Secondary metabolites from acridocarpus orientalis inhibits 4T1 cells and promotes mesenchymal stem cells (MSCs) proliferation

Jamshidi-Adegani, Fatemeh; Vakilian, Saeid; Rehman, Najeeb Ur; Al-Broumi, Mohammed; Al-Kindi, Juhaina; Alam, Khurshid; Mozafarinahavandi, Parisa; Hasan, Anwarul; Al-Riyami, Hamad; Hussain, Javid; White, S. C.; Al‐Harrasi, Ahmed; Al-Hashmi, Sulaiman

doi:10.1007/s11033-020-05632-y

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…No.4500-0220) for 30 min at room temperature. Cell cycle analysis was achieved utilizing a flow cytometer (guava easyCyte™ HT System, Millipore, Bedford, MA, USA) 51 .…”

Section: Methodsmentioning

confidence: 99%

Comparative study of the cytotoxicity, apoptotic, and epigenetic effects of Boswellic acid derivatives on breast cancer

Jamshidi-Adegani

Ghaemi

Al-Hashmi

et al. 2022

Sci Rep

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This study aimed to compare the effect of Boswellic acid derivatives on the viability, apoptosis, and epigenomic profiling of breast cancer. According to the viability assays, 3-O-acetyl-11-keto-β-Boswellic acid (AKBA) showed more toxicity against MDA-MB-231 cells when compared with the 3-O-acetyl-β-Boswellic acid (ABA). In contrast, ABA revealed less toxicity against MCF-10A. Cell cycle and apoptosis assays determined the maximum apoptotic effect of AKBA on MCF-7, and MDA-MB-231 cells. Interestingly, β-Boswellic acid (BA) and ABA did not promote the apoptosis in MCF-10A cells. Transwell migration assay indicated the greatest normalized inhibition (around 160%) in the migration of MDA-MB-231 cells induced by AKBA. The expression of P53, BAX, and BCL2 genes in cancerous cell lines has affirmed that both AKBA and ABA could induce the maximal apoptosis. Western-blot investigation demonstrated that the maximum over-expression of P53 protein (1.96 times) was caused by AKBA in MDA-MB-231 cells, followed by ABA in MCF-7 cells. The BCL2 protein expression was in agreement with the previously reported results. The global DNA methylation in both cancerous cells was reduced by ABA. These results suggest that ABA represented more epigenetic modulatory effect while AKBA shows more cytotoxic and apoptotic effect against breast cancer cell lines.

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“…No.4500-0220) for 30 min at room temperature. Cell cycle analysis was achieved utilizing a flow cytometer (guava easyCyte™ HT System, Millipore, Bedford, MA, USA) 51 .…”

Section: Methodsmentioning

confidence: 99%

Comparative study of the cytotoxicity, apoptotic, and epigenetic effects of Boswellic acid derivatives on breast cancer

Jamshidi-Adegani

Ghaemi

Al-Hashmi

et al. 2022

Sci Rep

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“…The survival of HUVECs seeded on the Geltrex‐coated membrane was further assessed using a LIVE/DEAD assay (LIVE/DEAD Viability/Cytotoxicity Kit, Thermo Fisher Scientific). [ 28 ]…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the viability of the HUVECs, astrocytes, and MDA-MB-231 cells was determined using a standard methyl thiazole tetrazolium bromide (MTT) assay kit (Bio-idea, Tehran, Iran). [28] 2.4 Cell cycle assay…”

Section: Cytotoxicity Evaluation Of β-Ba and Cismentioning

confidence: 99%

An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid

Vakilian

Alam

Al-Kindi

et al. 2021

Biotechnology Journal

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Background:The development of anti-cancer drugs with the ability to inhibit brain metastasis through the blood-brain barrier (BBB) is substantially limited due to the lack of reliable in vitro models.Main Methods: In this study, the Geltrex-based Transwell and microfluidic BBB models were applied to screen the effect of β-boswellic acid (β-BA) on the metastasis of MDA-MB-231 cells through the BBB in static and dynamic conditions, respectively.Major Results: The toxicity assay revealed that β-BA deteriorates MDA-MB-231 cells, while β-BA had no detectable toxic effects on human umbilical vein endothelial cells (HUVECs) and astrocytes. Trans-endothelial electrical resistance evaluation showed sustainable barrier integrity upon treatment with β-BA. Vimentin expression in HUVECs, evaluated using western blot, confirmed superior barrier integrity in the presence of β-BA. The obtained results were confirmed using an invasion study with a cell tracker and a scanning electron microscope. β-BA significantly inhibited metastasis by 85%, while cisplatin (Cis), a positive control, inhibited cancer cell migration by 12% under static conditions. Upon applying a dynamic BBB model, it was revealed that β-BA-mediated metastasis inhibition was significantly higher than that mediated by Cis. Conclusions and Implications:In summary, the current study proved the antimetastatic potential of β-BA in both static and dynamic BBB models.

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“…Further, the anti-proliferative activity of flavanoids was investigated on liver and colorectal cancer cells, 26,27 followed by the cytotoxic potential of morin flavonoids on MCF-7 and MDA-MB-231 breast cancer cell lines, 28,29 indicating that morin possess both anti-cancer and stem cell inducing activities. 30 Recently a study reported the role of morin and its zinc complexes on osteoblast differentiation with molecular level investigations on human osteoblast-like cells (MG63) and mouse mesenchymal stem cells (MSCs) (C3H10T1/2) with enhanced expression levels of Runx2, Type 1 collagen, osteocalcin (OCN) and osteonectin (ON), thus showing the potential osteogenic ability of morin. 31 Nonetheless, the specific molecular mechanisms have not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

The effects of morin on bone regeneration to accelerate healing in bone defects in mice

Wan

Jin

et al. 2020

Int J Immunopathol Pharmacol

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Restoring bone defects are the major challenge facing clinical trial therapy, particularly skull related problems. Morin, a naturally occurring compound, has pro-osteogenesis. This research focuses on assessing the role of morin for its pro-osteogenesis activities. We utilized in vivo and in vitro models to investigate the molecular-level mechanisms of morin’s osteoblastic biological activity. The effectiveness of morin on pro-osteogenesis (100 mg/kg/day) was assessed by monitoring modifications in the bone histomorphometry score, the development of immature osteoblasts from mesenchymal stems cells and improvements in the expression of pro-osteogenic cytokines in skull defected (SD) mice. Quantitative—PCR, Western blot analysis, and immunofluorescence were studied to investigate the signaling pathways. Morin has a substantial in vivo pro-osteogenesis effect which can facilitate the development of osteoblasts, the production of osteoblast related marker genes, and in vitro protein markers for osteoblasts. From a molecular biology standpoint, morin contributes to the development of osteoblasts and stimulation of the Wnt pathway with the activation and translocation of β-catenin nuclei. Our findings from the study revealed that morin may be a beneficial substitute for helping regenerate bone defects.

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Secondary metabolites from acridocarpus orientalis inhibits 4T1 cells and promotes mesenchymal stem cells (MSCs) proliferation

Cited by 4 publications

References 38 publications

Comparative study of the cytotoxicity, apoptotic, and epigenetic effects of Boswellic acid derivatives on breast cancer

Comparative study of the cytotoxicity, apoptotic, and epigenetic effects of Boswellic acid derivatives on breast cancer

An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid

The effects of morin on bone regeneration to accelerate healing in bone defects in mice

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