Seasonal variations of the chemical composition of Brazilian propolis

Bankova, Vassya; Boudourova-Krasteva, G.; Попов, Симеон; Sforcin, José Maurício; Funari, Sílvia Regina Cunha

doi:10.1051/apido:19980406

Cited by 128 publications

(101 citation statements)

References 9 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…18 Although the six samples were collected during different months, over the period of one year, no variation was observed in the chemical composition 19 nor in the antimicrobial activity 20,21 of samples of propolis from the Southeast of Brazil collected in different seasons. Therefore, although it was expected that the same basic composition would be found in the six samples analyzed, the results of each sample have been reported separately and the results compared only within each sample, for the sake of precision.…”

Section: Resultsmentioning

confidence: 98%

Factors that influence the yield and composition of Brazilian propolis extracts

Cunha¹,

Sawaya²,

Caetano³

et al. 2004

J. Braz. Chem. Soc.

154

View full text Add to dashboard Cite

Durante a extração de própolis foram variados alguns fatores para determinar como eles afetam o rendimento e o teor de fenóis totais. Seis amostras diferentes da região sudeste do Brasil foram testadas. Os resultados indicam que os maiores rendimentos de extratos de própolis obtidos por maceração, que também apresentam o maior número de componentes, foram aqueles usando 70% (v/v) ou maior proporção de etanol no solvente. A extração em Soxhlet resultou em um maior rendimento. Não foram observadas diferenças entre extratos de macerações com ou sem luz. Um aumento no rendimento da extração foi observado entre os extratos obtidos por maceração durante 10 e 30 dias, porém o teor de fenóis não variou significativamente nesses extratos. O teor de fenóis totais de todos os extratos variou entre 6,41 e 15,24% mas nenhuma correlação direta foi encontrada com qualquer um dos fatores testados.During the extraction of propolis several factors were varied in order to determine how they affected the yield and phenolic composition of the obtained extracts. Six samples of green propolis from the Southeastern region of Brazil were tested. The results indicate that the highest yield of propolis extracts obtained by maceration, which also had the greatest number of components, were those using 70% (v/v) ethanol or more as a solvent. The Soxhlet extraction procedure resulted in even higher yields. No differences were observed between extracts macerated in the presence or absence of light. An increase in yield was observed between extracts macerated for 10 and 30 days although the phenolic content did not vary significantly. The total phenolic content of all extracts varied from 6.41 to 15.24 % but no direct correlation could be found between any of the factors tested. Keywords: propolis, extraction techniques, high performance liquid chromatography IntroductionPropolis, a resinous substance collected by bees from plants around the hive, is masticated by the bees, salivary enzymes and beeswax added, then applied to the combs and walls of the hive, 1 thereby insulating and reinforcing the hives as well as making the environment aseptic. For beekeepers, propolis is a by-product obtained by scraping the walls of the hives. Crude propolis is composed basically of 55% vegetable resins and balsam, 30% bee wax, 10% essential oil and 5% pollen. 2 Due to its antibiotic and anti-fungal activity, propolis has been used in folk medicine for many centuries 3 and is presently used in health food and various pharmaceutical and cosmetic products such as mouthwash preparations, face creams, lotions and tablets. 1 Traditionally, the fraction soluble in 70% ethanol was extracted and referred to as propolis balsam. 3 In order to avoid the characteristic ethanolic smell and solubility problems of this product, propolis extracts using water 4 or other solvents such as glycerol, edible oils and propylene glycol 5 are presently found on the market. There is no standard either for extractive procedure or composition of products that contain propolis extracts...

show abstract

Section: Resultsmentioning

confidence: 98%

Factors that influence the yield and composition of Brazilian propolis extracts

Cunha¹,

Sawaya²,

Caetano³

et al. 2004

J. Braz. Chem. Soc.

154

View full text Add to dashboard Cite

show abstract

“…Telefax: (+5514) 3811-7189, E-mail: orsi@fca.unesp.br compounds (flavonoids, aromatic acids, benzopyranes), di-and triterpenes, essential oils, among others. Seasonal variations in propolis composition are not significant and are predominantly quantitative (2,4,6).…”

Section: Introductionmentioning

confidence: 97%

Synergistic effect of propolis and antibiotics on the Salmonella Typhi

Orsi¹,

Sforcin²,

Funari³

et al. 2006

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

The goal of this work was to investigate a possible synergistic effect between ethanolic extracts of propolis from Brazil and Bulgaria and some antibiotics (Amoxicillin, Ampicillin and Cefalexin) against Salmonella Typhi. Brazilian and Bulgarian propolis showed an antibacterial action, but the sample from Bulgaria was shown to be more efficient. Both samples showed a similar synergistic effect with these antibiotics. One may conclude that the propolis samples show an important antibacterial action, as well as a synergistic effect with antibiotics against Salmonella Typhi.

show abstract

“…Com relação à variação sazonal, a diminuição em alguns componentes biologicamente ativos, como no caso dos fenólicos, é acompanhada pelo aumento de outros, por exemplo, ácidos diterpênicos. Deste modo, pode-se esperar que algumas atividades biológicas, relacionadas a estes compostos (antibacteriana, antifúngica), sejam similares em diferentes estações do ano (Bankova et al, 1998). Park et al (2002) classificaram a própolis brasileira em doze tipos de acordo com a região geográfica de origem, composição química e vegetação de onde foi extraída.…”

Section: Introductionunclassified

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Nunes¹,

Galindo²,

Deus³

et al. 2009

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO:A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa acoplada com espectrometria de massas (CG-EM). Foram identificados 34 constituintes voláteis, sendo monoterpenos e monoterpenóides, sesquiterpenos e sesquiterpenóides, fenilpropanóides, aldeídos, cetonas e η-alcanos. Os constituintes majoritários foram o trans-anetol, α-copaeno e o metil cis-isoeugenol. Também foi realizado o perfil fitoquímico por cromatografia em camada delgada (CCD), através da qual os constituintes fenólicos foram identificados como majoritários. Com o extrato bruto metanólico da própolis, realizou-se o ensaio de letalidade em Artemia salina, que demonstrou DL 50 de 18,9 µg/mL, sugerindo uma possível atividade antitumoral. Unitermos: Própolis vermelha, constituintes voláteis, Artemia salina.ABSTRACT: "Seasonal variability of red propolis constituents and brine shrimp bioassay". Propolis is a resinous hive product collected by honeybees from plant sources. The composition of the propolis depends on the season, the vegetation, and the area of collection. It presents a diverse biological activities such as antimicrobial, antioxidant and antitumoral. The seasonal variability of the red propolis constituents from Pernambuco State were accomplished in the months of February, June and October of 2006. The volatile red propolis constituients were captured by the dynamic headspace technique and analyzed by GC-MS. In the analysis of the samples, 34 constituents were identified as the mono and sesquiterpens, phenylpropanoids, aldehydes, cetons and n-alcanes, presenting as majority constituents the trans-anethol, α-copaene and methyl cis-isoeugenol. The phytochemistry profile was performed by thin layer chromatography (TLC). Through this technique the majority constituents were identified as phenols. The Brine shrimp bioassay was used to evaluate of the red propolis methanolic extract, which demonstrated a DL 50 of 18.9 µg/mL and an activity antitumoral was possibly suggested.

show abstract

Seasonal variations of the chemical composition of Brazilian propolis

Cited by 128 publications

References 9 publications

Factors that influence the yield and composition of Brazilian propolis extracts

Factors that influence the yield and composition of Brazilian propolis extracts

Synergistic effect of propolis and antibiotics on the Salmonella Typhi

Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

Contact Info

Product

Resources

About