Second‐derivative synchronous spectrofluorimetric assay of dapagliflozin: Application to stability study and pharmaceutical preparation

Abstract

A highly accurate, simple and sensitive spectrofluorimetric analytical method for dapagliflozin (DGF) quantitation was developed. The proposed method was successively applied to DGF analysis in both its pure and pharmaceutical dosage forms. This method was developed to investigate DGF stability in its degradation products, as laid out in International Council for Harmonisation (ICH) rules. Kinetics of alkaline degradation of DGF was also calculated. The half‐life time (t1/2) of the reaction was 75.32 min. An alkaline degradation pathway was described. The present study involved measurement of the second‐derivative synchronous fluorescence intensity of DGF at Δλ = 30 nm. Peak amplitude was measured at 322 nm. Linear range of the calibration curve was 0.1–1.0 μg ml−1. Lower detection and quantitation limits were 0.023 and 0.071 μg ml−1, respectively, and indicated good sensitivity of the proposed method. Mean per cent recovery was 99.78 ± 1.78%. The proposed analytical approach was successfully applied to DGF in the quality control laboratory and would be suitable as a stability‐indicating assay.

A Turn-On–Type Fluorescence Resonance Energy Transfer Eco-friendly Method for Nitazoxanide Quantification in Pharmaceutical Dosage Form and Spiked Plasma: Evaluation of Greenness Profile Using Different Assessment Tools

“…Also, the reported electrochemical methods always need electrode pre-modification. Thus, we found it urgent to establish a simple and time-saving approach for NTZ determination [19][20][21][22][23][24]. Fluorimetry is a sensitive and relatively selective approach that has been applied for NTZ analysis.…”

Machine Learning-Assisted Synchronous Fluorescence Sensing Approach for Rapid and Simultaneous Quantification of Thiabendazole and Fuberidazole in Red Wine

“…Synchronous fluorescence spectrometry, a technique initially described by Lloyd [16] in 1971 to map fluorescence spectra in the presence of simultaneous scanning of both excitation and emission monochromators, has been widely utilized for the investigation of mixtures. The simultaneous fluorescence approach is used by a growing number of scientists for the determination of compounds in complicated matrices, and it may be combined with other techniques, such as derivative technique, to accomplish further spectral separations [17][18][19][20][21][22]. Among these, the constant-energy synchronous fluorescence approach utilized in this work can effectively simplify the spectra, narrow the spectral band, and eliminate the influence of scattered light by selecting an appropriate constant-energy difference, making it advantageous for the detection of mixtures.…”