Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting

Esteban-Vives, Roger; Choi, Myung Sun; Young, Matthew P.; Over, Patrick; Ziembicki, Jenny; Corcos, A; Gerlach, Jörg C.

doi:10.1016/j.burns.2016.02.020

Cited by 29 publications

(26 citation statements)

References 26 publications

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“…Cells are isolated from a skin tissue (donor) and applied to the wound bed by spraying. This non‐invasive treatment strategy aims to facilitate the re‐epithelialization process, reducing the healing time in the hospital and minimizing complications . However, for the treatment of full‐thickness wounds a dermal element to achieve functional permanent skin is still required.…”

Section: Therapeutic Strategies For Skin Regenerationmentioning

confidence: 99%

Therapeutic strategies for skin regeneration based on biomedical substitutes

Chocarro‐Wrona

López‐Ruiz

Perán

et al. 2019

Acad Dermatol Venereol

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Regenerative medicine and tissue engineering (TE) have experienced significant advances in the development of in vitro engineered skin substitutes, either for replacement of lost tissue in skin injuries or for the generation of in vitro human skin models to research. However, currently available skin substitutes present different limitations such as expensive costs, abnormal skin microstructure and engraftment failure. Given these limitations, new technologies, based on advanced therapies and regenerative medicine, have been applied to develop skin substitutes with several pharmaceutical applications that include injectable cell suspensions, cell‐spray devices, sheets or 3Dscaffolds for skin tissue regeneration and others. Clinical practice for skin injuries has evolved to incorporate these innovative applications to facilitate wound healing, improve the barrier function of the skin, prevent infections, manage pain and even to ameliorate long‐term aesthetic results. In this article, we review current commercially available skin substitutes for clinical use, as well as the latest advances in biomedical and pharmaceutical applications used to design advanced therapies and medical products for wound healing and skin regeneration. We highlight the current progress in clinical trials for wound healing as well as the new technologies that are being developed and hold the potential to generate skin substitutes such as 3D bioprinting‐based strategies.

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Section: Therapeutic Strategies For Skin Regenerationmentioning

confidence: 99%

Therapeutic strategies for skin regeneration based on biomedical substitutes

Chocarro‐Wrona

López‐Ruiz

Perán

et al. 2019

Acad Dermatol Venereol

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“…The photo also reveals areas in the neck that represented third-degree burns, which were treated with split skin grafting, but where the cell graft was additionally sprayed into the gaps between the mesh. Information on the cell isolation and the clinical procedure is given in [7,8,14,15]…”

Section: Clinical Case Reportmentioning

confidence: 99%

“…Using a previously clinically applied cell isolation method, we have demonstrated that autologous freshly isolated HEK containing skin cell suspensions can be used for immediate spray-grafting with clinically satisfying results [7,8,14,15]. This method differs from the process published by of Wood et al in performing an initial step of separation of the dermal and epidermal layer using the enzyme dispase followed by an extra cell washing step [12].…”

Section: Introductionmentioning

confidence: 97%

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

Johnen¹,

Hartmann²,

Plettig³

et al. 2019

SCR

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A skin cell-spray grafting technique that enables the on-site application of freshly isolated autologous single cell suspensions was already applied in many cases on caucasian patients with low skin coloration. Our project hypothesis is that these suspensions contain keratinocytes and vital melanocytes, that are of particular interest for the treatment of patients of darker skin color. To test this, we applied an in vitro model, wherein the feasibility of i) isolating and ii) spraying of freshly isolated autologous melanocyte-keratinocyte cell suspensions was investigated. Primary human epidermal keratinocytes (HEKs) and melanocytes (MCs) were isolated from skin biopsies (n=8). Biochemical parameter, cell counts, cell morphology, growth behavior and immunofluorescence results were compared in two groups using MC cultures and co-cultures of MCs with HEKs. Case information on using the method clinically with one patient is included. The sprayed mixed cell suspensions proliferated in all groups without measurable loss of viability, and MCs exhibited a regular cell morphology in monoculture up to passage 4°. The sprayed MCs and HEKs demonstrated in vitro glucose and lactate metabolism that was comparable to the pipetted controls. In co-culture, well distributed CK14+ HEKs and NKI/beteb+ MCs could be demonstrated, which interacted in the in vitro model. The ratio of HEKs : MCs in our primary cultures were microscopically counted (n=8 each) as mean +/- SD 1,211,000 (+/- 574,343) HEK : 99,625 (+/- 59,025) MC; i.e., a ratio of approx. 12 : 1. Using the isolation method clinically for a patient with dark skin coloration after suffering severe second-degree burns shows a satisfying re-pigmentation of the resulting wound post healing. Freshly isolated spray-on melanocyte/keratinocyte suspensions provide for a considerable amount of viable HEKs and MCs. Using MCs in spray-grafting suspensions could represent a promising approach for treating severe partial-thickness burns and innovative therapy developments that also aim to address cosmetic aspects.

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“…However, technical problems exist for all of the previous spray devices, including facets of the spraying process and the effects of the spraying on the cells and their efficiency in wound repair (Veazey et al, 2005;Sosnowski et al, 2013). In addition, the previous spray devices were designed and manufactured in large sizes that minimize or obviate their portability and greatly limit application scenarios (Esteban-Vives et al, 2016a). Furthermore, the previous spray devices were found to generate problems caused by mechanical effets on the liquid that result in the formation of large droplets that limit the ability to generate a uniform cell delivery (Bahoric et al, 1997;Beneke et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing

Chang

Liu

Guo³

et al. 2020

Front. Bioeng. Biotechnol.

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Severe skin wounds are often associated with large areas of damaged tissue, resulting in substantial loss of fluids containing electrolytes and proteins. The net result is a vulnerability clinically to skin infections. Therapies aiming to close these large openings are effective in reducing the complications of severe skin wounds. Recently, cell transplantation therapy showed the potential for rapid re-epithelialization of severe skin wounds. Here, we show the improved effects of cell transplantation therapy using a robust protocol of efficient expansion and delivery of epidermal cells for treatment of severe skin wounds. Human skin tissues were used to generate human epidermal organoids maintained under newly established culture conditions. The human epidermal organoids showed an improved capacity of passaging for at least 10 rounds, enabling organoids to expand to cell numbers required for clinical applications. A newly designed auto micro-atomization device (AMAD) was developed for delivery of human epidermal organoids onto the sites of severe skin wounds enhancing uniform and concentrated delivery of organoids, facilitating their engraftment and differentiation for skin reconstitution. With the optimal design and using pneumatic AMAD, both survival and functions of organoids were effectively protected during the spraying process. Cells in the sprayed human epidermal organoids participated in the regeneration of the epidermis at wound sites in a mouse model and accelerated wound healing significantly. The novel AMAD and out new protocol with enhanced effects with respect to both organoid expansion and efficient transplantation will be used for clincal treatments of complex, uneven, or large-area severe skin wounds.

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Second-degree burns with six etiologies treated with autologous noncultured cell-spray grafting

Cited by 29 publications

References 26 publications

Therapeutic strategies for skin regeneration based on biomedical substitutes

Therapeutic strategies for skin regeneration based on biomedical substitutes

An in vitro feasibility investigation considering primary human melanocytes for spray- grafting of freshly isolated autologous skin cells for burn treatment and a clinical case report

Auto Micro Atomization Delivery of Human Epidermal Organoids Improves Therapeutic Effects for Skin Wound Healing

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