Seasonal variability of detoxificant response and heavy metal accumulation in tissues of both sexes in Tinca tinca (L.) from Lake Trasimeno

Elia, Antonia Concetta; Dörr, Ambrosius Josef Martin; Abete, Maria Cesarina; Prearo, Marino

doi:10.1007/s11160-009-9143-0

Cited by 25 publications

(10 citation statements)

References 27 publications

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“…During this time, glutathione sequesters labile metal and prevents cytotoxicity (Singhal et al, 1987;Elia et al, 2008;Elia et al, 2010). In our study, the involvement of glutathione was not evident, but there are several reasons for this.…”

Section: Discussioncontrasting

confidence: 57%

Copper and zinc detoxification in Gammarus pulex (L.)

Khan

Bury

Högstrand

2012

Journal of Experimental Biology

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SUMMARY To negate the toxicity of labile intracellular metals, some aquatic organisms partition metals into specific subcellular locations for detoxification, namely the soluble heat-stable cytosol and insoluble metal-rich granules. The aim of the present study was to characterise these subcellular storage sites in the freshwater crustacean Gammarus pulex (Linnaeus) following in situ exposures upstream (Drym, low metal) and downstream (Relubbus, elevated metal) of copper- and zinc-rich inflows into the River Hayle (Cornwall, UK). In the cytosol of gammarids exposed at Relubbus, copper and zinc associated to a 7.5-kDa metallothionein-like protein (MTLP) that was largely absent from gammarids prior to exposure. Exposure at Relubbus caused MTLP concentrations to increase 4- to 5-fold between days 2 and 4, indicating an induction response to increased labile intracellular metal. On day 16, spherical calcium-rich granules (0.5–2.5 μmol l–1) were visualised and analysed in the posterior caeca of gammarids exposed at both sites. Following exposure at Relubbus, granules contained trace amounts of copper, but zinc was absent. Granules in gammarids exposed at Drym contained no detectable copper or zinc. Granule formation appeared to be independent of exposure. Within the posterior caeca, granules have been associated with calcium storage during the crustacean molt, rather than in detoxification of trace metals. However, the granular copper burden appeared to follow environmental Cu availabilities. Thus, we describe Cu sequestration within molt-cycle calcium storage granules. As both MTLP concentrations and granule formation in crustaceans are affected upon by molting, we hypothesise that detoxification might impact upon this existing process.

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Section: Discussioncontrasting

confidence: 57%

Copper and zinc detoxification in Gammarus pulex (L.)

Khan

Bury

Högstrand

2012

Journal of Experimental Biology

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“…Both kidney and liver tissues chosen for this study have high potential for ROS production and response of the oxidative stress biomarkers in fish is tissue-specific [60][61][62][63].…”

Section: Oxidative Stress Biomarkersmentioning

confidence: 99%

Could Dietary Black Soldier Fly Meal Inclusion Affect the Liver and Intestinal Histological Traits and the Oxidative Stress Biomarkers of Siberian Sturgeon (Acipenser baerii) Juveniles?

Caimi

Gasco

Biasato

et al. 2020

Animals

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The trial investigates if a highly defatted Hermetia illucens larva meal (H) at two dietary inclusion levels and a vegetable protein based diet (VEG) influences the normal gut and liver histology and the oxidative stress biomarkers in liver and kidney of Siberian sturgeon juveniles. Fish were fed four diets: one control diet (H0) containing 70% of fishmeal (FM), two diets including 18.5% (H185) and 37.5% (H375) of highly defatted H in substitution for 25% and 50% of FM, and one vegetable protein based diet (VEG). At the end of a growth trial, 12 fish per treatment were sacrificed by over-anaesthesia to collect 12 liver and 5 distal intestine samples for histological analyses, as well as 12 liver and kidney samples for biochemical analyses. The H and VEG diets did not significantly affect the histology of liver and distal intestine, but alterations of the oxidative stress biomarkers were detected at the highest inclusion level of H (37.5%). In order to avoid unfavorable effects on the fish health, an inclusion level up to 18.5% of H is recommended for Siberian sturgeon juveniles.

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“…Biomarker responses have been well characterized against a range of xenobiotics including disinfectants (Elia et al, 2006a(Elia et al, , 2008, trace metals (Elia et al, 2000(Elia et al, , 2003(Elia et al, , 2006b(Elia et al, , 2007(Elia et al, , 2010Al Kaddissi et al, 2014) and metal-containing NPs (Buffet et al, 2011, 2012. Exposure to Ag NPs resulted in an increase of oxidative stress and a corresponding depletion of antioxidant defense mechanisms (Carlson et al, 2008;Kim et al, 2009).…”

Section: Introductionmentioning

confidence: 99%