2000
DOI: 10.1016/s1044-0305(99)00145-2
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Secondary ion images of the rodent brain

Abstract: Sections of biologic tissue obtained from laboratory rodents are prepared and analyzed by secondary ion mass spectrometry. The intensity of phosphocholine secondary ions is used to identify anatomical features of the brain from secondary ion images and to evaluate the effectiveness of procedures developed. Secondary ion emission of phosphocholine (m/z 184), is found to be abundant and its intensity is heterogeneous. Effects of sample thickness are addressed. Correspondence between conventional optical images o… Show more

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Cited by 27 publications
(22 citation statements)
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“…Moreover, secondary m/z 184 images of adult rat brain show a direct correlation with optical images of stained tissue sections [8]. Regions of the brain containing a high density of cells such as cerebral cortex or the caudate/putamen yield an intense m/z 184 secondary ion signal, whereas acellular regions such as the corpus callosum or external capsule yield little or no m/z 184 secondary emission.…”
mentioning
confidence: 87%
See 1 more Smart Citation
“…Moreover, secondary m/z 184 images of adult rat brain show a direct correlation with optical images of stained tissue sections [8]. Regions of the brain containing a high density of cells such as cerebral cortex or the caudate/putamen yield an intense m/z 184 secondary ion signal, whereas acellular regions such as the corpus callosum or external capsule yield little or no m/z 184 secondary emission.…”
mentioning
confidence: 87%
“…ϫ 7.8 cm). Prior to tissue mounting copper slides undergo conventional subbing with gelatin similar to that normally done with glass slides [8]. Gelatin coating of copper slides used for SIMS imaging allows the freshly mounted section to remain flat while in the process of air drying.…”
Section: Section Preparationmentioning
confidence: 99%
“…However, SIMS is based on the outcome of the impact of a high energy primary ion onto a target, and the yield of large molecular fragments, necessary for the identification of the original molecule, seems to be limited [3]. Results presented in recent years are still at the level of localizing molecular fragments like phosphocholine (m=z ¼ 184) [4], vitamin A fragment-1 [5], or OH and CN [6].…”
Section: Introductionmentioning
confidence: 99%
“…Although imaging of biological tissues has been provided by secondary ion mass spectrometry [1][2][3][4][5][6][7][8][9][10][11][12], quantitation of isotope ratios in secondary ion mass spectrometry of biological tissue has been problematic.…”
mentioning
confidence: 99%