Secondary metabolites changes in germinated barley and its relationship to anti-wrinkle activity

Abstract

The purpose of this research was to identify metabolite change during barley (Hordeum vulgare) germination and reveal active principles for the anti-wrinkle activity. Barley was germinated with deionized water (DW) and mineral-rich water (MRW) for the comparison of the effect of mineral contents on the metabolites changes during germination. The effects of germinated barley extracts (GBEs) on collagen production and collagenase inhibition were evaluated in vitro using human dermal fibroblasts (HDFs). A pronounced anti-wrinkle activity was observed in the test group treated with the MRW-GBEs. In order to find out the active components related to the anti-wrinkle activity, an orthogonal projection to latent structure-discriminant analysis (OPLS-DA) was performed, using the data from secondary metabolites profiling conducted by UPLC–PDA–ESI–MS. The anti-wrinkle activity of MRW-GBEs was revealed to be associated with the increase of oligomeric compounds of procyanidin and prodelphinidin, indicating that it can be used as an active ingredient for anti-wrinkle agents.

Anti‐wrinkle properties of Angelica gigas Nakai root extracts using mineral‐rich water

“…Mineral‐rich water is reported by changes metabolites for mineral contents compared to distilled water 16 . Germinated barley extracts with mineral‐rich water in Jeju revealed to be associated with the increase of procyanidin and prodelphinidin, indicating the anti‐wrinkle activity 9 . It should be noted that the content of mineral water in Jeju affects the antioxidant activity and anti‐aging effects, means that its utilization value is higher than that of other water resources 16 .…”

“…16 Germinated barley extracts with mineral-rich water in Jeju F I G U R E 2 Effects of type I collagen on Angelica gigas Nakai root extracts in human dermal fibroblasts revealed to be associated with the increase of procyanidin and prodelphinidin, indicating the anti-wrinkle activity. 9 It should be noted that the content of mineral water in Jeju affects the antioxidant activity and anti-aging effects, means that its utilization value is higher than that of other water resources. 16 Previously, we confirmed the anti-aging effects of A. gigas Nakai cultured in mineral-rich water.…”

“…Mineral‐rich water is abundant in minerals and nutrients derived from volcanic rocks. 9 It is a resource that can only be obtained in Jeju island of South Korea and made by natural filtering of seawater in the volcanic rocks. It contains a large content of minerals such as sodium, manganese, zinc, calcium, potassium to exist in the form of a mixture of seawater and groundwater.…”

Comprehensive Overview: The Effect of Using Different Solvents for Barley Extraction with Its Anti‐Inflammatory and Antioxidant Activity

“…The results showed that the pre‐treatment with β‐glucan could alleviate the gastric mucosal damage induced by ethanol [54] . Anti‐wrinkle and antimicrobial in addition to behavioral study are also specific activities measured for the ethanolic extracts [49,50,55] . Lee et al .…”

“…[54] Anti-wrinkle and antimicrobial in addition to behavioral study are also specific activities water China preventing chronic inflammation in cardiovascular diseases, Anti-oxidant (ORAC-FRAP) [30] water China DPPH and NO radical scavenging activity assays [17] measured for the ethanolic extracts. [49,50,55] Lee et al…”