Secondary Metabolite from Endophytic Fungi Aspergillus Sp. The Leave Of Kunyit Putih (Curcuma zedoaria (Berg) Roscoe)

Abstract

The compound from endophytic fungi of Aspergillus sp. from leaves of kunyit putih (Curcuma zedoaria (Berg.) Roscoe) has been isolated. Isolation begins with cultivation ofAspergillus sp. in 18 L PDB’s media (Potato Dextrose Broth) for 28 days. The liquid cultivation medium was extracted by partitioning method using ethylacetate and then evaporated. The extract was separated and purified by chromatography techniques. Elucidation stucture of the isolated compound was analysis by spectroscopic method NMR 1D and 2D. Antibacterial activity of isolated compound was tested using the disc diffusion method at concentrations 2500, 1000, 500, and 125 ppm. The isolated compounds obtained in the form of a yellow oil (24.30 mg). The 13C NMR spectrum indicated 24 signals of carbon and base on analysis spectrum DEPT 135 showed 5 signal methynes carbon, 1 signals methylene, 9 signals of methyl and 9 signals quarternary carbon. These signals from 1H and 13C-NMR suggested that this compound contained aromatic group and four carbonyl. The isolated compound show antibacterial activity at concentration 2500 ppm which inhibition zone for E. coli, S. dysenteriae, S. aureus, B. subtilis were 10.3 ; 8.3; 8.4; and 7.8 mm, respectively. Based on the analysis result of NMR 1D and 2D, the compound was methyl 6-(5'-(2"-acetoxy-2”-methylpropanoyl)-3"- methyl-2'-(3"'-methylbutanoyl)phenyl)-3-methylbutanoate and has weak antibacterial activity.

Review: Eksplorasi Pemanfaatan Jamur Endofit pada Tanaman Curcuma dan Zingiber sebagai Penghasil Senyawa Antibakteri

“…Senyawa antibakteri yang dihasilkan oleh jamur endofit juga dapat diproduksi dengan fermentasi cair atau submerged fermentation. Penelitian yang dilakukan oleh Muharni et al (2016); Pansanit & Pripdeevech (2018) dalam memproduksi senyawa antibakteri dari jamur endofit menggunakan medium cair berupa Potato Dextrose Broth (PDB). Isolat jamur endofit sebelum melalui proses fermentasi, dikulturkan kembali dengan menggunakan media agar seperti Potato Dextrose Agar dan diinkubasi pada suhu ruang selama 7 hari.…”

“…Penelitian serupa juga pernah dilakukan oleh Muharni et al (2016) yang menemukan Aspergillus sp. sebagai kapang endofit pada tanaman kunyit putih (C. zedoaria) tersebut.…”

“…sebagai kapang endofit pada tanaman kunyit putih (C. zedoaria) tersebut. Pengujian aktivitas antibakteri yang dilakukan oleh Muharni et al (2016) dilakukan dengan mengekstraksi senyawa metabolit sekunder dari Aspergillus sp, kemudian dipartisi dengan menggunakan pelarut etil asetat. Metabolit sekunder tersebut diteteskan di kertas cakram dan diuji terhadap bakteri Gram-positif seperti S. aureus dan B. subtilis serta Gram-negatif seperti E. coli dan S. dysenteriae.…”

Effectiveness of Utilizing Miana Leaves (Coleus Scuttelarioides (L) Benth) on the Growth of Escherichia Bacteria (E. Coli) as a Cause of Diarrhea at Drinking Water Refill Center

“…The results showed that the miana leaf extract with high concentrations resulted in a wider inhibition zone than those with low concentrations [19]. Another study on miana leaves found that the ethanol extract of Coleus scutellarioides Linn has antibacterial activity on E. coli and S. aureus bacteria [23]. The dissertation by Palette Tandi found that purple miana leaf extract has a pharmacotherapy effect for mycobacterium tuberculosis infection [22].…”

Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae

“…Observations on the diameter of the clear zone around the paper disc indicated as an inhibitory reaction of the rhizobacteria against Xoo bacteria, and each test was performed four times. Antibiosis activity of supernatant was performed using paper disc diffusion method (Muharni et al 2016;Rahma et al 2018). Four biocontrols standard and rice growth promoter rhizobacterial isolates were used as positive control.…”