Secondary metabolites from Eurotium species, Aspergillus calidoustus and A. insuetus common in Canadian homes with a review of their chemistry and biological activities

Slack, G.; Puniani, Eva; Frisvad, Jens Christian; Samson, Robert A.; Miller, J. David

doi:10.1016/j.mycres.2008.12.002

Cited by 90 publications

(91 citation statements)

References 58 publications

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“…Echinulin was first discovered in A. brunneus (= E. echinulatum) by Quilico and Panizzi (1943). It was subsequently detected in many more section Aspergillus species (Ali et al 1989;Almeida et al 2010;Greco et al 2015;Li et al 2008b;Slack et al 2009;Smetanina et al 2007;Vesonder et al 1988) and shown to be toxic to animal cells (Ali et al 1989;Umeda et al 1974), while swine and mice respectively refused feed and water containing echinulin (Vesonder et al 1988). The presence of echinulin in the environment is not well documented however.…”

Section: Discussionmentioning

confidence: 99%

“…Species of section Aspergillus produce many extrolites exhibiting a wide range of biological activities (Frisvad and Larsen 2015a;Gomes et al 2012;Kanokmedhakul et al 2011;Li et al 2008a;Li et al 2008b;Slack et al 2009;Smetanina et al 2007). Most notably, compounds from A. chevalieri were shown to be active against Plasmodium falciparum (malaria), Mycobacterium tuberculosis and cancer cell lines (Kanokmedhakul et al 2011), an antitumor compound was reported from A. cristatus (Almeida et al 2010), while many compounds are known to be antioxidants.…”

Section: Introductionmentioning

confidence: 99%

“…Most notably, compounds from A. chevalieri were shown to be active against Plasmodium falciparum (malaria), Mycobacterium tuberculosis and cancer cell lines (Kanokmedhakul et al 2011), an antitumor compound was reported from A. cristatus (Almeida et al 2010), while many compounds are known to be antioxidants. They also produce mycotoxins, especially echinulin, flavoglaucin and physcion, which are toxic to animals (Ali et al 1989;Bachmann et al 1979;Cole and Cox 1981;Greco et al 2015;Nazar et al 1984;Rabie et al 1964;Semeniuk et al 1971;Slack et al 2009;Vesonder et al 1988), but toxicity has not been reported in humans. These species are not considered significant human pathogens, because most infections are superficial, with few cases of invasive infections known (de Hoog et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Species commonly grow as saprobes on clinical specimens, such as skin and nails (Hubka et al 2012). The biggest concern to humans, or nuisance, is the growth of these species inside homes, where exposure to spores and fragments, which contains β-(1, 3)-D-glucan, and other metabolites, cause allergies (Green et al 2006;Slack et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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A survey of xerophilic Aspergillus from indoor environment, including descriptions of two new section Aspergillus species producing eurotium-like sexual states

Visagie¹,

Yılmaz²,

Renaud³

et al. 2017

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Xerophilic fungi grow at low water activity or low equilibrium relative humidity and are an important part of the indoor fungal community, of which Aspergillus is one of the dominant genera. A survey of xerophilic fungi isolated from Canadian and Hawaiian house dust resulted in the isolation of 1039 strains; 296 strains belong to Aspergillus and represented 37 species. Reference sequences were generated for all species and deposited in GenBank. Aspergillus sect. Aspergillus (formerly called Eurotium) was one of the most predominant groups from house dust with nine species identified. Additional cultures deposited as Eurotium were received from the Canadian Collection of Fungal Cultures and were also re-identified during this study. Among all strains, two species were found to be new and are introduced here as A. mallochii and A. megasporus. Phylogenetic comparisons with other species of section Aspergillus were made using sequences of ITS, β-tubulin, calmodulin and RNA polymerase II second largest subunit. Morphological observations were made from cultures grown under standardized conditions. Aspergillus mallochii does not grow at 37 °C and produces roughened ascospores with incomplete equatorial furrows. Aspergillus megasporus produces large conidia (up to 12 µm diam) and roughened ascospores with equatorial furrows. Cobus M. Visagie et al. / MycoKeys 19: 1-30 (2017) 2

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A survey of xerophilic Aspergillus from indoor environment, including descriptions of two new section Aspergillus species producing eurotium-like sexual states

Visagie¹,

Yılmaz²,

Renaud³

et al. 2017

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“…The strains of C. cladosporioides confirmed their lack of secondary metabolite production, with only sporadically presence of emodin (Ogórek et al, 2012;Pitt and Hocking, 2009). All the Eurotium strains showed the production of the metabolite neoechinulin A (Butinar et al, 2005;Slack et al, 2009). As regards of E. halophilicum strains, some of them were characterized by the production of pseurotin A and D and tryprostatin B, already known as typical metabolites of A. fumigatus (Huang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Micheluz

