Seasonal structural and functional changes in the photosynthetic apparatus of evergreen conifers

Maslova, T. G.; Mamushina, N. S.; Sherstneva, O. A.; Bubolo, L. S.; Zubkova, Elena

doi:10.1134/s1021443709050045

Cited by 23 publications

(13 citation statements)

References 20 publications

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“…In agreement with previous works (Stefanowska et al 2002, Maslova et al 2009, Muller et al 2009), pronounced and reversible modifications in the ultrastructure of leaf cells and chloroplasts occurred during winter de‐acclimation. Our results comprise evidence of this postulated hypothesis since ultrastructural changes were found when winter‐acclimated and recovered leaves were compared (Fig.…”

Section: Discussionsupporting

confidence: 93%

Patterns of spatio‐temporal distribution of winter chronic photoinhibition in leaves of three evergreen Mediterranean species with contrasting acclimation responses

Silva-Cancino

Esteban

Artetxe

et al. 2012

Physiologia Plantarum

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High irradiance and relatively low temperature, which characterize Mediterranean winters, cause chilling stress in plants. Downregulation of photosynthetic efficiency is a mechanism that allows plants to survive these conditions. This study aims to address whether this process shows a regular spatial pattern across leaf surface or not. Three species (Buxus sempervirens, Cistus albidus and Arctostaphylos uva-ursi) with contrasting responses to winter stress were studied. During 7 days, macro and micro Fv/Fm spatial patterns were monitored by the use of chlorophyll fluorescence imaging techniques. In the field, the strongest photoinhibition was found in B. sempervirens, while there was almost no chronic photoinhibition in C. albidus. In leaves of the first species, Fv/Fm decreased from base to tip while in C. albidus it was uniform over the leaf lamina. An intermediate behavior is shown by A. uva-ursi leaves. Spatial heterogeneity distribution of Fv/Fm was found inside the leaves, resulting in greater Fv/Fm values in the inner layers than in the outer ones. Neither xanthophyll-linked downregulation of Fv/Fm nor protein remobilization were the reasons for such spatial patterns since pigment composition and nitrogen content did not reveal tip-base differences. During recovery from winter, photoinhibition changes occurred in Fv/Fm, pigments and chloroplast ultrastructure. This work shows for the first time that irrespective of physiological mechanisms responsible for development of winter photoinhibition, there is an acclimation response with strong spatio-temporal variability at leaf level in some species. This observation should be taken into account when modeling or scaling up photosynthetic responses.

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Section: Discussionsupporting

confidence: 93%

Patterns of spatio‐temporal distribution of winter chronic photoinhibition in leaves of three evergreen Mediterranean species with contrasting acclimation responses

Silva-Cancino

Esteban

Artetxe

et al. 2012

Physiologia Plantarum

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“…There is evidence that the pro cess of Vx deepoxidation is constrained in a feedback manner by the production of Zx or by rearrangements of lamellar membranes [48]. Heterogeneity was also observed for Zx, a second VC component: a part of Zx content did not participate in the backward reaction of the cycle [58]. At the same time, the secondary caro tenoid rhodoxanthin, produced upon nonenzymatic oxidation of Zx, was accumulated.…”

Section: Structual-functional Organization and Heterogeneity Of Violamentioning

confidence: 97%

Current views on the function of the violaxanthin cycle (development of ideas put forward by D.I. Sapozhnikov)

Maslova

Markovskaya

2012

Russ J Plant Physiol

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David Iosifovich Sapozhnikov (17.06.1911-23.11.1983), a well known plant physiologist and evolutionist would have been 100 this year. Sapozhnikov investigated the role of carotenoids in plant life and acquired worldwide recognition for his discovery of the violaxanthin cycle. This review considers the most important Sapozhnikov's results and hypotheses elaborated by subsequent research, as well as the modern concepts in this area of investigation.Abbreviations: Ax-antheraxanthin; NPQ-non photochemical quenching of chlorophyll fluorescence; PSII-photosystem II; VC-violaxanthin cycle; VDE-violaxanthin deepoxidase; Vxviolaxanthin; ZE-zeaxanthin epoxidase; Zx-zeaxanthin.

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“…Practical and theoretical tests presented by Kubínová et al (2014) demonstrated that the frequently used method for chloroplast number estimation by counting profiles of particles from 2D sections yielded biased estimates (e. g. Boffey et al, 1979;Miyazawa and Terashima, 2001;Sam et al, 2003;Zechmann et al, 2003;Wang et al, 2004;Hayashida et al, 2005;Oguchi et al, 2005;Teng et al, 2006;Image Anal Stereol 2017;35:187-205 Gopi et al, 2008;Maslova et al, 2009;Jin et al, 2011;Simon et al, 2013) and that the results may be one order of magnitude different from the real chloroplast numbers. Another possible method is to count the chloroplasts in cells directly during focusing through the specimen using conventional light microscopy (Ellis and Leech, 1985;Bockers et al, 1997) which can be applied if entire cells can be focused through and the chloroplasts are sparsely distributed in cells.…”

Section: Number Of Mesophyll Cells and Chloroplastsmentioning

confidence: 99%

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

Kubínová¹,

Radochová²,

Lhotáková³

et al. 2017

Image Anal Stereol

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This review presents an historical overview of stereological methods used for the quantitative evaluation of plant anatomical and cytological structures. It includes the origins of these methods up to the most recent developments such as the application of stereology based on 3D images. We focus especially on leaf, as the vast majority of studies of plant microscopic structure examine this organ. An overview of plant cell ultrastructure measurements as well as plant anatomical characteristics (e.g., plant tissue volume density, internal leaf surface area, number and mean size of mesophyll cells and chloroplast number), which were estimated by stereological methods most frequently, is presented. We emphasize the importance of proper sampling needed for unbiased measurements. Furthermore, we mention other methods used for plant morphometric studies and briefly discuss their relevance, precision, unbiasedness and efficiency in comparison with unbiased stereology. Finally, we discuss reasons for the sparse use of stereology in plant anatomy and consider the future of stereology in plant research.

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Seasonal structural and functional changes in the photosynthetic apparatus of evergreen conifers

Cited by 23 publications

References 20 publications

Patterns of spatio‐temporal distribution of winter chronic photoinhibition in leaves of three evergreen Mediterranean species with contrasting acclimation responses

Patterns of spatio‐temporal distribution of winter chronic photoinhibition in leaves of three evergreen Mediterranean species with contrasting acclimation responses

Current views on the function of the violaxanthin cycle (development of ideas put forward by D.I. Sapozhnikov)

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

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