Seasonal expressions of GPR41 and GPR43 in the colon of the wild ground squirrels (&lt;em&gt;Spermophilus dauricus&lt;/em&gt;)

Yang, Xiaoying; Liu, Xuhao; Song, Fengcheng; Hao, Wei; Gao, Feng; Zhang, Haolin; Han, Yingying; Wang, Qiang; Yuan, Zhengrong

doi:10.4081/ejh.2022.3351

Cited by 4 publications

(5 citation statements)

References 41 publications

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“… 77 , 81 GPR41 and GPR43, when expressed in the adipose tissue, function to stimulate leptin secretion and serves to suppress insulin signaling and adipogenesis, respectively ( Figure 4C ). 87 , 102 , 103 Acetate and propionate are notified to induce adipogenicity through GPR43. 44 Therefore GPR41/GPR43 signaling mediated by SCFAs has been shown to modulate insulin secretion directly in pancreatic β cells.…”

Section: Scfas-metabolic Pathologies Axismentioning

confidence: 99%

The Implication of Short-Chain Fatty Acids in Obesity and Diabetes

et al. 2023

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Evidence indicates that short-chain fatty acids (SCFAs) generated from the gut microbiota play crucial roles in host metabolism. They contribute to metabolic regulation and energy acquisition of the host by influencing the development of metabolic disorders. This review aims to synthesize recent advances from the literature to investigate the implication of SCFAs in the modulation of obesity and diabetes pathologies. For a better understanding of the relationships between SCFAs and host metabolism, we need to answer some questions: What is the biochemistry of SCFAs, and how they are generated by gut microbiota? What are the bacteria producing of SCFAs and from which routes? How SCFAs are absorbed and transported in the gut by different mechanisms and receptors? How SCFAs involved in obesity and diabetes pathologies?

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Section: Scfas-metabolic Pathologies Axismentioning

confidence: 99%

The Implication of Short-Chain Fatty Acids in Obesity and Diabetes

et al. 2023

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“…Previously, GPR41 and GPR43 have been reported to participate in the regulation of lipid metabolism ( 20 , 21 ). However, it is still not clear the biological role of GPR41 and GPR43 in milk fat synthesis.…”

Section: Introductionmentioning

confidence: 99%

Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway

Zheng²,

Liu³

et al. 2023

Front. Nutr.

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BackgroundFat is a critical component in milk, which provided energy for the early growth and development of mammals. Milk fat is positively related to the concentration of acetate in the blood, while the underlying mechanism is still unclear.ObjectiveThis study is to investigate the effects of sodium acetate (NaAc) on milk fat synthesis in the mammary gland, and explored the underlying mechanism.MethodsIn vitro experiments were carried out in mouse mammary epithelial cell line (HC11) cells cultured with NaAc to explore the potential pathway of NaAc on milk fat synthesis. Furthermore, 24 pregnant mice (from d 18.5 of gestation to d 7 of lactation, exposed to 200 mM NaAc drinking water) were used as an in vivo model to verify the results.ResultsIn this study, we found that NaAc promoted milk fat synthesis and the expression of related genes and proteins in HC11 mammary epithelial cells with the activation of GPCR and mTORC1 signaling pathways (p < 0.05). Pretreatment with the mTORC1 inhibitors and G protein inhibitors attenuated the NaAc-induced milk fat synthesis in HC11 mammary epithelial cells (p < 0.05). Importantly, the effect of NaAc on milk synthesis was attenuated in GPR41 and GPR43 knockdown HC11 mammary epithelial cells (p < 0.05). This evidence indicates that NaAc might regulate milk fat synthesis through the GPR41/GPR43-mTORC1 pathway. Consistently, in in vivo experiment, dietary supplementation with NaAc significantly increased milk fat content and fat synthesis-related proteins in mice mammary glands with the activation of mTORC1 and GPCR signaling pathways at peak lactation (p < 0.05).ConclusionThe addition of NaAc promoted the increase of milk fat synthesis in HC11 mammary epithelial cells and mice mammary glands at peak lactation. Mechanistically, NaAc activates GPR41 and GPR43 receptors, leading to the activation of the mTORC1 signaling pathway to promote the synthesis of milk fat.

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“…Wild Daurian ground squirrels also show some seasonal differences in their gut [ 35 ]. Previously, we found that the important SCFA receptors G-protein-coupled receptor 41 (GPR41) and G-protein-coupled receptor 43 (GPR43) in the wild Daurian ground squirrels were seasonally expressed in the colon [ 36 ]. In prior research, we used 16s rRNA sequencing to initially analyze the gut microbiota composition of wild Daurian ground squirrels [ 37 ], however, we did not use metagenomic sequencing to examine this issue.…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variation in Gut Microbiota of the Wild Daurian Ground Squirrel (Spermophilus dauricus): Metagenomic Insights into Seasonal Breeding

Song

Zhang

et al. 2023

Animals

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The Spermophilus dauricus, the wild Daurian ground squirrel, is known to exhibit seasonal breeding behavior. Although the importance of gut microbiota in animal digestion, metabolism, and immunity is well-established, the correlation between gut microbiota and seasonal breeding in this species remains inadequately explored. In the present study, using metagenomic sequencing technology, the compositions and functions of the gut microbiota of wild Daurian ground squirrels in different breeding seasons were explored. The dominant gut microbial phyla were Firmicutes and Bacteroidetes. The Firmicutes were predominant in the breeding season, whereas Bacteroidetes were predominant in the non-breeding season. At the genus level, Lactobacillus accumulated during the breeding season, whereas Odoribacter and Alistipes increased during the non-breeding season. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genome) annotations indicated that genes in gut samples were highly associated with metabolic functions. The differential expression gene analysis showed that genes related to the phosphotransferase system, cysteine, and methionine metabolism were highly expressed during the breeding season, whereas the non-breeding season upregulated genes were enriched in starch and sucrose metabolism and bacterial chemotaxis pathways. In conclusion, this study could provide a reference for investigating gut microbiota in seasonal breeding animals and offer new insight into gut microbial function.

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Seasonal expressions of GPR41 and GPR43 in the colon of the wild ground squirrels (<em>Spermophilus dauricus</em>)

Cited by 4 publications

References 41 publications

The Implication of Short-Chain Fatty Acids in Obesity and Diabetes

The Implication of Short-Chain Fatty Acids in Obesity and Diabetes

Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway

Seasonal Variation in Gut Microbiota of the Wild Daurian Ground Squirrel (Spermophilus dauricus): Metagenomic Insights into Seasonal Breeding

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