Second-generation autologous chondrocyte transplantation: MRI findings and clinical correlations at a minimum 5-year follow-up

Kon, Elizaveta; Martino, Alessandro Di; Filardo, Giuseppe; Tetta, Cecilia; Busacca, Maurizio; Iacono, Francesco; Delcogliano, Marco; Albisinni, Ugo; Marcacci, Maurilio

doi:10.1016/j.ejrad.2010.04.002

Cited by 66 publications

(71 citation statements)

References 18 publications

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“…While a graft with negligible mechanical properties may be sufficient for filling focal defects, in which the surrounding healthy native cartilage may be sufficient to support joint loading, a graft for resurfacing the lateral or medial condyle would likely need to be at a more advanced stage of maturation to provide a higher degree of functionality. Interestingly, recent midterm follow-up studies have shown poorer clinical outcomes, in terms of defect filling and repair, of larger vs smaller defects treated with Hyalograft-C [14,3]. Based on a short culture period of only two weeks, the 50mm constructs engineered in our scaled-up bioreactor had 20% of the suture retention and 5-10% of the aggregate moduli of native cartilage tissue.…”

Section: Discussionmentioning

confidence: 93%

“…Hyalograft-C, a 2cm x 2cm x 0.15cm thick Hyaff-11 based cartilage graft, has been shown to provide a clinical improvement to young patients with focal cartilage defects [2,14,3]. While the current manufacturing process, based on conventional manual static cell/tissue culture techniques [15], may be sufficient to produce Hyalograft-C in its current size, we speculated that a perfusion bioreactor-based approach could improve the quality of larger sized grafts.…”

Section: Discussionmentioning

confidence: 99%

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Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing

Santoro¹,

Olivares²,

Brans³

et al. 2010

Biomaterials

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Apart from partial or total joint replacement, no surgical procedure is currently available to treat large and deep cartilage defects associated with advanced diseases such as osteoarthritis. In this work, we developed a perfusion bioreactor system to engineer human cartilage grafts in a size with clinical relevance for unicompartmental resurfacing of human knee joints (50mm diameter x 3mm thick). Computational fluid dynamics models were developed to optimize the flow profile when designing the perfusion chamber. Using the developed system, human chondrocytes could be seeded throughout large 50mm diameter scaffolds with a uniform distribution. Following two weeks culture, tissues grown in the bioreactor were viable and homogeneously cartilaginous, with biomechanical properties approaching those of native cartilage. In contrast, tissues generated by conventional manual production procedures were highly inhomogeneous and contained large necrotic regions. The unprecedented engineering of human cartilage tissues in this large scale opens the practical perspective of grafting functional biological substitutes for the clinical treatment for extensive cartilage defects, possibly in combination with surgical or pharmacological therapies to support durability of the implant. Ongoing efforts are aimed at integrating the up-scaled bioreactor-based processes within a fully automated and closed manufacturing system for safe, standardized, and GMP compliant production of large-scale cartilage grafts.2

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing

Santoro¹,

Olivares²,

Brans³

et al. 2010

Biomaterials

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“…Conversely, an incomplete graft infill and the persistence of edema-like signals have been found to translate into inferior clinical outcome. 5,21 The use of more detailed variables 31 3 63 3…”

Section: Discussionmentioning

confidence: 99%

Is Magnetic Resonance Imaging Reliable in Predicting Clinical Outcome After Articular Cartilage Repair of the Knee?

et al. 2013

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“…stiffness) of joint tissues vary among people in vivo , and so definitions of population-level baseline values may simply exhibit excessive variability to preclude interpretation of deviations from baseline at the level of the individual patient. However, it may be possible in the near-term to routinely apply functional imaging in animal and clinical trials of therapeutic interventions [22, 58–61], or in cases of (e.g. ACL) injury in patients, where defined steps of disease progression can be known, accelerated, and possibly prevented.…”

Section: Functional Imaging Of Biomechanics In Oamentioning

confidence: 99%

Functional imaging in OA: role of imaging in the evaluation of tissue biomechanics

Neu

2014

Osteoarthritis and Cartilage

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Functional imaging refers broadly to the visualization of organ or tissue physiology using medical image modalities. In load-bearing tissues of the body, including articular cartilage lining the bony ends of joints, changes in strain, stress, and material properties occur in osteoarthritis (OA), providing an opportunity to probe tissue function through the progression of the disease. Here, biomechanical measures in cartilage and related joint tissues are discussed as key imaging biomarkers in the evaluation of OA. Emphasis will be placed on the a) potential of radiography, ultrasound, and magnetic resonance imaging to assess early tissue pathomechanics in OA, b) relative utility of kinematic, structural, morphological, and biomechanical measures as functional imaging biomarkers, and c) improved diagnostic specificity through the combination of multiple imaging biomarkers with unique contrasts, including elastography and quantitative assessments of tissue biochemistry. In comparison to other modalities, magnetic resonance imaging provides an extensive range of functional measures at the tissue level, with conventional and emerging techniques available to potentially to assess the spectrum of preclinical to advance OA.

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Second-generation autologous chondrocyte transplantation: MRI findings and clinical correlations at a minimum 5-year follow-up

Cited by 66 publications

References 18 publications

Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing

Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing

Is Magnetic Resonance Imaging Reliable in Predicting Clinical Outcome After Articular Cartilage Repair of the Knee?

Functional imaging in OA: role of imaging in the evaluation of tissue biomechanics

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