Secondary metabolites of Curvularia sp. G6-32, an endophyte of Sapindus saponaria, with antioxidant and anticholinesterasic properties

Abstract

In the present study the extract of the secondary metabolites (E-G6-32) produced by the Curvularia sp. G6-32 endophyte (isolated from the medicinal plant Sapindus saponaria L.) and its biological activities were investigated. The antioxidant potential was confirmed by the DPPH (22.5%) and ABTS (62.7%) assays; the total phenolic compound content was 40 µg gallic acid equivalents/mg. The extract E-G6-32 displayed good inhibitory activity toward butyrylcholinesterase (BuChE; IC 50 = 110 ± 0.05 µg mL -1 ). The extract E-G6-32 was subjected to spectroscopic and mass spectrometry analyses ( 1 H and 13 C NMR, HMBC, HSQC, COSY, and MS/MS). Comparison with the literature data confirmed that (-)-asperpentyn (1) was a major component. Asperpentyn belongs to the epoxyquinone family that has attractive structural complexity, diverse functional groups, and a broad range of biological activities, including specific enzyme inhibitory activity. Our results suggest that Curvularia sp. G6-32 is a promising source of bioactive secondary metabolites that contain (-)-asperpentyn, which has potential pharmaceutical interest.

Bioactive compounds of Curvularia species as a source of various biological activities and biotechnological applications

“…G6-32 is an endophyte of the medicinal plant Sapindus saponaria . This compound has attractive structural complexity, functional groups diversity, and a wide range of biological activities like specific enzyme inhibitory activity (Polli et al, 2021 ). When Curvularia lunata was grown on malt extract broth in laboratory conditions, it produced three perylenequinones named methylated 12-epi-stemphytriol, alterperylenol, and dihydroalterperylenol.…”

Phylogenetic diversity and antioxidant activity of selected fungi from ethno-medicinal plants and soil

“…In addition, phomoterpene A, phomoisocoumarin D isolated from Diaporthe prunorum, an endophytic fungi isolated from Hypericum ascyron, has potential antimicrobial activity (Qu et al 2020), while Curvularia sp. G6-32, an endophytic isolate of medicinal plant Sapindus saponaria, produces Asperpentyn, an epoxyquinone compound, which is a potential antioxidant (Polli et al 2020).…”

Técnicas De Identificação Química De Metabólitos Produzidos Por Microrganismos Endofíticos

“…Por definição, os microrganismos endofíticos, são fungos e bactérias que passam pelo menos uma parte de seu ciclo de vida colonizando inter ou intracelularmente os tecidos de plantas, não causando sintomas de doença ao seu hospedeiro (ZHAO et al 2011;BERTANI et al 2016;CHAUHAN et al 2019). Estes microrganismos têm ganhado destaque por suas aplicações biotecnológicas (ZHAO et al 2011), especialmente na agricultura (OLIVEIRA et al 2020;EMMER et al 2021;OLIVEIRA et al 2021;RIBEIRO et al 2021), medicina e indústria com a produção de metabólitos bioativos (STROBEL, 2018;GOLIAS et al 2019;POLLI et al 2020;SILVA et al 2020;SILVA et al 2021) e/ou enzimas (DA SILVA RIBEIRO et al 2018;FELBER et al 2019;SANTOS et al 2019), além de atuarem como biorremediadores de locais contaminados com substâncias recalcitrantes e xenobióticas (BULLA et al 2017).…”

“…Após a obtenção do caldo fermentado, realiza-se a separação das células microbianas do caldo fermentado por centrifugação e/ou filtração e, do caldo fermentado resultante ocorre a extração dos metabólitos secundários empregando solventes orgânico adequados. O principal solvente empregado neste processo de extração é o Acetato de Etila (POLONIO et al 2016;SANTOS et al 2017;GOLIAS et al 2019;POLLI et al 2020;BALBINOT et al 2021;OLIVEIRA et al 2020;SILVA et al 2021). O solvente resultante da extração contendo os metabólitos secundários passa pelo processo de evaporação para a concentração dos metabólitos.…”