Secondary Metabolite Production by the Basidiomycete, Lentinus strigellus, under Different Culture Conditions

Abstract

The basidiomycete Lentinus strigellus was cultivated in three different culture media and the secondary metabolites produced under different culture conditions were isolated and identified. When cultivated in a liquid medium with peptone, L. strigellus afforded the benzopyrans, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6-methoxychromane and (3R,4S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. The indole alkaloid echinuline and the anthraquinone fiscione, both unprecedented for the genus Lentinus, were isolated from the mycelium of the fungus. When cultured in Czapek medium enriched with potato broth, the fungus afforded the same benzopyrans except (3S,4S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Panepoxydone and isopanepoxydone were also isolated when the microorganism was grown in Czapek medium.

Mycotherapy of the good and the tasty medicinal mushrooms Lentinus, Pleurotus, and Tremella

“…Lentinus strigellus was cultivated in three different media and the secondary metabolites produced under different culture conditions were isolated and identified by Barros-Filho et al, [34], when cultivated in a liquid medium with peptone, Lentinus strigellus produced benzopyrans, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6methoxychromane and (3R,4S)-3,4-dihydroxy-2,2-dimethyl-6methoxychromane. When Lentinus strigellus cultured in Czapek medium enriched with potato broth, the fungus produced the same benzopyrans except (3S,4S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane.…”

Mushroom-Derived Indole Alkaloids

“…108 This mushroom has been found to produce the red pyrroloquinolines mycenarubins D−F (117−119) as well as hematopodin (120) and hematopodin B (121). 108,109 Comparative metabolic profiling also indicated the presence of the aforementioned mycenarubin A (111) and sanguinolentaquinone (116). 108 The so-called black-edged bonnet, Mycena pelianthina, is a purple mushroom widely found throughout Europe and North America.…”

“…Lentinus strigellus , a medicinal mushroom from South America, has been found to produce the triprenylated tryptophan-based diketopiperazine echinulin ( 124 ) under a variety of culture conditions . This bioactive secondary metabolite has modest activity against Mycobacterium tuberculosis H37Ra (MIC value of 169.92 μM) and is cytotoxic to HeLa cells at 100 μg mL –1 . , Macrolepiotin ( 125 ), isolated from the poisonous mushroom Macrolepiota neomastoidea , comprises the dipeptide Trp-Ile coupled to lepiotin B ( 126 ) .…”

Lentinoids A–D, New Natural Products Isolated from Lentinus strigellus

“…strigellus [ 5 ]. Compounds such as strigellone A ( 5 ) [ 3 ], isopanepoxydone ( 6 ) [ 6 ], panepoxydone ( 7 ) [ 6 ], lentinoid E ( 8 ) [ 7 ] and other analogues have been reported from L. strigellus [ 8 ]. The lentinoids reported here have structural similarities with compounds isolated from species of the genera Panus ( rudis and conchatus [ 6 ]) and Lentinus ( strigosus [ 9 ] and connatus [ 10 ]).…”