Second harmonic generation microscopy: a powerful tool for bio-imaging

Aghigh, Arash; Bancelin, Stéphane; Rivard, Maxime; Pinsard, Maxime; Ibrahim, Heide; Légaré, François

doi:10.1007/s12551-022-01041-6

Cited by 54 publications

(23 citation statements)

References 142 publications

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“…This material exhibited arbitrary polarization, sometimes dextrorotatory, sometimes levorotatory, which is typical of spontaneous resolution . Although crystal diffraction should solve at last the solid-state structure, other innovative nonlinear spectroscopy methods, such as second harmonic generation (SHG), have recently been applied to spot conglomerates and imaging of anisotropic substances. , …”

Section: Resultsmentioning

confidence: 99%

Revisiting HomochiralversusHeterochiral Interactions through a Long Detective Story of a Useful Azobis-Nitrile and Puzzling Racemate

García de la Concepción,

Flores-Jiménez,

Cuccia

et al. 2023

Crystal Growth & Design

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This paper documents and reinvestigates the solid-state and crystal structures of 4,4′-azobis-4-cyanopentanoic acid (ACPA), a water-soluble azobis-nitrile of immense utility as a radical initiator in living polymerizations and a labile mechanophore that can be embedded within long polymer chains to undergo selective scission under mechanical activation. Surprisingly, for such applications, both the commercially available reagent and their derivatives are used as “single initiators” when this azonitrile is actually a mixture of stereoisomers. Although the racemate and meso compounds were identified more than half a century ago and their enantiomers were separated by classical resolution, there have been confusing narratives dealing with their characterization, the existence of a conglomeratic phase, and fractional crystallization. Our results report on the X-ray crystal structures of all stereoisomers for the first time, along with further details on enantiodiscrimination and the always intriguing arguments accounting for the stability of homochiral versus heterochiral crystal aggregates. To this end, metadynamic (MTD) simulations on stereoisomer molecular aggregates were performed to capture the incipient nucleation events at the picosecond time scale. This analysis sheds light on the driving homochiral aggregation of ACPA enantiomers.

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Section: Resultsmentioning

confidence: 99%

Revisiting HomochiralversusHeterochiral Interactions through a Long Detective Story of a Useful Azobis-Nitrile and Puzzling Racemate

García de la Concepción,

Flores-Jiménez,

Cuccia

et al. 2023

Crystal Growth & Design

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“…The third review article is a collaborative effort from researchers based in Canada and France which deals with a non-linear optical phenomenon known as second-harmonic microscopy (SHM) (Aghigh et al 2023). The underlying principle of SHM is second harmonic generation (SHG) which involves the interaction of two photons of the same frequency with the dipole of a non-centrosymmetric material.…”

Section: Precis Of the Current Issuementioning

confidence: 99%

“…SHM has been a popular topic within the journal, with a number of reviews on related topics being published over the last 15 years (Cox 2011;Kato 2019;Mizuguchi and Nuriya 2020;Pallen et al 2021). The current work describes the history of the SHM field and its use in examining various fibrous structures such as collagen and microtubules (Aghigh et al 2023).…”

Section: Precis Of the Current Issuementioning

confidence: 99%

Biophysical Reviews: Turning the page from 2022 to 2023

Hall

2023

Biophys Rev

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This Editorial (vol. 15 issue 1-Regular Issue featuring an Issue Focus on the "100 th Anniversary of Har Gobind Khorana") first describes the issue contents before providing both, a look back at some journal highlights from 2022, and a look forward to what we can expect from 2023. The Editorial closes with a roundup of new journal access features and an acknowledgement of those supporting the journal.As we take our first steps into the new year, we use this moment of transition to educate the readers of Biophysical Reviews about the journal, what we are trying to achieve, and how we are doing it.

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“…In addition to microscopic methods, optical coherence tomography (OCT) methods are used to characterize pathological tissue changes in tendons and cartilage tissue, with limited resolution to study individual fibril and cell structure [1,2]. Two-photon or multiphoton microscopy (MPM) and second harmonic generation (SHG) provide higher lateral resolution, greater penetration depth, and information on fibril orientation [3,4]. Real-time examination would be advantageous in in-vivo diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…To detect pathological tissue changes by MPM, two different physical phenomena are used. One is frequency doubling (SHG) and the other is two photon excited fluorescence (TPEF) [3,4,5]. These nonlinear optical effects occur selectively in certain biological tissue structures.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of a two-photon fluorescence microscope for collagen tissue based on a nanosecond laser

Baselt,

Scheller,

Fleck

et al. 2023

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI

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The structural and microscopic examination of cartilage and tendon tissue is of central interest for the characterization of torn ligament healing or pathological tissue changes. Conventional microscopy or optical coherence tomography methods can only partially resolve tissue types and their structural orientation. Two-or multiphoton microscopy and second harmonic generation provide information about the material composition or alignment of nanoscaled structural proteins. In most systems, femtosecond lasers are used, which leads to high system costs and requires special system components. The aim of this work is to investigate a narrowband nanosecond laser with a pulse energy several orders of magnitude higher, reducing the required number of laser pulses to be averaged per pixel. Second harmonic generation and two photon excited fluorescence can be used to non-invasively examine deeper tissue structures. High lateral resolution was achieved by scanning the sample. Simultaneous real-time visualization of collagenous and cellular structures was attained. Defined aligned collagenous fibers of a sheep tendon were investigated. The anisotropy of the collagenous structures could be demonstrated. It was possible to realize a two-dimensional imaging method with a maximum point density of 5080 PPI and a numerical aperture of 0.15. The method allows the simultaneous separate observation of collagen fibers through the use of second harmonic generation and cellular tissue using a narrow-band nanosecond laser for two photon excited fluorescence.

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Second harmonic generation microscopy: a powerful tool for bio-imaging

Cited by 54 publications

References 142 publications

Revisiting HomochiralversusHeterochiral Interactions through a Long Detective Story of a Useful Azobis-Nitrile and Puzzling Racemate

Revisiting HomochiralversusHeterochiral Interactions through a Long Detective Story of a Useful Azobis-Nitrile and Puzzling Racemate

Biophysical Reviews: Turning the page from 2022 to 2023

Development and characterization of a two-photon fluorescence microscope for collagen tissue based on a nanosecond laser

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