Seasonal Variability in the Contents and Composition of Essential Oil in Various Thuja Species Occurring in Czechoslovakia

Buben, I.; M, Karmazin; Trojankova, J.; Nova, D.

doi:10.17660/actahortic.1992.306.21

Cited by 3 publications

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“…However, the content of thujones reported by Szołyga et al ( 2014) is much bigger (almost 80%). The same main components of T. occidentialis extract were found in studies published by Bozsik et al (2015), Tsiri et al (2009 and Buben et al (1992). In these studies transferruginol was not identified, unlike our study where trans-ferruginol was present.…”

Section: Resultssupporting

confidence: 75%

“…In these studies transferruginol was not identified, unlike our study where trans-ferruginol was present. Presented by Buben et al (1992), a chemotype of T. occidentalis collected in Kostelec in the Czech Republic in 1991 contained cis-thujone in the greatest amount, then sabinen, fenchone and trans-thujone. This chemotype is almost identical to the obtained extract.…”

Section: Resultsmentioning

confidence: 99%

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Acta Biologica Cracoviensia s. Botanica

Chajduk,

Gołębiowski

2021

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The market value of essential oils is steadily increasing every year. They are mainly used as aromas and preservatives in food and cosmetics industries. The content and yield of the obtained extracts change across the seasons of the year. The knowledge of these differences is important for essential oils industry to gain the best quality and quantity of products. In this study Thuja occidentalis L. (Eng. northern white cedar) extract was obtained from leaves by maceration in dichloromethane. Plant material was collected during each season of the year and the finished products were thoroughly examined through GC-MS analysis. The seasonal variations of volatile compound composition showed that the most adequate period to obtain T. occidentalis extract on an industrial scale is spring. This is due to the best efficiency (almost 10%) and the highest content of monoterpenes (67%).

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Acta Biologica Cracoviensia s. Botanica

Chajduk,

Gołębiowski

2021

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“…Shoots of European Yew collected once a month for one year from two locations exhibited wide variations in taxoid constituents due to genotype and location (Hook et al, 1999). Locality also affected the sabinene and thujone content of Thuja plicata J. Donn ex D. Don, Cupressaceae (Buben et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…Based on essential oils content, T. occidentalis L., T. standishii (Gord.) Carrière, and T. plicata were recommended for production by Buben et al (1992). Significant variation in leaf oil of J. virginiana was reported between sexes and between habitats within a single geographic region (Setzer et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Podophyllotoxin Content in Leaves of Eastern Red Cedar (Juniperus Virginiana)

Maqbool¹,

Cushman²,

Gerard³

et al. 2004

Acta Hortic.

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Eastern red cedar (Juniperus virginiana L. Cupressaceae) occurs naturally throughout eastern North America and has been identified as a source of podophyllotoxin. Podophyllotoxin is used to manufacture drugs for treatment of cancer, rheumatoid arthritis, genital warts, psoriasis, and multiple sclerosis. Leaf material was collected from Juniperus plants located in northern Mississippi once a month from April 2001 to March 2002. The objective of the survey was to examine variations in podophyllotoxin content due to sampling date and plant type. Samples of 100 g of fresh leaf tissue were collected from juvenile, immature, mature male, and mature female plants and then dried, ground, extracted in chloroform, and analyzed with HPLC to measure podophyllotoxin content. Leaf tissue was harvested from the same immature and mature plants each sampling date. Average podophyllotoxin content of juvenile plants was 0.60 mg•g-1 compared to approximately 1.45 mg•g-1 in immature and mature plant types. Reproductive status, male or female, did not affect podophyllotoxin content. Sampling date significantly affected podophyllotoxin content. Plants harvested in January and April exhibited the greatest podophyllotoxin content (1.56 and 1.45 mg•g-1 , respectively) and plants harvested in February and June exhibited the lowest content (1.06 and 1.08 mg•g-1 , respectively). Though differences due to sampling date were statistically significant, there appeared to be no obvious pattern or trend. There was no significant interaction between plant type and sampling date. These results indicate that juvenile leaf tissue should be avoided due to its lower podophyllotoxin content compared to that of mature leaf tissue. These results also indicate that podophyllotoxin content of Eastern red cedar can vary as much as 50 % over time, ranging from a low of 1.06 to a high of 1.56 mg•g-1 .

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Effects of Arborvitae (Thuja plicata) Essential Oil on Cervical Cancer Cells: Insights into Molecular Mechanisms

Piña-Cruz,

Molina-Pineda,

Aguila-Estrada

et al. 2024

ACAMC

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Aims: This study aimed to assess the effects of AEO in an in vitro model of cell lines derived from cervical cancer—namely, HeLa and SiHa—by screening for AEO’s cytotoxic properties and examining its influence on the modulation of gene expression. Background: Cervical cancer stands as a prevalent global health concern, affecting millions of women worldwide. The current treatment modalities encompass surgery, radiation, and chemotherapy, but significant limitations and adverse effects constrain their effectiveness. Therefore, exploring novel treatments that offer enhanced efficacy and reduced side effects is imperative. Arborvitae essential oil, extracted from Thuja Plicata, has garnered attention for its antimicrobial, anti-inflammatory, immunomodulatory, and tissue-remodeling properties; however, its potential in treating cervical cancer remains uncharted. Objective: The objective of this study was to delve into the molecular mechanisms induced by arborvitae essential oil in order to learn about its anticancer effects on cervical cancer cell lines. Methods: The methods used in this study were assessments of cell viability using WST-1 and annexin V– propidium iodide, mRNA sequencing, and subsequent bioinformatics analysis. Results: The findings unveiled a dose-dependent cytotoxic effect of arborvitae essential oil on both HeLa and SiHa cell lines. Minor effects were observed only at very low doses in the HaCaT non-tumorigenic human keratinocyte cells. RNA-Seq bioinformatics analysis revealed the regulatory impact of arborvitae essential oil on genes enriched in the following pathways: proteasome, adherens junctions, nucleocytoplasmic transport, cell cycle, proteoglycans in cancer, protein processing in the endoplasmic reticulum, ribosome, spliceosome, mitophagy, cellular senescence, and viral carcinogenesis, among others, in both cell lines. It is worth noting that the ribosome and spliceosome KEGG pathways are the most significantly enriched pathways in HeLa and SiHa cells. Conclusion: Arborvitae essential oil shows potential as a cytotoxic and antiproliferative agent against cervical cancer cells, exerting its cytotoxic properties by regulating many KEGG pathways.

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Seasonal Variability in the Contents and Composition of Essential Oil in Various Thuja Species Occurring in Czechoslovakia

Cited by 3 publications

References 0 publications

Acta Biologica Cracoviensia s. Botanica

Acta Biologica Cracoviensia s. Botanica

Podophyllotoxin Content in Leaves of Eastern Red Cedar (Juniperus Virginiana)

Effects of Arborvitae (Thuja plicata) Essential Oil on Cervical Cancer Cells: Insights into Molecular Mechanisms

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