Seasonal Incidence and Molecular Characterization of<i>Salmonella</i>from Dairy Cows, Calves, and Farm Environment

Pangloli, Philipus; Dje, Yobouet; Ahmed, Omaima; Doane, C.A.; Oliver, S. P.; Draughon, F. A.

doi:10.1089/fpd.2007.0048

Cited by 56 publications

(41 citation statements)

References 20 publications

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“…The composition of airborne or settled dust is complex and is known to be subject to daily and seasonal changes (20). Some studies highlight the relationships between exposure to FIG.…”

Section: Discussionmentioning

confidence: 99%

“…Our results also corroborated this conclusion, since we reported a lower correlation between settled dust and individual air sampling results than the correlation obtained when mean values were considered. This poor correlation between air sampling and settled-dust methods obtained when each farm was considered separately may therefore be indicative of the high variability of the microbial composition of airborne samples over time, even during the same day (20,31). For instance, hay distribution resulted in increases in the levels of Eurotium and Cladosporium, molds known to use hay as a substrate for growth.…”

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confidence: 99%

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Assessment of Dust Sampling Methods for the Study of Cultivable-Microorganism Exposure in Stables

Normand

Vacheyrou

Súdre

et al. 2009

Appl Environ Microbiol

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Studies have shown a link between living on a farm, exposure to microbial components (e.g., endotoxins or ␤-D-glucans), and a lower risk for allergic diseases and asthma. Due to the lack of validated sampling methods, studies of asthma and atopy have not relied on exposure assessment based on culture techniques. Our objective was therefore to compare several dust sampling methods for the detection of cultivable-microorganism exposure in stables. Sixteen French farms were sampled using four different methods: (i) active air sampling using a pump, (ii) passive dust sampling with a plastic box, (iii) dust sampling with an electrostatic dust fall collector (wipe), and (iv) dust sampling using a spatula to collect dust already settled on a windowsill. The results showed that collection of settled dust samples with either plastic boxes or wipes was reproducible (pairwise correlations, 0.72 and 0.73, respectively) and resulted in highly correlated results (pairwise correlation between the two methods, 0.82). We also found that settled dust samples collected with a plastic box correctly reflected the composition of the samples collected in the air of the stable when there was no farmer activity. A loss of microbial diversity was observed when dust was kept for 3 months at room temperature. We therefore conclude that measurement of viable microorganisms within a reasonable time frame gives an accurate representation of the microbial composition of stable air.

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“…The composition of airborne or settled dust is complex and is known to be subject to daily and seasonal changes (20). Some studies highlight the relationships between exposure to FIG.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Assessment of Dust Sampling Methods for the Study of Cultivable-Microorganism Exposure in Stables

Normand

Vacheyrou

Súdre

et al. 2009

Appl Environ Microbiol

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“…Salmonella, in particular, is one of the most common pathogens in the European Union (Pangloli et al, 2008). Although its diffusion has been steadily decreasing, in 2008 salmonellosis remained, behind campylobacteriosis, the second most commonly reported human zoonoses, accounting for 131,468 confirmed human cases in 27 EU countries, with a notification rate of 26.4 per 100,000 inhabitants (European Food Safety Authority [EFSA], 2010).…”

Section: Introductionmentioning

confidence: 99%

Are you cooking your meat enough? The efficacy of the Theory of Planned Behavior in predicting a best practice to prevent salmonellosis

Mari

Tiozzo

Capozza

et al. 2012

Food Research International

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“…Besides the high morbidity and mortality rates in food-borne salmonellosis, food recalls and withdrawals are known to cause significant economical losses in the food industry (22). European baseline studies of [2005][2006] reports Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Temelli¹,

Kahya²,

Ata³

et al. 2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standard culture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCycler PCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year period from 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layer production. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performed according to ISO 6887-4:2003, ISO 6579 and LCPCR, respectively. Overall Salmonella detection rate by ISO and LCPCR were 15.8 % (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, and in 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %) shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. These findings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routine monitoring by rapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health.

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Seasonal Incidence and Molecular Characterization ofSalmonellafrom Dairy Cows, Calves, and Farm Environment

Cited by 56 publications

References 20 publications

Assessment of Dust Sampling Methods for the Study of Cultivable-Microorganism Exposure in Stables

Assessment of Dust Sampling Methods for the Study of Cultivable-Microorganism Exposure in Stables

Are you cooking your meat enough? The efficacy of the Theory of Planned Behavior in predicting a best practice to prevent salmonellosis

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

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