Sulyok²,

Manente

et al. 2016

WMJ

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The secondary metabolite production of several fungal strains of Aspergillus creber, Aspergillus jensenii, Aspergillus penicillioides, Aspergillus protuberus, Aspergillus vitricola, Cladosporium cladosporioides, Eurotium chevalieri, Eurotium halophilicum, Penicillium brevicompactum and Penicillium chrysogenum were characterised by liquid chromatography tamdem mass spectometry. All fungi were isolated from both air and book covers as well as from settled dust from a contaminated library in Venice (Italy). For A. creber and A. jensenii, we identified sterigmatocystin, methoxysterigmatocystin, versicolorin A and related precursors/side metabolites from the biosynthetic pathways. Deoxybrevianamid E, neoechinulin A, pseurotin A and D, and rugulusovin were principally detected from the strains of E. halophilicum, an emerging fungal species implicated in book contaminations in specific indoor niches. The analysis of settled dust showed a wide range of toxic or bioactive fungal metabolites. Forty-five different metabolites were identified in different concentrations; in particular, high amounts of asperglaucide, alamethicin, andrastin A, terrecyclic acid and neoechinulin A were detected. Also one bacterial metabolite, chloramphenicole was detected. This study increases the knowledge about metabolite production of several fungal species, as well as on the indoor presence of fungi that are not detected by aerobiological sampling. These results emphasise how routine dusting operations are necessary and essential in order to prevent further microbiological developments in library environments.

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Production of New Cladosporin Analogues by Reconstitution of the Polyketide Synthases Responsible for the Biosynthesis of this Antimalarial Agent

et al. 2015

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The antimalarial agent cladosporin is an anomolar inhibitor of the Plasmodium falciparum lysyl-tRNAs ynthetase,a nd exhibits activity against both blood-and liver-stage infection. Cladosporin can be isolated from the fungus Cladosporium cladosporioides,w here it is biosynthesized by ah ighly reducing (HR) and an on-reducing (NR) iterative type Ipolyketide synthase (PKS) pair.Genome sequencing of the host organism and subsequent heterologous expression of these enzymes in Saccharomyces cerevisiae produced cladosporin, confirming the identity of the putative gene cluster. Incorporation of ap entaketide intermediate analogue indicated a5 + +3a ssembly by the HR PKS Cla2 and the NR PKS Cla3 during cladosporin biosynthesis.A dvanced-intermediate analogues were synthesized and incorporated by Cla3 to furnish new cladosporin analogues.Aputative lysyl-tRNA synthetase resistance gene was identified in the cladosporin gene cluster.A nalysis of the active site emphasizes key structural features thought to be important in resistance to cladosporin.Cladosporin (1;a sperentin) is at ricyclic octaketide that is produced by several fungal species,i ncluding Cladosporium, [1][2][3][4] Chaetomium, [5] Penicillium, [6] Eurotium, [7,8] and Aspergillus.[9] Cladosporin exhibits interesting bioactivity, including antifungal, antibiotic,a nd plant-growth inhibitory properties and anti-inflammatory responses in mouse lung tissue.[10] Recently,cladosporin has been shown to be ananomolar inhibitor of Plasmodium falciparum blood-and liverstage proliferation.[11] Although many antimalarial agents currently exist, endoperoxides are the only class of molecules for which resistance has not significantly developed, and even these do not inhibit the asymptotic liver-stage infection. The bioactivity of cladosporin represents apromising lead for the treatment of malaria, and several studies on this topic have been published. [12,13] Previously,w ei nvestigated the biosynthesis of several related fungal polyketides that belong to the resorcylic acid lactone (RAL) and dihydroxyphenylacetic acid lactone (DAL) containing polyketides,i ncluding hypothemycin (RAL type), [14] radicicol (RAL type), [15] and dehydrocurvularin (DAL type). [16][17][18][19] Biosynthesis of these polyketides requires cooperative action of two iterative type Ipolyketide synthases (PKSs): ah ighly reducing (HR) PKS and an onreducing (NR) PKS.B ased on structural similarities,w e hypothesized that cladosporin is also biosynthesized by aHR and an NR PKS.O ur early work assigned the absolute stereochemistry of cladosporin. [2,3] More recently,t he total synthesis of cladosporin and its diastereomer,isocladosporin, has been reported. [20,21] To better understand PKS assembly and enable analogue production by synthetic biology,w e sought to heterologously express and reconstitute cladosporin expression in Saccharomyces cerevisiae.Hence,wesequenced the genome of the producer, Cladosporium cladosporioides UAMH 5063. This resulted in 30 Mb of genomic information over at otal of...

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Secondary metabolites from Eurotium species, Aspergillus calidoustus and A. insuetus common in Canadian homes with a review of their chemistry and biological activities

Cited by 90 publications

References 58 publications

A survey of xerophilic Aspergillus from indoor environment, including descriptions of two new section Aspergillus species producing eurotium-like sexual states

A survey of xerophilic Aspergillus from indoor environment, including descriptions of two new section Aspergillus species producing eurotium-like sexual states

Fungal secondary metabolite analysis applied to Cultural Heritage: the case of a contaminated library in Venice

Production of New Cladosporin Analogues by Reconstitution of the Polyketide Synthases Responsible for the Biosynthesis of this Antimalarial Agent

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