Clearly, antioxidant activity was the most important with respect to the other two.

Biological Activities of Extracts from Different Parts of two Cultivars of Prunus persica ‘Akatsuki’ and ‘Fastigiata’

“…In the same way, the ethanol extract of buds collected from 'Fastigiata' showed a higher value than that of the buds collected from 'Akatsuki' (about 2.5-fold greater). Antioxidant activity of extracts from natural products are vary depend on the cultivar [12], the condition of growth such as soil and seasons [13,14]. In our study, sampling seasons were same between 'Akatsuki' and 'Fastigiata'.…”

Litsea glaucescens Kuth possesses bactericidal activity against Listeria monocytogenes

“…This result agrees with other works where alcoholic extracts from this plant have shown antibacterial activity. Indeed, a methanolic extract of L. glaucescens leaves possess activity in vitro against Escherichia coli and Staphylococus aureus (CMI ≥ 1 and 0.8 mg/mL, respectively) ( Meckes et al, 1995 ; López-Romero et al, 2018 ). Furthermore, Cruz et al (2014) determined the MIC values of an ethanolic extract of L. glaucenses against Bacillus subtilis ATCC6051 (0.16 mg/mL), Mycobacterium smegmatis ATCC607 (0.62 mg/mL), and E. coli (0.62 mg/mL).…”

“…Due to its extensive use, L. glaucescens is one of the main non-timber forest products in México ( Guzmán-Gutiérrez et al, 2012 ; Dávila-Figueroa et al, 2016 ). Several researchers have evaluated the antihypertensive, antidepressant, antioxidant, and antibacterial activities of this plant ( Meckes et al, 1995 ; Guzmán-Gutiérrez et al, 2012 ; Cruz et al, 2014 ; Guzmán Gutiérrez, Reyes Chilpa & Bonilla Jaime, 2014 ; Gamboa-Gómez et al, 2016 ; Medina-Torres et al, 2016 ; López-Romero et al, 2018 ; Shi, Zhang & Guo, 2018 ; López-Romero et al, 2022 ). Regarding its antibacterial activities, studies have demonstrated the activity of this plant against some Gram-positive and Gram-negative bacteria, but its activity against L. monocytogenes has not been evaluated until now.…”

Phenolic Composition and Biological Properties of Rhus microphylla and Myrtillocactus geometrizans Fruit Extracts

“…The anticancer activity of these compounds is caused by the promotion and generation of reactive oxygen species and the arrest of the cell cycle, which induces apoptosis; furthermore, the synergism between compounds can improve their bioactivities [ 34 , 35 ]. López-Romero [ 36 ] identified the presence of epicatechin in extracts of Litsea glaucescens , having antiproliferative effect against HeLa cancer cell line (IC 50 of 45.80 µg/mL) and selectivity between cancerous and noncancerous cell lines. Similarly, Rm and Mg fruit extracts did not show cytotoxicity against the noncancerous ARPE-19 cell line; in addition, three extracts showed selectivity (RmH-C, RmH-OH5, and RmH-OH10) by presenting moderate activity against HeLa.…”

“…The FRAP assay involves the ability of extract to reduce ferric ions (Fe 3+ ) [ 45 ]; the ferric reducing ability was evaluated according to the microplate-adapted methodology described by Lopéz-Romero [ 36 ]. The working solution of FRAP was made mixing 10 volumes of 300 mM acetate buffer (pH 3.6), 1 volume of 20 mM aqueous ferric chloride, and 1 volume of 40 mM TPTZ (HCl 40 mM as solvent).…”